DNASU Plasmid Repository • 480.965.5697 | Email

CYP3A43 (Homo sapiens) in pANT7_cGST (GST-tagged in vitro expression vector)

TOP

Explanation of Terms


Gene: Gene Symbol:  CYP3A43
Gene Name:  cytochrome P450, family 3, subfamily A, polypeptide 43
Sequence             
Original Clone ID: None
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pANT7_cGST               Format:  FUSION
Source: Center for Personalized Diagnostics
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Center for Personalized Diagnostics
HsCD00629505

Price:  Login for Pricing
AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1179nts         Open reading frame : 1 to 1179

ATGGATCTCATTCCAAACTTTGCCATGGAAACATGGGTTCTTGTGGCTACCAGCCTGGTA
CTCCTCTATATTTATGGGACCCATTCACATAAACTTTTTAAGAAGCTGGGAATTCCTGGG
CCAACCCCTCTGCCTTTTCTGGGAACTATTTTGTTCTACCTTAGGAGGTCTCTGAATAAG
ATTCCCAGCTGGGCGTGGTGGCTCACGCCTGTAATCCCAGCACTTTGGGAGGCTGAAGCA
GGAGGATCACCTAAGGTCAGGAGTTCGAGACCAGCTTTGCCAACATGGGTCTTTGGAATT
TTGACAGAGAATGTAATGAAAAATACGGAGAAATGTGGGGCACTCTTTCCATTTCTTACC
CCAGTTTTTGAAGCCCTAAATATCGGTTTGTTTCCAAAAGATGTTACCCATTTTTTAAAA
AATTCCATTGAAAGGATGAAAGAAAGTCGCCTCAAAGATAAACAAAAGCATCGAGTAGAT
TTCTTTCAACAGATGATCGACTCCCAGAATTCCAAAGAAACAAAGTCCCATAAAGCTCTG
TCTGATCTGGAGCTTGTGGCCCAGTCAATTATCATCATTTTTGCTGCCTATGACACAACT
AGCACCACTCTCCCCTTCATTATGTATGAACTGGCCACTCACCCTGATGTCCAGCAGAAA
CTGCAGGAGGAGATTGACGCAGTTTTACCCAATAAGGCACCTGTCACCTACGATGCCCTG
GTACAGATGGAGTACCTTGACATGGTGGTGAATGAAACGCTCAGATTATTCCCAGTTGTT
AGTAGAGTTACGAGAGTCTGCAAGAAAGATATTGAAATCAATGGAGTGTTCATTCCCAAA
GGGTTAGCAGTGATGGTTCCAATCTATGCTCTTCACCATGACCCAAAGTACTGGACAGAG
CCTGAGAAGTTCTGCCCTGAAAGGTTCAGTAAGAAGAACAAGGACAGCATAGATCTTTAC
AGATACATACCTTTTGGAGCTGGACCCCGAAACTGCATTGGCATGAGGTTTGCTCTCACA
AACATAAAACTTGCTGTCATTAGAGCACTGCAGAACTTCTCCTTCAAACCTTGTAAAGAG
ACTCAGATCCCACTGAAATTAGACAATCTACCAATTCTTCAACCAGAAAAACCTATTGTT
CTAAAAGTGCACTTAAGAGATGGGATTACAAGTGGACCC


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

         Powered by LabGenius

Vector Name: pANT7_cGST

pANT7_cGST in advanced viewed

Synonyms: pANT7-cGST
Sequencing Primer: Forward:  NAP150
Reverse:  NAP138R
Description: with tag/fusion/marker assay, Gateway and reg, acceptor (destination), recombinational cloning clone, in vitro transcription expression with attR1 and NAP150 and ccdB and attR2 and GST and CmR and T7 and NAP138 and ColE and AmpR ampicillin resistance in bacterial
Comments: One in a series of pANT7 vectors made by A. Lau and N. Ramachandran for use with NAPPA arrays. Can be used for cell free expression of the gene insert using human IVTT from Thermo Fisher or rabbit reticulocyte systems
Size (bp): 5964
Parent Vector: None
Empty Vector: EvNO00023103
Properties: Gateway, acceptor (destination), in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Al Lau
Niroshan Ramachandran
Joshua LaBaer
Publications: PMID: 15232106
Title: Self-assembling protein microarrays

Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin of replication 3931 3249
death cassette ccdB ccdB death cassette present in 'empty vector' form only (replace with gene of interest) 1876 2085
primer NAP138 NAP138 reverse sequencing primer 5? TGTTTCGCCATTTATCACCTTC 3? 2405 2384
primer NAP150 Forward sequencing primer NAP150 5?-CCC ATT GTA TGG GAT CTG ATC-3? 388 408
promoter T7 T7 transcriptional start sequence 5940 5964
recombination site attR2 AttR2 site for recombination in empty vector 2236 2251
recombination site attR1 AttR1 site for recombination in empty vector 549 565
selectable marker CmR chloramphenicol resistance 782 1438
selectable marker AmpR ampicillin resistance gene 4688 4029
tag GST Glutathione transferase (GST) tag 2282 2950

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome

Gene

 

Gene Symbol : CYP3A43
Symbol Nomenclature : CYP3A43
Designation : cytochrome P450 3A43|cytochrome P450, subfamily IIIA, polypeptide 43
Full Nomenclature : cytochrome P450, family 3, subfamily A, polypeptide 43
GENEID : 64816
HGNC : 17450
MIM : 606534
Vega : OTTHUMG00000156498
Target GenBank: None

 

Pathways

Reactome : Biological oxidations
Reactome : Cytochrome P450 - arranged by substrate type
Reactome : Metabolism
Reactome : Miscellaneous substrates
Reactome : Phase 1 - Functionalization of compounds
Reactome : Xenobiotics
WikiPathway : cytochrome P450
WikiPathway : metapathway biotransformation

Protein

HPRD : 05942

Clone

Cloning Information : 676471

Organism


TAX_ID : 9606
Species Specific ID: 64816

Genome

Chromosome : 7
Map Location : 7q21.1
Ensembl : ENSG00000021461