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CTSZ (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CTSZ
Gene Name:  cathepsin Z
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH020128.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000629

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 912nts         Open reading frame : 1 to 912
Reference Sequence Alignment

ATGGCGAGGCGCGGGCCAGGGTGGCGGCCGCTTCTGCTGCTCGTGCTGCTGGCGGGCGCG
GCGCAGGGCGGCCTCTACTTCCGCCGGGGACAGACCTGCTACCGGCCTCTGCGGGGGGAC
GGGCTGGCTCCGCTGGGGCGCAGCACATACCCCCGGCCTCATGAGTACCTGTCCCCAGCG
GATCTGCCCAAGAGCTGGGACTGGCGCAATGTGGATGGTGTCAACTATGCCAGCATCACC
CGGAACCAGCACATCCCCCAATACTGCGGCTCCTGCTGGGCCCACGCCAGCACCAGCGCT
ATGGCGGATCGGATCAACATCAAGAGGAAGGGAGCGTGGCCCTCCACCCTCCTGTCCGTG
CAGAACGTCATCGACTGCGGTAACGCTGGCTCCTGTGAAGGGGGTAATGACCTGTCCGTG
TGGGACTACGCCCACCAGCACGGCATCCCTGACGAGACCTGCAACAACTACCAGGCCAAG
GACCAGGAGTGTGACAAGTTTAACCAATGTGGGACATGCAATGAATTCAAAGAGTGCCAC
GCCATCCGGAACTACACCCTCTGGAGGGTGGGAGACTACGGCTCCCTCTCTGGGAGGGAG
AAGATGATGGCAGAAATCTACGCAAATGGTCCCATCAGCTGTGGAATAATGGCAACAGAA
AGACTGGCTAACTACACCGGAGGCATCTATGCCGAATACCAGGACACCACATATATAAAC
CATGTCGTTTCCGTGGCTGGGTGGGGCATCAGTGATGGGACTGAGTACTGGATTGTCCGG
AATTCATGGGGTGAACCATGGGGCGAGAGAGGCTGGCTGAGGATCGTGACCAGCACCTAT
AAGGATGGGAAGGGCGCCAGATACAACCTTGCCATCGAGGAGCACTGTACATTTGGGGAC
CCCATCGTTTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1152
Start on reference sequence 241
Mutations:
None
Discrepancy:
c143g,g144c,T48S
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CTSZ
Symbol Nomenclature : CTSZ
SYNONYM : CTSX
Designation : carboxypeptidase LB|cathepsin B2|cathepsin IV|cathepsin P|cathepsin X|cathepsin Y|cathepsin Z1|cysteine-type carboxypeptidase|lysosomal carboxypeptidase B|preprocathepsin P
Full Nomenclature : cathepsin Z
GENEID : 1522
GI : 61366435
GenBank Accession : AY890922
HGNC : 2547
MIM : 603169
Vega : OTTHUMG00000032858
Target GenBank: NM_001336

 

Reference Sequence Alignment

  

HsCD00000629      ATGGCGAGGCGCGGGCCAGGGTGGCGGCCGCTTCTGCTGCTCGTGCTGCTGGCGGGCGCG
NM_001336.3       ATGGCGAGGCGCGGGCCAGGGTGGCGGCCGCTTCTGCTGCTCGTGCTGCTGGCGGGCGCG
                  ************************************************************

HsCD00000629      GCGCAGGGCGGCCTCTACTTCCGCCGGGGACAGACCTGCTACCGGCCTCTGCGGGGGGAC
NM_001336.3       GCGCAGGGCGGCCTCTACTTCCGCCGGGGACAGACCTGCTACCGGCCTCTGCGGGGGGAC
                  ************************************************************

HsCD00000629      GGGCTGGCTCCGCTGGGGCGCAGCACATACCCCCGGCCTCATGAGTACCTGTCCCCAGCG
NM_001336.3       GGGCTGGCTCCGCTGGGGCGCAGCACATACCCCCGGCCTCATGAGTACCTGTCCCCAGCG
                  ************************************************************

HsCD00000629      GATCTGCCCAAGAGCTGGGACTGGCGCAATGTGGATGGTGTCAACTATGCCAGCATCACC
NM_001336.3       GATCTGCCCAAGAGCTGGGACTGGCGCAATGTGGATGGTGTCAACTATGCCAGCATCACC
                  ************************************************************

HsCD00000629      CGGAACCAGCACATCCCCCAATACTGCGGCTCCTGCTGGGCCCACGCCAGCACCAGCGCT
NM_001336.3       CGGAACCAGCACATCCCCCAATACTGCGGCTCCTGCTGGGCCCACGCCAGCACCAGCGCT
                  ************************************************************

HsCD00000629      ATGGCGGATCGGATCAACATCAAGAGGAAGGGAGCGTGGCCCTCCACCCTCCTGTCCGTG
NM_001336.3       ATGGCGGATCGGATCAACATCAAGAGGAAGGGAGCGTGGCCCTCCACCCTCCTGTCCGTG
                  ************************************************************

HsCD00000629      CAGAACGTCATCGACTGCGGTAACGCTGGCTCCTGTGAAGGGGGTAATGACCTGTCCGTG
NM_001336.3       CAGAACGTCATCGACTGCGGTAACGCTGGCTCCTGTGAAGGGGGTAATGACCTGTCCGTG
                  ************************************************************

HsCD00000629      TGGGACTACGCCCACCAGCACGGCATCCCTGACGAGACCTGCAACAACTACCAGGCCAAG
NM_001336.3       TGGGACTACGCCCACCAGCACGGCATCCCTGACGAGACCTGCAACAACTACCAGGCCAAG
                  ************************************************************

HsCD00000629      GACCAGGAGTGTGACAAGTTTAACCAATGTGGGACATGCAATGAATTCAAAGAGTGCCAC
NM_001336.3       GACCAGGAGTGTGACAAGTTTAACCAATGTGGGACATGCAATGAATTCAAAGAGTGCCAC
                  ************************************************************

HsCD00000629      GCCATCCGGAACTACACCCTCTGGAGGGTGGGAGACTACGGCTCCCTCTCTGGGAGGGAG
NM_001336.3       GCCATCCGGAACTACACCCTCTGGAGGGTGGGAGACTACGGCTCCCTCTCTGGGAGGGAG
                  ************************************************************

HsCD00000629      AAGATGATGGCAGAAATCTACGCAAATGGTCCCATCAGCTGTGGAATAATGGCAACAGAA
NM_001336.3       AAGATGATGGCAGAAATCTACGCAAATGGTCCCATCAGCTGTGGAATAATGGCAACAGAA
                  ************************************************************

HsCD00000629      AGACTGGCTAACTACACCGGAGGCATCTATGCCGAATACCAGGACACCACATATATAAAC
NM_001336.3       AGACTGGCTAACTACACCGGAGGCATCTATGCCGAATACCAGGACACCACATATATAAAC
                  ************************************************************

HsCD00000629      CATGTCGTTTCCGTGGCTGGGTGGGGCATCAGTGATGGGACTGAGTACTGGATTGTCCGG
NM_001336.3       CATGTCGTTTCTGTGGCTGGGTGGGGCATCAGTGATGGGACTGAGTACTGGATTGTCCGG
                  *********** ************************************************

HsCD00000629      AATTCATGGGGTGAACCATGGGGCGAGAGAGGCTGGCTGAGGATCGTGACCAGCACCTAT
NM_001336.3       AATTCATGGGGTGAACCATGGGGCGAGAGAGGCTGGCTGAGGATCGTGACCAGCACCTAT
                  ************************************************************

HsCD00000629      AAGGATGGGAAGGGCGCCAGATACAACCTTGCCATCGAGGAGCACTGTACATTTGGGGAC
NM_001336.3       AAGGATGGGAAGGGCGCCAGATACAACCTTGCCATCGAGGAGCACTGTACATTTGGGGAC
                  ************************************************************

HsCD00000629      CCCATCGTTTTG
NM_001336.3       CCCATCGTTTAA
                  **********:.

Pathways

Reactome : Clathrin derived vesicle budding
Reactome : Lysosome Vesicle Biogenesis
Reactome : Membrane Trafficking
Reactome : Metabolism of Angiotensinogen to Angiotensins
Reactome : Metabolism of proteins
Reactome : Peptide hormone metabolism
Reactome : trans-Golgi Network Vesicle Budding

Protein

SMART domain : Pept_C1 : Papain family cysteine protease
UniProt : Q9UBR2
HPRD : 04410

Clone

Cloning Information : 20128
HIP Master Clone ID : 36224
Original Clone ID : FLH020128.01L

Organism


TAX_ID : 9606
Species Specific ID: 1522

Genome

Chromosome : 20
Map Location : 20q13.32
Ensembl : ENSG00000101160

Reagents

Labome : CTSZ-antibody