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Detailed Vector Information: pRSET-natTEVGFPHis

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pRSET-natTEVGFPHis              
Source: Center for Membrane Proteins of Infectious Diseases
Description: Bacterial expression vector with T7 promoter and C-terminal TEV-cleavavable GFP and 6xHis-tags; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: None
Publications: None
Authors: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
EvNO00629004

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pRSET-natTEVGFPHis

pRSET-natTEVGFPHis in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  GFPR
Description: Bacterial expression vector with T7 promoter and C-terminal TEV-cleavavable GFP and 6xHis-tags; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Size (bp): 4016
Parent Vector: None
Properties: bacterial expression, fluorescent marker, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3166 3839
promoter T7 T7 promoter 20 39
protease cleavage site TEV TEV protease cleavage site 532 549
ribosome binding site RBS ribosome binding site 85 92
selectable marker AmpR ampicillin resistance 2161 3021
ssDNA origin f1 f1 origin 1575 2030
tag GFP C-terminal GFP 586 1299
tag His C-terminal 6xHis tag 1303 1320
trxn termination sequence T7 term T7 terminator 1375 1504

 

Species Specific ID: None
Target GenBank: None