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Detailed Vector Information: pJFT7_nHalo_DC(r3)

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pJFT7_nHalo_DC(r3)              
Source: Center for Personalized Diagnostics
Description: In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments: None
Publications: None
Authors: Joshua LaBaer
Center for Personalized Diagnostics
Arizona State University
Ji Qiu
Fernanda Festa
Justin Saul
EvNO00621024

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DB3.1 T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Host Type:  bacterial    Marker:  chloramphenicol
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pJFT7_nHalo_DC(r3)

pJFT7_nHalo_DCr3 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  HaloseqF
Reverse:  pCPD6268_R
Description: In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments: One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp): 6229
Parent Vector: None
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Center for Personalized Diagnostics
Arizona State University
Ji Qiu
Fernanda Festa
Justin Saul
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 3514 4196
death cassette ccdB (BsrGI removed) ccdB death cassette - removed in plasmids containing a gene insert 2692 2997
enhancer CITE CITE enhancer 17 515
primer forward primer Forward sequencing primer HaloseqF aagcctgcctaactgcaa 1289 1360
primer reverse primer Reverse sequencing primer pCPD6268_R agccaactcagcttccttt 3206 3224
promoter T7 T7 promoter 6205 6229
protease cleavage site TEV TEV protease cleavage site 1422 1442
recombination site attR1 attR1 recombination site 1458 1482
recombination site attR2 attR2 recombination site 3138 3162
reporter gene LacZ LacZ alpha 6050 6118
selectable marker AmpR ampicillin resistance 4294 4953
selectable marker CmR chloramphenicol resistance - removed in plasmids containing a gene insert 1691 2347
ssDNA origin f1 F1 ssDNA origin 5722 6028
tag HALO N-terminal TEV-cleavable Halo tag 519 1409
trxn termination sequence T7 term T7 transcription termination 3285 3332

 

Species Specific ID: None
Target GenBank: None