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Detailed Vector Information: pRSET-C6xHis

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pRSET-C6xHis              
Source: Center for Membrane Proteins of Infectious Diseases
Description: Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: None
Publications: None
Authors: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
EvNO00629011

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Special MTA: PSI

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pRSET-C6xHis

pRSET-C6xHis in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and C-terminal 6xHis tag; ampicillin resistance in bacteria; ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Comments: ligation-independent (e.g., Clontech InFusion) following digestion with BseRI
Size (bp): 3242
Parent Vector: None
Properties: bacterial expression, ligation independent cloning (LIC), with tag/fusion/marker
Author Name: PSI:Biology
Arizona State University
Center for Membrane Proteins in Infectious Diseases
Felicia M. Craciunescu
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 2342 3024
promoter T7 T7 promoter 20 39
ribosome binding site RBS Ribosome binding site 85 92
selectable marker AmpR ampicillin resistance 1387 2247
ssDNA origin f1 f1 origin 801 1256
tag His C-terminal 6xHis tag 532 549
trxn termination sequence T7 term T7 terminator 601 730

 

Species Specific ID: None
Target GenBank: None