Detailed Vector Information: pRSET-natGFP

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pRSET-natGFP
Source:
Center for Membrane Proteins of Infectious Diseases
Description:
Bacterial expression vector with a T7 promoter; adds C-terminal GFP tag; ampicillin resistance in bacteria; ligation independent cloning
Comments:
None
Publications:
PMID: 24593131
Title: Purification and Biophysical Characterization of the CapA Membrane Protein FTT0807 from Francisella tularensis.
PMID: 26908053
Title: Polyclonal Antibody Production for Membrane Proteins via Genetic Immunization
Authors:
Arizona State University
Center for Membrane Proteins in Infectious Diseases
PSI:Biology
EvNO00623703
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Special MTA: PSI

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.