Detailed Vector Information: pET15_8HMbpTEV_NESG

Explanation of Terms

Gene:
Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID:
None
Type:
No insert
Vector Name:
pET15_8HMbpTEV_NESG
Source:
Northeast Structural Genomics Consortium
Description:
Bacterial expression vector that increases protein solubility with T7 promoter with TEV-cleavable, 3C-cleavable N-temrinal 8xHis MBP tag; ampicillin resistance in bacteria; restriction enzyme cloning
Comments:
None
Publications:
None
Authors:
Rutgers University
Gaetano T. Montleione
Thomas B. Acton
Huang Wang
Northeast Structural Genomics Consortium
PSI:Biology
EvNO00665443
Price: 
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AVAILABLE
Academic and non-profit labs
Special MTA: PSI/NESG

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition: 100 ug/mL ampicillin
Growth Condition: Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments: Commonly used conditions for ampicillin resistant plasmid clones.