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CYC1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CYC1
Gene Name:  cytochrome c-1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH008192.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000654

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 978nts         Open reading frame : 1 to 978
Reference Sequence Alignment

ATGGCGGCAGCTGCGGCTTCGCTTCGCGGGGTAGTGTTGGGCCCGCGGGGCGCGGGGCTC
CCGGGCGCGCGTGCCCGGGGTCTGCTGTGCAGCGCGCGGCCCGGGCAGCTCCCGCTACGG
ACACCTCAGGCAGTGGCCTTGTCGTCGAAGTCTGGCCTTTCCCGAGGCCGGAAAGTGATG
CTGTCAGCGCTGGGCATGCTGGCGGCAGGGGGTGCGGGGCTGGCCGTGGCTCTGCATTCG
GCTGTGAGTGCCAGTGACCTGGAGCTGCACCCCCCCAGCTATCCGTGGTCTCACCGTGGC
CTCCTCTCTTCCTTGGACCACACCAGCATCCGGAGGGGTTTCCAGGTATATAAGCAGGTG
TGCGCCTCCTGCCACAGCATGGACTTCGTGGCCTACCGCCACCTGGTGGGCGTGTGCTAC
ACGGAGGATGAAGCTAAGGAGCTGGCTGCGGAGGTGGAGGTTCAAGACGGCCCCAATGAA
GATGGGGAGATGTTCATGCGGCCAGGGAAGCTGTTCGACTATTTCCCAAAACCATACCCC
AACAGTGAGGCTGCTCGAGCTGCCAACAACGGAGCATTGCCCCCTGACCTCAGCTACATC
GTGCGAGCTAGGCATGGTGGTGAGGACTACGTCTTCTCCCTGCTCACGGGCTACTGCGAG
CCACCCACCGGGGTGTCACTGCGGGAAGGTCTCTACTTCAACCCCTACTTTCCTGGCCAG
GCCATTGCCATGGCCCCTCCCATCTACACAGATGTCTTAGAGTTTGACGATGGCACCCCA
GCTACCATGTCCCAGATAGCCAAGGATGTGTGCACCTTCCTGCGCTGGGCATCTGAGCCA
GAGCACGACCATCGAAAACGCATGGGGCTCAAGATGTTGATGATGATGGCTCTGCTGGTG
CCCCTGGTCTACACCATAAAGCGGCACAAGTGGTCAGTCCTGAAGAGTCGGAAGCTGGCA
TATCGGCCGCCCAAGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 986
Start on reference sequence 9
Mutations:
None
Discrepancy:
g265c,V89L
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CYC1
Symbol Nomenclature : CYC1
SYNONYM : MC3DN6; UQCR4
Designation : complex III subunit 4|complex III subunit IV|cytochrome b-c1 complex subunit 4|cytochrome c1, heme protein, mitochondrial|ubiquinol-cytochrome-c reductase complex cytochrome c1 subunit
Full Nomenclature : cytochrome c-1
GENEID : 1537
GI : 61366536
GenBank Accession : AY890936
HGNC : 2579
MIM : 123980
Vega : OTTHUMG00000165242
Target GenBank: NM_001916

 

Reference Sequence Alignment

  

HsCD00000654      ATGGCGGCAGCTGCGGCTTCGCTTCGCGGGGTAGTGTTGGGCCCGCGGGGCGCGGGGCTC
NM_001916.4       ATGGCGGCAGCTGCGGCTTCGCTTCGCGGGGTAGTGTTGGGCCCGCGGGGCGCGGGGCTC
                  ************************************************************

HsCD00000654      CCGGGCGCGCGTGCCCGGGGTCTGCTGTGCAGCGCGCGGCCCGGGCAGCTCCCGCTACGG
NM_001916.4       CCGGGCGCGCGTGCCCGGGGTCTGCTGTGCAGCGCGCGTCCCGGGCAGCTCCCGCTACGG
                  ************************************** *********************

HsCD00000654      ACACCTCAGGCAGTGGCCTTGTCGTCGAAGTCTGGCCTTTCCCGAGGCCGGAAAGTGATG
NM_001916.4       ACACCTCAGGCAGTGGCCTTGTCGTCGAAGTCTGGCCTTTCCCGAGGCCGGAAAGTGATG
                  ************************************************************

HsCD00000654      CTGTCAGCGCTGGGCATGCTGGCGGCAGGGGGTGCGGGGCTGGCCGTGGCTCTGCATTCG
NM_001916.4       CTGTCAGCGCTGGGCATGCTGGCGGCAGGGGGTGCGGGGCTGGCCGTGGCTCTGCATTCG
                  ************************************************************

HsCD00000654      GCTGTGAGTGCCAGTGACCTGGAGCTGCACCCCCCCAGCTATCCGTGGTCTCACCGTGGC
NM_001916.4       GCTGTGAGTGCCAGTGACCTGGAGCTGCACCCCCCCAGCTATCCGTGGTCTCACCGTGGC
                  ************************************************************

HsCD00000654      CTCCTCTCTTCCTTGGACCACACCAGCATCCGGAGGGGTTTCCAGGTATATAAGCAGGTG
NM_001916.4       CTCCTCTCTTCCTTGGACCACACCAGCATCCGGAGGGGTTTCCAGGTATATAAGCAGGTG
                  ************************************************************

HsCD00000654      TGCGCCTCCTGCCACAGCATGGACTTCGTGGCCTACCGCCACCTGGTGGGCGTGTGCTAC
NM_001916.4       TGCGCCTCCTGCCACAGCATGGACTTCGTGGCCTACCGCCACCTGGTGGGCGTGTGCTAC
                  ************************************************************

HsCD00000654      ACGGAGGATGAAGCTAAGGAGCTGGCTGCGGAGGTGGAGGTTCAAGACGGCCCCAATGAA
NM_001916.4       ACGGAGGATGAAGCTAAGGAGCTGGCTGCGGAGGTGGAGGTTCAAGACGGCCCCAATGAA
                  ************************************************************

HsCD00000654      GATGGGGAGATGTTCATGCGGCCAGGGAAGCTGTTCGACTATTTCCCAAAACCATACCCC
NM_001916.4       GATGGGGAGATGTTCATGCGGCCAGGGAAGCTGTTCGACTATTTCCCAAAACCATACCCC
                  ************************************************************

HsCD00000654      AACAGTGAGGCTGCTCGAGCTGCCAACAACGGAGCATTGCCCCCTGACCTCAGCTACATC
NM_001916.4       AACAGTGAGGCTGCTCGAGCTGCCAACAACGGAGCATTGCCCCCTGACCTCAGCTACATC
                  ************************************************************

HsCD00000654      GTGCGAGCTAGGCATGGTGGTGAGGACTACGTCTTCTCCCTGCTCACGGGCTACTGCGAG
NM_001916.4       GTGCGAGCTAGGCATGGTGGTGAGGACTACGTCTTCTCCCTGCTCACGGGCTACTGCGAG
                  ************************************************************

HsCD00000654      CCACCCACCGGGGTGTCACTGCGGGAAGGTCTCTACTTCAACCCCTACTTTCCTGGCCAG
NM_001916.4       CCACCCACCGGGGTGTCACTGCGGGAAGGTCTCTACTTCAACCCCTACTTTCCTGGCCAG
                  ************************************************************

HsCD00000654      GCCATTGCCATGGCCCCTCCCATCTACACAGATGTCTTAGAGTTTGACGATGGCACCCCA
NM_001916.4       GCCATTGCCATGGCCCCTCCCATCTACACAGATGTCTTAGAGTTTGACGATGGCACCCCA
                  ************************************************************

HsCD00000654      GCTACCATGTCCCAGATAGCCAAGGATGTGTGCACCTTCCTGCGCTGGGCATCTGAGCCA
NM_001916.4       GCTACCATGTCCCAGATAGCCAAGGATGTGTGCACCTTCCTGCGCTGGGCATCTGAGCCA
                  ************************************************************

HsCD00000654      GAGCACGACCATCGAAAACGCATGGGGCTCAAGATGTTGATGATGATGGCTCTGCTGGTG
NM_001916.4       GAGCACGACCATCGAAAACGCATGGGGCTCAAGATGTTGATGATGATGGCTCTGCTGGTG
                  ************************************************************

HsCD00000654      CCCCTGGTCTACACCATAAAGCGGCACAAGTGGTCAGTCCTGAAGAGTCGGAAGCTGGCA
NM_001916.4       CCCCTGGTCTACACCATAAAGCGGCACAAGTGGTCAGTCCTGAAGAGTCGGAAGCTGGCA
                  ************************************************************

HsCD00000654      TATCGGCCGCCCAAGTTG
NM_001916.4       TATCGGCCGCCCAAGTGA
                  **************** .

Pathways

Panther : FAS signaling pathway
Panther : Huntington disease
Reactome : Metabolism
Reactome : Metabolism of proteins
Reactome : Mitochondrial protein import
Reactome : Respiratory electron transport
Reactome : Respiratory electron transport, ATP synthesis by chemiosmotic coupling, and heat production by uncoupling proteins.
Reactome : The citric acid (TCA) cycle and respiratory electron transport

Protein

UniProt : P08574
HPRD : 00480

Clone

Cloning Information : 8192
HIP Master Clone ID : 7750
Original Clone ID : FLH008192.01L

Organism


TAX_ID : 9606
Species Specific ID: 1537

Genome

Chromosome : 8
Map Location : 8q24.3
Ensembl : ENSG00000179091

Reagents

Labome : cytochrome-c-antibody