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GTF2F2 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  GTF2F2
Gene Name:  general transcription factor IIF, polypeptide 2, 30kDa
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH008652.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000638

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 750nts         Open reading frame : 1 to 750
Reference Sequence Alignment

ATGGCCGAGCGCGGGGAACTCGACTTGACCGGCGCCAAACAGAACACAGGAGTGTGGCTA
GTCAAGGTTCCTAAATATTTGTCACAGCAATGGGCTAAAGCCTCTGGAAGAGGTGAAGTT
GGGAAACTGCGGATTGCCAAGACTCAAGGAAGGACTGAGGTGTCATTTACTTTGAATGAG
GATCTTGCAAATATTCATGATATTGGTGGAAAACCAGCTTCAGTCAGTGCTCCTAGAGAA
CATCCATTTGTCTTGCAAAGTGTTGGAGGACAGACATTAACAGTATTTACTGAGAGCTCA
TCAGATAAGCTGTCATTGGAAGGAATAGTGGTACAAAGAGCTGAATGCCGACCAGCTGCC
AGTGAAAACTACATGCGATTAAAAAGATTGCAAATAGAAGAGTCTTCCAAACCAGTGAGG
CTATCACAACAGCTGGACAAAGTTGTAACAACCAATTACAAACCTGTTGCTAATCATCAA
TACAATATCGAATATGAAAGGAAAAAGAAAGAAGACGGAAAGCGAGCTCGAGCTGATAAA
CAACATGTTTTAGACATGCTATTTTCAGCCTTTGAGAAACATCAATACTATAATCTTAAG
GACTTGGTGGACATCACAAAGCAACCTGTGGTGTACCTGAAGGAAATCTTAAAAGAAATT
GGTGTTCAGAATGTAAAAGGGATCCACAAAAACACATGGGAGCTGAAGCCAGAGTACAGA
CACTATCAAGGAGAAGAAAAGAGTGACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 869
Start on reference sequence 120
Mutations:
None
Discrepancy:
a621g
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : GTF2F2
Symbol Nomenclature : GTF2F2
SYNONYM : BTF4; RAP30; TF2F2; TFIIF
Designation : ATP-dependent helicase GTF2F2|TFIIF-beta|general transcription factor IIF 30 kDa subunit|general transcription factor IIF subunit 2|transcription initiation factor IIF subunit beta|transcription initiation factor RAP30
Full Nomenclature : general transcription factor IIF, polypeptide 2, 30kDa
GENEID : 2963
GI : 61356696
GenBank Accession : AY888329
HGNC : 4653
MIM : 189969
Vega : OTTHUMG00000016849
Target GenBank: NM_004128

 

Reference Sequence Alignment

  

HsCD00000638      ATGGCCGAGCGCGGGGAACTCGACTTGACCGGCGCCAAACAGAACACAGGAGTGTGGCTA
NM_004128.2       ATGGCCGAGCGCGGGGAACTCGACTTGACCGGCGCCAAACAGAACACAGGAGTGTGGCTA
                  ************************************************************

HsCD00000638      GTCAAGGTTCCTAAATATTTGTCACAGCAATGGGCTAAAGCCTCTGGAAGAGGTGAAGTT
NM_004128.2       GTCAAGGTTCCTAAATATTTGTCACAGCAATGGGCTAAAGCCTCTGGAAGAGGTGAAGTT
                  ************************************************************

HsCD00000638      GGGAAACTGCGGATTGCCAAGACTCAAGGAAGGACTGAGGTGTCATTTACTTTGAATGAG
NM_004128.2       GGGAAACTGCGGATTGCCAAGACTCAAGGAAGGACTGAGGTGTCATTTACTTTGAATGAG
                  ************************************************************

HsCD00000638      GATCTTGCAAATATTCATGATATTGGTGGAAAACCAGCTTCAGTCAGTGCTCCTAGAGAA
NM_004128.2       GATCTTGCAAATATTCATGATATTGGTGGAAAACCAGCTTCAGTCAGTGCTCCTAGAGAA
                  ************************************************************

HsCD00000638      CATCCATTTGTCTTGCAAAGTGTTGGAGGACAGACATTAACAGTATTTACTGAGAGCTCA
NM_004128.2       CATCCATTTGTCTTGCAAAGTGTTGGAGGACAGACATTAACAGTATTTACTGAGAGCTCA
                  ************************************************************

HsCD00000638      TCAGATAAGCTGTCATTGGAAGGAATAGTGGTACAAAGAGCTGAATGCCGACCAGCTGCC
NM_004128.2       TCAGATAAGCTGTCATTGGAAGGAATAGTGGTACAAAGAGCTGAATGCCGACCAGCTGCC
                  ************************************************************

HsCD00000638      AGTGAAAACTACATGCGATTAAAAAGATTGCAAATAGAAGAGTCTTCCAAACCAGTGAGG
NM_004128.2       AGTGAAAACTACATGCGATTAAAAAGATTGCAAATAGAAGAGTCTTCCAAACCAGTGAGG
                  ************************************************************

HsCD00000638      CTATCACAACAGCTGGACAAAGTTGTAACAACCAATTACAAACCTGTTGCTAATCATCAA
NM_004128.2       CTATCACAACAGCTGGACAAAGTTGTAACAACCAATTACAAACCTGTTGCTAATCATCAA
                  ************************************************************

HsCD00000638      TACAATATCGAATATGAAAGGAAAAAGAAAGAAGACGGAAAGCGAGCTCGAGCTGATAAA
NM_004128.2       TACAATATCGAATATGAAAGGAAAAAGAAAGAAGACGGAAAGCGAGCTCGAGCTGATAAA
                  ************************************************************

HsCD00000638      CAACATGTTTTAGACATGCTATTTTCAGCCTTTGAGAAACATCAATACTATAATCTTAAG
NM_004128.2       CAACATGTTTTAGACATGCTATTTTCAGCCTTTGAGAAACATCAATACTATAATCTTAAG
                  ************************************************************

HsCD00000638      GACTTGGTGGACATCACAAAGCAACCTGTGGTGTACCTGAAGGAAATCTTAAAAGAAATT
NM_004128.2       GACTTGGTGGACATCACAAAGCAACCTGTGGTGTACCTGAAGGAAATCTTAAAAGAAATT
                  ************************************************************

HsCD00000638      GGTGTTCAGAATGTAAAAGGGATCCACAAAAACACATGGGAGCTGAAGCCAGAGTACAGA
NM_004128.2       GGTGTTCAGAATGTAAAAGGGATCCACAAAAACACATGGGAGCTGAAGCCAGAGTACAGA
                  ************************************************************

HsCD00000638      CACTATCAAGGAGAAGAAAAGAGTGACTAG
NM_004128.2       CACTATCAAGGAGAAGAAAAGAGTGACTAA
                  *****************************.

Pathways

Panther : General transcription regulation
Panther : Transcription regulation by bZIP transcription factor
Reactome : Abortive elongation of HIV-1 transcript in the absence of Tat
Reactome : Disease
Reactome : Elongation arrest and recovery
Reactome : Formation of HIV elongation complex in the absence of HIV Tat
Reactome : Formation of HIV-1 elongation complex containing HIV-1 Tat
Reactome : Formation of RNA Pol II elongation complex
Reactome : Formation of the Early Elongation Complex
Reactome : Formation of the HIV-1 Early Elongation Complex
Reactome : Gene Expression
Reactome : HIV Infection
Reactome : HIV Life Cycle
Reactome : HIV Transcription Elongation
Reactome : HIV Transcription Initiation
Reactome : HIV elongation arrest and recovery
Reactome : Influenza Infection
Reactome : Influenza Life Cycle
Reactome : Influenza Viral RNA Transcription and Replication
Reactome : Late Phase of HIV Life Cycle
Reactome : Pausing and recovery of HIV elongation
Reactome : Pausing and recovery of Tat-mediated HIV elongation
Reactome : Processing of Capped Intron-Containing Pre-mRNA
Reactome : RNA Pol II CTD phosphorylation and interaction with CE
Reactome : RNA Polymerase II HIV Promoter Escape
Reactome : RNA Polymerase II Pre-transcription Events
Reactome : RNA Polymerase II Promoter Escape
Reactome : RNA Polymerase II Transcription
Reactome : RNA Polymerase II Transcription Elongation
Reactome : RNA Polymerase II Transcription Initiation
Reactome : RNA Polymerase II Transcription Initiation And Promoter Clearance
Reactome : RNA Polymerase II Transcription Pre-Initiation And Promoter Opening
Reactome : Tat-mediated HIV elongation arrest and recovery
Reactome : Tat-mediated elongation of the HIV-1 transcript
Reactome : Transcription
Reactome : Transcription of the HIV genome
Reactome : Viral Messenger RNA Synthesis
Reactome : mRNA Capping
Reactome : mRNA Splicing
Reactome : mRNA Splicing - Major Pathway
Reactome : mRNA Splicing - Minor Pathway
WikiPathway : Eukaryotic Transcription Initiation

Protein

UniProt : P13984
HPRD : 01804

Clone

Cloning Information : 8652
HIP Master Clone ID : 11147
Original Clone ID : FLH008652.01X

Organism


TAX_ID : 9606
Species Specific ID: 2963

Genome

Chromosome : 13
Map Location : 13q14
Ensembl : ENSG00000188342

Reagents

Labome : GTF2F2-antibody