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Detailed Vector Information: pCMVi-LSrCOMPTT

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pCMVi-LSrCOMPTT              
Source: Center for Membrane Proteins of Infectious Diseases
Description: with tag/fusion/marker assay, ligation independent cloning (LIC) clone, mammalian expression expression with intron and CMV and oligomerization domain and hGH and SV40 and leader sequence and AmpR and f1 and ColE and peptide and -10/Spec-R and COMP and Tetanus Toxoid and epitope and Stop Codons and InTron For and 013_DO3_DTH21 and RBS and 014_C03_DTH22 and 2012 and HGHTER and CMV-F; ampicillin resistance in bacterial;
Comments: None
Publications: None
Authors: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
EvNO00629005

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No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pCMVi-LSrCOMPTT

pCMVi-LSrCOMPTT in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  TTFOR
Reverse:  HGHTER
Description: with tag/fusion/marker assay, ligation independent cloning (LIC) clone, mammalian expression expression with intron and CMV and oligomerization domain and hGH and SV40 and leader sequence and AmpR and f1 and ColE and peptide and -10/Spec-R and COMP and Tetanus Toxoid and epitope and Stop Codons and InTron For and 013_DO3_DTH21 and RBS and 014_C03_DTH22 and 2012 and HGHTER and CMV-F; ampicillin resistance in bacterial;
Comments: Clone insert in-frame using the BglII restriction sites and another downstream site before the transcription terminator (such as XmaI)
Size (bp): 5476
Parent Vector: None
Properties: ligation independent cloning (LIC), mammalian expression, with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
-10 signal -10/Spec-R 0 0
3' Clip 014_C03_DTH22 735 1631
3' Clip 013_DO3_DTH21 774 1615
3' Clip Stop Codons 1625 1630
CDS COMP 1244 1378
Origin SV40 2316 2622
bacterial origin ColE ColE1 origin 2982 3839
binding site RBS 1070 1082
domain oligomerization domain N-terminal pentamerizing COMP domain from rat matrix protein 1223 1378
intron intron intron 861 993
leader sequence leader sequence secretion leader sequence from human alpha1-antitrypsin 1079 1150
peptide Tetanus Toxoid P30 1406 1450
peptide peptide N-terminal highly immunogenic 20-mer random peptide 1160 1219
peptide epitope N-terminal tetanus toxin epitopes to overcome humoral tolerance 1478 1546
primer HGHTER 1678 1698
primer CMV-F 672 692
primer 2012 1679 1698
primer InTron For 976 994
promoter CMV CMV promoter 1 744
selectable marker AmpR ampicillin resistance 3849 4758
ssDNA origin f1 f1 origin 4841 5285
trxn termination sequence hGH hGH terminator 1640 2116

 

Species Specific ID: None
Target GenBank: None