BF3900 (B. fragilis )
In pET21_NESG (His-tagged bacterial expression vector)

Explanation of Terms

Gene:
Gene Symbol:  BF3900
Gene Name:  putative exopolyphosphatase-related protein
Original Clone ID:
BfR192-21.2
Keyword:
None
Species:
Bacteroides fragilis
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 24971458
Title: Comparing chemistry to outcome: the development of a chemical distance metric, coupled with clustering and hierarchal visualization applied to macromolecular crystallography.
Authors:
PSI
Northeast Structural Genomics Consortium
BfCD00627674
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AVAILABLE
No restriction
Special MTA: PSI/NESG
Insert sequence: 1029nts
Open reading frame: 1 to 1029

ATGCTGACTAAAGTGATTGCTCAGGCTCATATTGATCATTTTACCAAATGGTTTGAACGG
GCCGACAAAATAGTAATTGTATCTCATGTGTCACCTGATGGAGATGCCATTGGTTCATCA
TTGGGATTGTATCATTTTTTGGATTCTCAAGATAAAATTGTTAATGTGATTGTCCCCAAT
GCTTTTCCCGATTTTTTGAAATGGATGCCGGGTAGTAAGGATATTTTGCTGTATGACCGT
TACCAGGAATTTGCCGACAAGCTGATTATGGAGGCGGATGTCATTTGTTGTCTTGACTTT
AATGCGCTGAAGCGTATTGATGAAATGAGTGATATAGTGGCCGCTTCACCGGGGCGCAAA
ATAATGATAGACCACCATCTTTATCCTGAAGATTTTTGCAGAATCACCATTTCACATCCT
GAGATTTCCTCTACTTCCGAACTGGTGTTTCGATTGATTTGCCGGATGGGATATTTTAGT
GATATCTCCAAAGAAGGAGCCGAATGTATCTATACGGGCATGATGACAGATACCGGAGGG
TTCACTTATAACTCTAATAATCGTGAAATCTATTTCATCATCAGTGAGTTACTCTCAAAA
GGAATCGATAAAGATGATATTTACCGGAAAGTCTACAATACTTATTCTGAAAGCCGTTTG
CGTCTGATGGGATATGTGTTATCGAATATGAAGGTGTATAAAGATTACAATTCCGCCCTT
ATTTCACTGACAAAGGAAGAACAGGGCAAATTTGATTATATCAAAGGGGATAGCGAGGGG
TTTGTCAACATACCTCTGTCTATCAAGAATGTCTGTTTTTCTTGTTTTCTACGTGAGGAC
ACTGAAAAGAAAATGATTAAGATTTCTCTCCGTTCAGTAGGTAAGTTTCCTTGTAATCGG
TTAGCTGCGGAATTTTTCAATGGCGGCGGGCATCTCAATGCGTCCGGAGGGGAATTTTAC
GGTACTATGGAAGAGGCTGTAAAAGTGTTTGAACAAGCATTGGAGAAATATAAACCACTT
TTGAAAGAA

Protein sequence predicted based on nucleotide sequence

MLTKVIAQAHIDHFTKWFERADKIVIVSHVSPDGDAIGSSLGLYHFLDSQDKIVNVIVPN
AFPDFLKWMPGSKDILLYDRYQEFADKLIMEADVICCLDFNALKRIDEMSDIVAASPGRK
IMIDHHLYPEDFCRITISHPEISSTSELVFRLICRMGYFSDISKEGAECIYTGMMTDTGG
FTYNSNNREIYFIISELLSKGIDKDDIYRKVYNTYSESRLRLMGYVLSNMKVYKDYNSAL
ISLTKEEQGKFDYIKGDSEGFVNIPLSIKNVCFSCFLREDTEKKMIKISLRSVGKFPCNR
LAAEFFNGGGHLNASGGEFYGTMEEAVKVFEQALEKYKPLLKE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
NGS Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in BfR192
Recommended expression in:
Not Applicable