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MXD4 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  MXD4
Gene Name:  MAX dimerization protein 4
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH008444.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000661

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 630nts         Open reading frame : 1 to 630
Reference Sequence Alignment

ATGGAGCTGAACTCCCTGCTGATCCTGCTGGAGGCGGCCGAGTACCTGGAGCGCAGGGAT
CGTGAGGCCGAGCACGGCTACGCCTCGGTGCTGCCCTTCGACGGCGACTTCGCCAGGGAG
AAAACAAAGGCGGCCGGCCTGGTGCGCAAGGCCCCGAACAACAGGTCTTCACACAACGAG
CTAGAAAAGCACAGACGAGCCAAACTCAGGCTGTACCTTGAGCAGCTCAAGCAACTGGTG
CCCCTGGGCCCCGACAGCACCCGCCACACCACGCTGAGCCTCCTGAAGCGGGCCAAGGTG
CACATCAAGAAACTGGAGGAGCAGGACCGCCGGGCACTGAGCATCAAGGAGCAGCTGCAG
CAGGAGCATCGTTTCCTGAAGCGGCGCCTGGAGCAGCTGTCGGTGCAGAGCGTGGAGCGC
GTGCGCACAGATAGCACGGGCTCTGCTGTCTCCACGGACGACTCAGAGCAAGAAGTGGAC
ATAGAGGGCATGGAGTTTGGCCCTGGTGAGCTGGACAGTGTTGGCAGCAGCAGTGACGCG
GACGACCACTACAGCCTGCAGAGTGGCACCGGCGGCGACAGTGGCTTCGGGCCCCACTGC
CGGCGGCTGGGCCGCCCCGCCCTCTCGTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 662
Start on reference sequence 33
Mutations:
None
Discrepancy:
a63t
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : MXD4
Symbol Nomenclature : MXD4
SYNONYM : MAD4; MST149; MSTP149; bHLHc12
Designation : Mad4 homolog|class C basic helix-loop-helix protein 12|max dimerization protein 4|max dimerizer 4|max-associated protein 4|max-interacting transcriptional repressor MAD4
Full Nomenclature : MAX dimerization protein 4
GENEID : 10608
Locus Tag : RP11-478C1.1
GI : 61356774
GenBank Accession : AY888339
HGNC : 13906
Vega : OTTHUMG00000090243
Target GenBank: NM_006454

 

Reference Sequence Alignment

  

HsCD00000661      ATGGAGCTGAACTCCCTGCTGATCCTGCTGGAGGCGGCCGAGTACCTGGAGCGCAGGGAT
NM_006454.2       ATGGAGCTGAACTCCCTGCTGATCCTGCTGGAGGCGGCCGAGTACCTGGAGCGCAGGGAT
                  ************************************************************

HsCD00000661      CGTGAGGCCGAGCACGGCTACGCCTCGGTGCTGCCCTTCGACGGCGACTTCGCCAGGGAG
NM_006454.2       CGAGAGGCCGAGCACGGCTACGCCTCGGTGCTGCCCTTCGACGGCGACTTCGCCAGGGAG
                  **:*********************************************************

HsCD00000661      AAAACAAAGGCGGCCGGCCTGGTGCGCAAGGCCCCGAACAACAGGTCTTCACACAACGAG
NM_006454.2       AAAACAAAGGCGGCCGGCCTGGTGCGCAAGGCCCCGAACAACAGGTCTTCACACAACGAG
                  ************************************************************

HsCD00000661      CTAGAAAAGCACAGACGAGCCAAACTCAGGCTGTACCTTGAGCAGCTCAAGCAACTGGTG
NM_006454.2       CTAGAAAAGCACAGACGAGCCAAACTCAGGCTGTACCTTGAGCAGCTCAAGCAACTGGTG
                  ************************************************************

HsCD00000661      CCCCTGGGCCCCGACAGCACCCGCCACACCACGCTGAGCCTCCTGAAGCGGGCCAAGGTG
NM_006454.2       CCCCTGGGCCCCGACAGCACCCGCCACACCACGCTGAGCCTCCTGAAGCGGGCCAAGGTG
                  ************************************************************

HsCD00000661      CACATCAAGAAACTGGAGGAGCAGGACCGCCGGGCACTGAGCATCAAGGAGCAGCTGCAG
NM_006454.2       CACATCAAGAAACTGGAGGAGCAGGACCGCCGGGCACTGAGCATCAAGGAGCAGCTGCAG
                  ************************************************************

HsCD00000661      CAGGAGCATCGTTTCCTGAAGCGGCGCCTGGAGCAGCTGTCGGTGCAGAGCGTGGAGCGC
NM_006454.2       CAGGAGCATCGTTTCCTGAAGCGGCGCCTGGAGCAGCTGTCGGTGCAGAGCGTGGAGCGC
                  ************************************************************

HsCD00000661      GTGCGCACAGATAGCACGGGCTCTGCTGTCTCCACGGACGACTCAGAGCAAGAAGTGGAC
NM_006454.2       GTGCGCACAGATAGCACGGGCTCTGCTGTCTCCACGGACGACTCAGAGCAAGAAGTGGAC
                  ************************************************************

HsCD00000661      ATAGAGGGCATGGAGTTTGGCCCTGGTGAGCTGGACAGTGTTGGCAGCAGCAGTGACGCG
NM_006454.2       ATAGAGGGCATGGAGTTTGGCCCTGGTGAGCTGGACAGTGTTGGCAGCAGCAGTGACGCG
                  ************************************************************

HsCD00000661      GACGACCACTACAGCCTGCAGAGTGGCACCGGCGGCGACAGTGGCTTCGGGCCCCACTGC
NM_006454.2       GACGACCACTACAGCCTGCAGAGTGGCACCGGCGGCGACAGTGGCTTCGGGCCCCACTGC
                  ************************************************************

HsCD00000661      CGGCGGCTGGGCCGCCCCGCCCTCTCGTAG
NM_006454.2       CGGCGGCTGGGCCGCCCCGCCCTCTCGTAG
                  ******************************

Pathways

NCI : Validated targets of C-MYC transcriptional repression

Protein

SMART domain : HLH : helix loop helix domain
UniProt : Q14582
HPRD : 10103

Clone

Cloning Information : 8444
HIP Master Clone ID : 10939
Original Clone ID : FLH008444.01X

Organism


TAX_ID : 9606
Species Specific ID: 10608

Genome

Chromosome : 4
Map Location : 4p16.3
Ensembl : ENSG00000123933

Reagents

Labome : MXD4-antibody