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VDR (Homo sapiens) in pET15Avi6HT_NESG

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Explanation of Terms


Gene: Gene Symbol:  VDR
Gene Name:  vitamin D (1,25- dihydroxyvitamin D3) receptor
Sequence             
Original Clone ID: None
Keyword: targeted domain
Species: Homo sapiens
Type: cDNA
Vector Name: pET15Avi6HT_NESG               Format:  FUSION
Source: Rutgers University
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Stephen Anderson
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
HsCD00651882

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AVAILABLE
No restriction
Special MTA: SPTA

 

Insert sequence: 615nts        
Open reading frame : 1 to 615

TCCCAGCTCTCCATGCTGCCCCACCTGGCTGACCTGGTCAGTTACAGCATCCAAAAGGTC
ATTGGCTTTGCTAAGATGATACCAGGATTCAGAGACCTCACCTCTGAGGACCAGATCGTA
CTGCTGAAGTCAAGTGCCATTGAGGTCATCATGTTGCGCTCCAATGAGTCCTTCACCATG
GACGACATGTCCTGGACCTGTGGCAACCAAGACTACAAGTACCGCGTCAGTGACGTGACC
AAAGCCGGACACAGCCTGGAGCTGATTGAGCCCCTCATCAAGTTCCAGGTGGGACTGAAG
AAGCTGAACTTGCATGAGGAGGAGCATGTCCTGCTCATGGCCATCTGCATCGTCTCCCCA
GATCGTCCTGGGGTGCAGGACGCCGCGCTGATTGAGGCCATCCAGGACCGCCTGTCCAAC
ACACTGCAGACGTACATCCGCTGCCGCCACCCGCCCCCGGGCAGCCACCTGCTCTATGCC
AAGATGATCCAGAAGCTAGCCGACCTGCGCAGCCTCAATGAGGAGCACTCCAAGCAGTAC
CGCTGCCTCTCCTTCCAGCCTGAGTGCAGCATGAAGCTAACGCCCCTTGTGCTCGAAGTG
TTTGGCAATGAGTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pET15Avi6HT_NESG

pET15Avi6HT_NESG in advanced viewed

Synonyms: ''
Sequencing Primer: Forward:  T7
Reverse:  T7 terminator
Description: Bacterial expression vector with T7 lac promoter, N-terminal TEV-cleavable Avi-tag and 6XHis-tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments: None
Size (bp): 5789
Parent Vector: None
Empty Vector: None
Properties: bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name: Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 origin 3944 4626
promoter T7 T7 promoter 541 560
protease cleavage site TEV TEV protease cleavage site 387 404
repressor binding site lac operator Lac operator 519 541
repressor protein gene LacI Lac repressor 3344 3520
selectable marker AmpR ampicillin resistance 4724 5383
tag His N-terminal 6xHis tag 405 422
tag AviTag Avi Tag 423 466
trxn termination sequence T7 term T7 terminator 217 263

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Experimental
  • Organism
  • Genome

Gene

 

Gene Symbol : VDR
Symbol Nomenclature : VDR
SYNONYM : NR1I1
Designation : 1,25-dihydroxyvitamin D3 receptor|nuclear receptor subfamily 1 group I member 1|vitamin D nuclear receptor variant 1|vitamin D3 receptor
Full Nomenclature : vitamin D (1,25- dihydroxyvitamin D3) receptor
GENEID : 7421
HGNC : 12679
MIM : 601769
Vega : OTTHUMG00000169890
Target GenBank: None

 

Pathways

NCI : Direct p53 effectors
NCI : Regulation of nuclear SMAD2/3 signaling
NCI : Retinoic acid receptors-mediated signaling
NCI : RXR and RAR heterodimerization with other nuclear receptor
NCI : Validated transcriptional targets of deltaNp63 isoforms
NCI : Validated transcriptional targets of TAp63 isoforms
Panther : Vitamin D metabolism and pathway
Reactome : Gene Expression
Reactome : Generic Transcription Pathway
Reactome : Nuclear Receptor transcription pathway
WikiPathway : Nuclear Receptors
WikiPathway : Nuclear receptors in lipid metabolism and toxicity
WikiPathway : Ovarian Infertility Genes
WikiPathway : Vitamin D synthesis

Protein

Structural Annotation Wiki : HR7564D
UniProt : P11473
HPRD : 03463

Clone

Cloning Information : 678443

Experimental

TargetTrack - Experimental data : HR7564D

Organism


TAX_ID : 9606
Species Specific ID: 7421

Genome

Chromosome : 12
Map Location : 12q13.11
Ensembl : ENSG00000111424