DNASU Plasmid Repository • 480.965.5697 | Email

Detailed Vector Information: pBAD24sigNtermHis

TOP

Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pBAD24sigNtermHis              
Source: Center for Membrane Proteins of Infectious Diseases
Description: Arabinose-inducible bacterial expression with a pBAD promoter and N-terminal E. coli YtfM (aa1-21) and 6xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: None
Publications: None
Authors: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
EvNO00629007

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pBAD24sigNtermHis

pBAD24sigNtermHis in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  PBADFOR
Reverse:  RRNBTER
Description: Arabinose-inducible bacterial expression with a pBAD promoter and N-terminal E. coli YtfM (aa1-21) and 6xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: Signal sequence is aa 1-21 from E. coli YtfM, which was supposedly used in PMID 20060837. Unfortunately, the actual sequence used in this publication was actually YtfM aa 1-27 with the V20A mutation, followed by 6xHis + LE (van den Berg, personal communication)
Size (bp): 5013
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 origin 3719 4416
promoter pBAD pBad promoter 1250 1277
repressor binding site arabinose O2 operator arabinose O2 operator 1003 1020
repressor binding site arabinose O1 operator arabinose O1 operator 1161 1182
repressor protein gene araC araC 96 974
ribosome binding site RBS ribosome binding site 1306 1311
selectable marker AmpR ampicillin resistance 2356 3219
signal sequence YtfM N-terminal E. coli YtfM (aa1-21) signal sequence 1320 1382
tag His N-terminal 6xHis tag 1383 1400
trxn termination sequence rrnB rrnB transcription terminsator 1838 2263

 

Species Specific ID: None
Target GenBank: None