Detailed Plasmid Information

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name:  None
Original Clone ID:
None
Keyword:
None
Species:
Unknown
Type:
cDNA
Vector Name:
pET21_NESG
Format: 
FUSION
Source:
Rutgers University
Description:
None
Comments:
None
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Stephen Anderson
Elliot Campbell
Rutgers University
Protein Capture Reagents Program
HsCD00652007
Price: 
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AVAILABLE
No restriction
Special MTA: SPTA
Insert sequence: 384nts
Open reading frame: 1 to 384

ATGGGCGTGGTGATCCTGGTGCTGACCGGCGACGAACGTATTGCGGAAGAACTGCGTAAA
GAAGTCCAGAAGCATGACCCGAATGTGAAAACCGTTCCGACGAAGGATAAGGAAAAGGTC
AAAGAAGAAATCGAAAAGGCGCGTAAACAGGGCCGTCCGATCGTGGTTTTTGTCCGTGGC
GGTGATGACGAACGCGCCAAAGATATTGCGGAATATGCCCAGAAAGAAGGTCTGCGTGTC
ATCGTGATTATCGTGAGTCAAGACGAAGAAGCGCTGCGCAAAGGCTACGAAGATAAAAAG
AAAAAGGGTTACGACGTTTACACCAGCCGTAACGAAGATGAAGCAAAAAAGAAACTGAAG
GAAGCTCTGGAAAAAAGCGGCTCT

Protein sequence predicted based on nucleotide sequence

MGVVILVLTGDERIAEELRKEVQKHDPNVKTVPTKDKEKVKEEIEKARKQGRPIVVFVRG
GDDERAKDIAEYAQKEGLRVIVIIVSQDEEALRKGYEDKKKKGYDVYTSRNEDEAKKKLK
EALEKSGS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
Sequence Verification
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results:
None
Recommended expression in:
Not Applicable