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Detailed Vector Information: pANT7_GST-control

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Explanation of Terms


Gene: Gene Symbol:  No insert
Gene Name:  No insert
Original Clone ID: None
Type: No insert
Vector Name: pANT7_GST-control              
Source: Center for Personalized Diagnostics
Description: In vitro expression vector expressing two GSTs as a control vector for clones in pANT7_cGST; ampicillin resistance in bacteria; recombinational cloning.
Comments: None
Publications: None
Authors: Center for Personalized Diagnostics
Arizona State University
Shane Miersch
EvNO00665463

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AVAILABLE
No restriction
Special MTA: None

 

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
       DyNA Vector Map

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Vector Name: pANT7_GST-control

pANT7_GST-control in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  Unknown
Reverse:  Unknown
Description: In vitro expression vector expressing two GSTs as a control vector for clones in pANT7_cGST; ampicillin resistance in bacteria; recombinational cloning.
Comments: This vector is a control vector for clones in pANT7_cGST that only expresses GST.
Size (bp): 4982
Parent Vector: Name: pANT7_cGST
Description:
Properties: Gateway, in vitro transcription, recombinational cloning, with tag/fusion/marker
Author Name: Center for Personalized Diagnostics
Arizona State University
Shane Miersch
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE ColE1 bacterial origin 2267 2949
bacterial origin f1 f1 single stranded origin 4475 4781
enhancer CITE CITE enhancer 17 515
gene fragment GST-2 GST expressed in tandem with GST from the pANT7_cGST vector 576 1241
promoter T7 T7 promoter 4966 4982
recombination site AttB2 attB2 recombinational cloning site 1246 1270
recombination site AttB1 attB1 recombinational cloning site 549 573
selectable marker AmpR ampicillin resistance 3047 3706
tag GST GST 1300 1965
trxn termination sequence T7 term T7 terminator 2038 2085

 

Species Specific ID: None
Target GenBank: None