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Detailed Vector Information: pRSET-YtfM-8xHis-N-term

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pRSET-YtfM-8xHis-N-term              
Source: Center for Membrane Proteins of Infectious Diseases
Description: Bacterial expression with T7 promoter and N-terminal E. coli YtfM (aa1-21) signal sequence and 8xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: None
Publications: None
Authors: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
EvNO00629009

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pRSET-YtfM-8xHis-N-term

pRSET-YtfM-8xHis-N-term in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression with T7 promoter and N-terminal E. coli YtfM (aa1-21) signal sequence and 8xHis-tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: Signal sequence is aa 1-21 from E. coli YtfM, which was supposedly used in PMID 20060837. Unfortunately, the actual sequence used in this publication was actually YtfM aa 1-27 with the V20A mutation, followed by 6xHis + LE (van den Berg, personal communication)
Size (bp): 3311
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 2461 3134
promoter T7 T7 promoter 20 39
ribosome binding site RBS ribosome binding site 85 92
selectable marker AmpR ampicillin resistance 1456 2316
signal sequence YtfM N-terminal E. coli YtfM (aa1-21) signal sequence 100 162
ssDNA origin f1 f1 origin 870 1325
tag His N-terminal 8xHis tag 163 186
trxn termination sequence T7 term T7 terminator 670 799

 

Species Specific ID: None
Target GenBank: None