Gene:
Gene Symbol:
CASP8AP2
Gene Name:
caspase 8 associated protein 2
Original Clone ID:
HR8150A-1916-1982-Av6HT.3
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15Avi6HT_NESG
Format:
CLOSED
Source:
Northeast Structural Genomics Consortium
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
PMID: 24941917
Title: Solution NMR structures of homeodomains from human proteins ALX4, ZHX1, and CASP8AP2 contribute to the structural coverage of the Human Cancer Protein Interaction Network
Title: Solution NMR structures of homeodomains from human proteins ALX4, ZHX1, and CASP8AP2 contribute to the structural coverage of the Human Cancer Protein Interaction Network
Authors:
Rutgers University
PSI:Biology
Northeast Structural Genomics Consortium
PSI:Biology
Northeast Structural Genomics Consortium
HsCD00650855
Price:
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AVAILABLE
Academic and non-profit labs
Special MTA: PSI/NESG
Special MTA: PSI/NESG
Insert sequence: 204nts
Open reading frame: 10 to 213
AGCCATATGAAAAATGTTATTAAAAAGAAGGGAGAAATTATCATTTTATGGACAAGAAAT
GATGACCGGGTAATTTTATTGGAGTGTCAGAAAAGAGGGCCATCATCTAAAACATTTGCA
TATTTAGCCGCCAAGTTGGATAAAAATCCAAATCAGGTCTCAGAAAGATTCCAGCAGCTA
ATGAAGCTCTTTGAAAAGTCAAAATGCAGATAG
Protein sequence predicted based on nucleotide sequence
KNVIKKKGEIIILWTRNDDRVILLECQKRGPSSKTFAYLAAKLDKNPNQVSERFQQLMKL FEKSKCR*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
ProteinExpressed: Protein_Confirmed
ProteinPurified: Protein_Purified
SolubleProtein: Protein_Soluble
Find experimental protocols in HR8150A
Recommended expression in:
Not Applicable
Vector Name:
pET15Avi6HT_NESG
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 lac promoter, N-terminal TEV-cleavable Avi-tag and 6XHis-tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
None
Size (bp):
5789
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3944
4626
promoter
T7
T7 promoter
541
560
protease cleavage site
TEV
TEV protease cleavage site
387
404
repressor binding site
lac operator
Lac operator
519
541
repressor protein gene
LacI
Lac repressor
3344
3520
selectable marker
AmpR
ampicillin resistance
4724
5383
tag
His
N-terminal 6xHis tag
405
422
tag
AviTag
Avi Tag
423
466
trxn termination sequence
T7 term
T7 terminator
217
263
