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Detailed Vector Information: pJFT7_nHalo_DC(r4)

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pJFT7_nHalo_DC(r4)              
Source: Center for Personalized Diagnostics
Description: In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments: None
Publications: None
Authors: Joshua LaBaer
Center for Personalized Diagnostics
Arizona State University
Ji Qiu
Fernanda Festa
Justin Saul
Andrea Throop
EvNO00623723

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: HALO

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DB3.1 T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pJFT7_nHalo_DC(r4)

pJFT7_nHalo_DCr4 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  HaloseqF
Reverse:  pant1627r
Description: In vitro expression vector with a T7 promoter and N-terminal TEV-cleavable Halo tag; ampicillin resistance in bacteria; Gateway or Flexi cloning compatible
Comments: One in a series of pJFT7 vectors made by J. Saul for IVTT protein expression at CPD. This vector has been modified to improve sequencing the insert with a reverse primer.
Size (bp): 6140
Parent Vector: None
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Joshua LaBaer
Center for Personalized Diagnostics
Arizona State University
Ji Qiu
Fernanda Festa
Justin Saul
Andrea Throop
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3425 4107
death cassette ccdB ccdB death cassette (removed in plasmids containing a gene insert) 2785 2994
enhancer CITE CITE enhancer 17 515
protease cleavage site TEV TEV protease cleavage site 1422 1442
recombination site attR1 attR1 recombination site 1458 1474
recombination site attR2 recombination site 3145 3160
selectable marker AmpR ampicillin resistance 4205 4864
selectable marker CmR chloramphenicol resistance (removed in clones containing a gene insert) 1691 2347
ssDNA origin f1 f1 origin 5633 5939
tag HALO N-terminal HALO tag 522 1409

 

Species Specific ID: None
Target GenBank: None