Gene:
Gene Symbol:
NR0B1
Gene Name:
nuclear receptor subfamily 0, group B, member 1
Original Clone ID:
None
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15Avi6HT_NESG
Format:
FUSION
Source:
Rutgers University
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Stephen Anderson
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
HsCD00651891
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 705nts
Open reading frame: 1 to 705
GACACCTCCTCTGGTGCGCTGCGGCCGGTGGCGCTCAAGAGTCCACAGGTGGTCTGCGAG
GCAGCCTCAGCGGGCCTGTTGAAGACGCTGCGCTTCGTCAAGTACTTGCCCTGCTTCCAG
GTGCTGCCCCTGGACCAGCAGCTGGTGCTGGTGCGCAACTGCTGGGCGTCCCTGCTCATG
CTTGAGCTGGCCCAGGACCGCTTGCAGTTCGAGACTGTGGAAGTCTCGGAGCCCAGCATG
CTGCAGAAGATCCTCACCACCAGGCGGCGGGAGACCGGGGGCAACGAGCCACTGCCCGTG
CCCACGCTGCAGCACCATTTGGCACCGCCGGCGGAGGCCAGGAAGGTGCCCTCCGCCTCC
CAGGTCCAAGCCATCAAGTGCTTTCTTTCCAAATGCTGGAGTCTGAACATCAGTACCAAG
GAGTACGCCTACCTCAAGGGGACCGTGCTCTTTAACCCGGACGTGCCGGGCCTGCAGTGC
GTGAAGTACATTCAGGGACTCCAGTGGGGAACTCAGCAAATACTCAGTGAACACACCAGG
ATGACGCACCAAGGGCCCCATGACAGATTCATCGAACTTAATAGTACCCTTTTCCTGCTG
AGATTCATCAATGCCAATGTCATTGCTGAACTGTTCTTCAGGCCCATCATCGGCACAGTC
AGCATGGATGATATGATGCTGGAAATGCTCTGTACAAAGATATGA
Protein sequence predicted based on nucleotide sequence
DTSSGALRPVALKSPQVVCEAASAGLLKTLRFVKYLPCFQVLPLDQQLVLVRNCWASLLM LELAQDRLQFETVEVSEPSMLQKILTTRRRETGGNEPLPVPTLQHHLAPPAEARKVPSAS QVQAIKCFLSKCWSLNISTKEYAYLKGTVLFNPDVPGLQCVKYIQGLQWGTQQILSEHTR MTHQGPHDRFIELNSTLFLLRFINANVIAELFFRPIIGTVSMDDMMLEMLCTKI*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pET15Avi6HT_NESG
Synonyms:
''
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 lac promoter, N-terminal TEV-cleavable Avi-tag and 6XHis-tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
None
Size (bp):
5789
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Gaetano T. Montleione
Thomas B. Acton
Stephen Anderson
Northeast Structural Genomics Consortium
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 origin
3944
4626
promoter
T7
T7 promoter
541
560
protease cleavage site
TEV
TEV protease cleavage site
387
404
repressor binding site
lac operator
Lac operator
519
541
repressor protein gene
LacI
Lac repressor
3344
3520
selectable marker
AmpR
ampicillin resistance
4724
5383
tag
His
N-terminal 6xHis tag
405
422
tag
AviTag
Avi Tag
423
466
trxn termination sequence
T7 term
T7 terminator
217
263
