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nuoF (Francisella tularensis Schu 4) in pDEST17 (bacterial expression vector)

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Explanation of Terms


Gene: Gene Symbol:  nuoF
Gene Name:  NADH dehydrogenase I, F subunit
Sequence             
Original Clone ID: FLH233290.01X
Keyword: None
Species: Francisella tularensis Schu 4
Type: cDNA
Vector Name: pDEST17               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors:
FtCD00063391

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1275nts         Open reading frame : 1 to 1275

ATGGCAAATGAAGTTTGTTTTCGTACTCTACATTTAGATAAGCCATACAGTTTGGAGTCT
TATTTATCTGTGGGAGGGTATTCCTACTGGAATAAAATATTGACAGAAAAAATTCCACCT
GAGCAAATTATTGAAGAGCTCAAAATATCTGGGCTTAGGGGTCGTGGTGGTGCTGGTTTC
CCTACGGGATTGAAATGGAGTTTTATGCCACGTAATGCTCCGGGGCAGAAATACGTTGTA
TGCAACTCTGATGAGGGTGAGCCTGGTACATGTAAGGATAGAGAAATTCTTAGGAATAAT
CCTCATCAATTGATTGAAGGTATAGCTATTGCTGGATATGTTGTTGGGGCTACTGCAGGC
TATAACTATACTAGAGGCGAGTTCTATGAGCCAATAGCTAGGTTTGAAGACGCGCTTATT
GAGGCATATCAAGCAGGTTTATTGGGTACAAATATCAAATCATCAGGAATCTCTTTTGAT
TTATACTCAGCTATTGGTGCTGGTGCTTATATCTGTGGTGAAGAAACAGCATTGCTTAAC
TCACTTGAGGGTAAAAAAGGTCGCCCAAGATTTAAGCCACCATTTCCTGCATTTAAAGGT
CTTTATGACAAGCCAACAAATATAAATAATACAGAAACATATGCTTCAGTGCCGGCAATA
TTACAATATGGCGGTGAGTGGTTTAAGAAATTGGGTACTGAGAAAAGTGGCGGTACTAAG
TTATTCTGTGTCTCTGGACATGTACAGCAGCAAAGAGTTGTTGAAATAGGTTTAGGTATG
CCGTTTAAAGAGCTTTTAGACATGTGCGGAGGCGTTCGCAATGGTAATAAGCTTAAGGCA
GTAATCCCTGGTGGCAGCTCATCTAAGATCTTGACAGCTGATGAGATGATGGCTGTTACT
ATGGATTATGAGTCAATAGCAGCGGCAGGTTCAATGCTTGGTTCAGGAGCAGTTGTAGTG
TTGGATGAGACTACATGTATTGTTAGAACTCTAGCAAGGCTTGCTGATTTCTATTATGAA
GAGTCTTGTGGTCAATGTACGCCTTGTAGAGAGGGTACTGGGTGGTTAGCACGAACTTTA
CATAGAATTGTTGCTGGTGAAGGAAGACCGGAGGATATTGATACTTTAGTTAGAGTAGCA
ACAAATATAGAGGGTAATACTATTTGTGGTTTGGGTGATGCAGCTGCATGGCCAGTCCAG
AGTTATATAGATAAATTTAGGCATGAGTTTGTTTATATGATCGAGAATAATGGTAGAAGT
ATAGTGGATCAATAG


Coding Sequence Details
Insert Sequence Verified?: N Verification Method: Insert was fully sequenced in parent vector
Reference Sequence Annotations:
End on reference sequence 1275
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDEST17

pDEST17 in advanced viewed

Synonyms: pDEST-17
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 promoter and ribosome binding site (RBS), adds N-term 6xHis tag; amp resistance; recombinational cloning.
Comments: None
Size (bp): 6354
Parent Vector: None
Empty Vector: None
Properties: Gateway, acceptor (destination), bacterial expression, in vitro transcription, recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
ATG start ATG initiation ATG (before 6xHis tag) 101 103
bacterial origin pBR322 ori pBR322 origin of replication 3575 4248
gene fragment ROP ROP ORF 4619 4810
negative selection marker ccdB ccdB death cassette ONLY in Non-Recombined (NO INSERT) form 1374 1679
promoter bla bla promoter (prokaryotic promoter from bla gene) 2471 2569
promoter T7 T7 promoter 21 40
recombination site attR1 attR1 recombination site 140 264
recombination site attR2 attR2 recombination site 1720 1844
ribosome binding site RBS Ribosome binding site 86 92
selectable marker CmR chlorampenicol resistance gene ONLY In Non-Recombined (NO INSERT) form 373 1032
selectable marker AmpR ampicillin resistance gene (bla) 2570 3430
tag His 6xHis tag (N-terminal to insert) 113 130
trxn termination sequence T7 term T7 transcription termination sequence 1855 1983

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Reagents

Gene

Target GenBank: YP_169112

 

Clone

Cloning Information : 233290
HIP Master Clone ID : 126854
Original Clone ID : FLH233290.01X

Organism


Species Specific ID: FTT0036

Reagents

Labome : nuoF-cDNA