Gene:
Gene Symbol:
JARID2
Gene Name:
jumonji, AT rich interactive domain 2
Original Clone ID:
None
Keyword:
targeted domain
Species:
Homo sapiens
Type:
cDNA
Vector Name:
pET15-6HCBPT_TF
Format:
FUSION
Source:
Rutgers University
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Stephen Anderson
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
HsCD00651987
Price:
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AVAILABLE
No restriction
Special MTA: SPTA
Special MTA: SPTA
Insert sequence: 564nts
Open reading frame: 1 to 564
GGGTCCATCCTGCGTCACCTCGGTGCTGTGCCTGGAGTGACTATTCCCTGGCTAAATATT
GGCATGGTCTTTTCTACCTCATGCTGGTCTCGAGACCAAAATCACCTTCCATACATTGAC
TACTTACACACTGGTGCTGACTGCATTTGGTATTGCATTCCTGCTGAGGAGGAGAACAAG
CTGGAAGATGTGGTCCACACCCTGCTGCAAGCCAATGGCACCCCAGGGCTGCAGATGCTG
GAAAGCAACGTCATGATCTCCCCGGAGGTGCTGTGCAAAGAGGGGATCAAGGTGCACAGG
ACCGTGCAGCAGAGTGGCCAGTTTGTCGTCTGCTTCCCGGGATCCTTTGTGTCCAAAGTG
TGCTGTGGGTACAGCGTGTCTGAAACCGTGCACTTTGCTACCACCCAGTGGACAAGTATG
GGCTTTGAGACCGCCAAGGAAATGAAGCGTCGCCATATAGCTAAGCCATTCTCCATGGAG
AAGTTACTCTACCAGATTGCACAAGCAGAAGCAAAAAAAGAAAACGGTCCCACTCTCAGT
ACCATCTCAGCCCTCCTGGATTGA
Protein sequence predicted based on nucleotide sequence
GSILRHLGAVPGVTIPWLNIGMVFSTSCWSRDQNHLPYIDYLHTGADCIWYCIPAEEENK LEDVVHTLLQANGTPGLQMLESNVMISPEVLCKEGIKVHRTVQQSGQFVVCFPGSFVSKV CCGYSVSETVHFATTQWTSMGFETAKEMKRRHIAKPFSMEKLLYQIAQAEAKKENGPTLS TISALLD*
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
ampicillin
Bacterial Selection Condition:
100 ug/mL ampicillin
Growth Condition:
Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:
Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pET15-6HCBPT_TF
Synonyms:
6HCBPT
Sequencing Primer:
Forward:
T7
Reverse:
T7 terminator
Description:
Bacterial expression vector with T7 promoter and N-terminal TEV-cleavable 6xHis-CBP tags; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
pET15b-based (T7) N-terminal HisTag followed by calmodulin binding peptide and cleavable TEV linker
Size (bp):
5771
Parent Vector:
None
Properties:
bacterial expression, multiple cloning site, with tag/fusion/marker
Author Name:
Rutgers University
Gaetano T. Montleione
Northeast Structural Genomics Consortium
PSI:Biology
Gaetano T. Montleione
Northeast Structural Genomics Consortium
PSI:Biology
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
bacterial origin
ColE
ColE1 bacterial origin
1492
2174
gene fragment
ROP
repressor of protein
2598
2774
promoter
T7
T7 promoter
5558
5577
protease cleavage site
TEV
TEV protease cleavage site
5738
5758
repressor binding site
lac operator
lac operator
5577
5599
repressor protein gene
LacI
LacI
4089
5168
selectable marker
AmpR
ampicillin resistance
735
1394
tag
CBP
N-terminal calmodulin binding protein
5669
5737
tag
His
N-terminal 6xHis tag
5651
5668
trxn termination sequence
T7 term
T7 transcription terminator
70
116
