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ZFP36L1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  ZFP36L1
Gene Name:  ZFP36 ring finger protein-like 1
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH008367.01L
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / Yes
Publications: None
Authors: HIP
HsCD00000668

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1017nts         Open reading frame : 1 to 1017
Reference Sequence Alignment

ATGACCACCACCCTCGTGTCTGCCACCATCTTCGACTTGAGCGAAGTTTTATGCAAGGGT
AACAAGATGCTCAACTATAGTGCTCCCAGTGCAGGGGTTTGCCTGCTGGACAGAAAGGCA
GTGGGCACCCCTGCTGGTGGGGGCTTCCCTCGGAGGCACTCAGTCACCCTGCCCAGCTCC
AAGTTCCACCAGAACCAGCTCCTCAGCAGCCTCAAGGGTGAGCCAGCCCCCGCTCTGAGC
TCGCGAGACAGCCGCTTCCGAGACCGCTCCTTCTCGGAAGGGGGCGAGCGGCTGCTGCCC
ACCCAGAAGCAGCCCGGGGGCGGCCAGGTCAACTCCAGCCGCTACAAGACGGAGCTGTGC
CGCCCCTTTGAGGAAAACGGTGCCTGTAAGTACGGGGACAAGTGCCAGTTCGCACACGGC
ATCCACGAGCTCCGCAGCCTGACCCGCCACCCCAAGTACAAGACGGAGCTGTGCCGCACC
TTCCACACCATCGGCTTTTGCCCCTACGGGCCCCGCTGCCACTTCATCCACAACGCTGAA
GAGCGCCGTGCCCTGGCCGGGGCCCGGGACCTCTCCGCTGACCGTCCCCGCCTCCAGCAT
AGCTTTAGCTTTGCTGGGTTTCCCAGTGCCGCTGCCACCGCCGCTGCCACCGGGCTGCTG
GACAGCCCCACGTCCATCACCCCACCCCCTATTCTGAGCGCCGATGACCTCCTGGGCTCA
CCTACCCTGCCCGATGGCACCAATAACCCTTTTGCCTTCTCCAGCCAGGAGCTGGCAAGC
CTCTTTGCCCCTAGCATGGGGCTGCCCGGGGGTGGCTCCCCGACCACCTTCCTCTTCCGG
CCCATGTCCGAGTCCCCTCACATGTTTGACTCTCCCCCCAGCCCTCAGGATTCTCTCTCG
GACCAGGAGGGCTACCTGAGCAGCTCCAGCAGCAGCCACAGTGGCTCAGACTCCCCGACC
TTGGACAACTCAAGACGCCTGCCCATCTTCAGCAGACTTTCCATCTCAGATGACTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1147
Start on reference sequence 131
Mutations:
None
Discrepancy:
g98t,G33V
                                

5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : ZFP36L1
Symbol Nomenclature : ZFP36L1
SYNONYM : BRF1; Berg36; ERF-1; ERF1; RNF162B; TIS11B; cMG1
Designation : EGF-response factor 1|butyrate response factor 1|early response factor Berg36|zinc finger protein 36, C3H type-like 1|zinc finger protein 36, C3H1 type-like 1|zinc finger protein, C3H type, 36-like 1
Full Nomenclature : ZFP36 ring finger protein-like 1
GENEID : 677
GI : 61366591
GenBank Accession : AY890944
HGNC : 1107
MIM : 601064
Vega : OTTHUMG00000171385
Target GenBank: NM_004926

 

Reference Sequence Alignment

  

HsCD00000668        ------------------------------------------------------------
NM_001244701.1      ATGGGGAGGGCCCGGAAGTCGGGCCTGGGACAGAGGAGCCGGCCCACCGCCTCCCGGTCG
NM_001244698.1      ------------------------------------------------------------
NM_004926.3         ------------------------------------------------------------
                                                                                

HsCD00000668        ------------------------------------------------------------
NM_001244701.1      GAAGCGGCAGTGCAGCCCGGAGTCAGAAAGGCGAGGGGCGCCGGGAACTGGCGTGTGGGA
NM_001244698.1      ------------------------------------------------------------
NM_004926.3         ------------------------------------------------------------
                                                                                

HsCD00000668        ------------------------------------------------------------
NM_001244701.1      CTCCAGACAGGAGAGGCTGCGCCTTCCCCGCACCGGGACCTTCGCGACACACCAGATCCT
NM_001244698.1      ------------------------------------------------------------
NM_004926.3         ------------------------------------------------------------
                                                                                

HsCD00000668        ---------------------------ATGACCACCACCCTCGTGTCTGCCACCATCTTC
NM_001244701.1      CGCCCCTGGCTCGCGCGAACGCACAGGATGACCACCACCCTCGTGTCTGCCACCATCTTC
NM_001244698.1      ---------------------------ATGACCACCACCCTCGTGTCTGCCACCATCTTC
NM_004926.3         ---------------------------ATGACCACCACCCTCGTGTCTGCCACCATCTTC
                                               *********************************

HsCD00000668        GACTTGAGCGAAGTTTTATGCAAGGGTAACAAGATGCTCAACTATAGTGCTCCCAGTGCA
NM_001244701.1      GACTTGAGCGAAGTTTTATGCAAGGGTAACAAGATGCTCAACTATAGTGCTCCCAGTGCA
NM_001244698.1      GACTTGAGCGAAGTTTTATGCAAGGGTAACAAGATGCTCAACTATAGTGCTCCCAGTGCA
NM_004926.3         GACTTGAGCGAAGTTTTATGCAAGGGTAACAAGATGCTCAACTATAGTGCTCCCAGTGCA
                    ************************************************************

HsCD00000668        GGGGTTTGCCTGCTGGACAGAAAGGCAGTGGGCACCCCTGCTGGTGGGGGCTTCCCTCGG
NM_001244701.1      GGGGGTTGCCTGCTGGACAGAAAGGCAGTGGGCACCCCTGCTGGTGGGGGCTTCCCTCGG
NM_001244698.1      GGGGGTTGCCTGCTGGACAGAAAGGCAGTGGGCACCCCTGCTGGTGGGGGCTTCCCTCGG
NM_004926.3         GGGGGTTGCCTGCTGGACAGAAAGGCAGTGGGCACCCCTGCTGGTGGGGGCTTCCCTCGG
                    **** *******************************************************

HsCD00000668        AGGCACTCAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGCCTC
NM_001244701.1      AGGCACTCAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGCCTC
NM_001244698.1      AGGCACTCAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGCCTC
NM_004926.3         AGGCACTCAGTCACCCTGCCCAGCTCCAAGTTCCACCAGAACCAGCTCCTCAGCAGCCTC
                    ************************************************************

HsCD00000668        AAGGGTGAGCCAGCCCCCGCTCTGAGCTCGCGAGACAGCCGCTTCCGAGACCGCTCCTTC
NM_001244701.1      AAGGGTGAGCCAGCCCCCGCTCTGAGCTCGCGAGACAGCCGCTTCCGAGACCGCTCCTTC
NM_001244698.1      AAGGGTGAGCCAGCCCCCGCTCTGAGCTCGCGAGACAGCCGCTTCCGAGACCGCTCCTTC
NM_004926.3         AAGGGTGAGCCAGCCCCCGCTCTGAGCTCGCGAGACAGCCGCTTCCGAGACCGCTCCTTC
                    ************************************************************

HsCD00000668        TCGGAAGGGGGCGAGCGGCTGCTGCCCACCCAGAAGCAGCCCGGGGGCGGCCAGGTCAAC
NM_001244701.1      TCGGAAGGGGGCGAGCGGCTGCTGCCCACCCAGAAGCAGCCCGGGGGCGGCCAGGTCAAC
NM_001244698.1      TCGGAAGGGGGCGAGCGGCTGCTGCCCACCCAGAAGCAGCCCGGGGGCGGCCAGGTCAAC
NM_004926.3         TCGGAAGGGGGCGAGCGGCTGCTGCCCACCCAGAAGCAGCCCGGGGGCGGCCAGGTCAAC
                    ************************************************************

HsCD00000668        TCCAGCCGCTACAAGACGGAGCTGTGCCGCCCCTTTGAGGAAAACGGTGCCTGTAAGTAC
NM_001244701.1      TCCAGCCGCTACAAGACGGAGCTGTGCCGCCCCTTTGAGGAAAACGGTGCCTGTAAGTAC
NM_001244698.1      TCCAGCCGCTACAAGACGGAGCTGTGCCGCCCCTTTGAGGAAAACGGTGCCTGTAAGTAC
NM_004926.3         TCCAGCCGCTACAAGACGGAGCTGTGCCGCCCCTTTGAGGAAAACGGTGCCTGTAAGTAC
                    ************************************************************

HsCD00000668        GGGGACAAGTGCCAGTTCGCACACGGCATCCACGAGCTCCGCAGCCTGACCCGCCACCCC
NM_001244701.1      GGGGACAAGTGCCAGTTCGCACACGGCATCCACGAGCTCCGCAGCCTGACCCGCCACCCC
NM_001244698.1      GGGGACAAGTGCCAGTTCGCACACGGCATCCACGAGCTCCGCAGCCTGACCCGCCACCCC
NM_004926.3         GGGGACAAGTGCCAGTTCGCACACGGCATCCACGAGCTCCGCAGCCTGACCCGCCACCCC
                    ************************************************************

HsCD00000668        AAGTACAAGACGGAGCTGTGCCGCACCTTCCACACCATCGGCTTTTGCCCCTACGGGCCC
NM_001244701.1      AAGTACAAGACGGAGCTGTGCCGCACCTTCCACACCATCGGCTTTTGCCCCTACGGGCCC
NM_001244698.1      AAGTACAAGACGGAGCTGTGCCGCACCTTCCACACCATCGGCTTTTGCCCCTACGGGCCC
NM_004926.3         AAGTACAAGACGGAGCTGTGCCGCACCTTCCACACCATCGGCTTTTGCCCCTACGGGCCC
                    ************************************************************

HsCD00000668        CGCTGCCACTTCATCCACAACGCTGAAGAGCGCCGTGCCCTGGCCGGGGCCCGGGACCTC
NM_001244701.1      CGCTGCCACTTCATCCACAACGCTGAAGAGCGCCGTGCCCTGGCCGGGGCCCGGGACCTC
NM_001244698.1      CGCTGCCACTTCATCCACAACGCTGAAGAGCGCCGTGCCCTGGCCGGGGCCCGGGACCTC
NM_004926.3         CGCTGCCACTTCATCCACAACGCTGAAGAGCGCCGTGCCCTGGCCGGGGCCCGGGACCTC
                    ************************************************************

HsCD00000668        TCCGCTGACCGTCCCCGCCTCCAGCATAGCTTTAGCTTTGCTGGGTTTCCCAGTGCCGCT
NM_001244701.1      TCCGCTGACCGTCCCCGCCTCCAGCATAGCTTTAGCTTTGCTGGGTTTCCCAGTGCCGCT
NM_001244698.1      TCCGCTGACCGTCCCCGCCTCCAGCATAGCTTTAGCTTTGCTGGGTTTCCCAGTGCCGCT
NM_004926.3         TCCGCTGACCGTCCCCGCCTCCAGCATAGCTTTAGCTTTGCTGGGTTTCCCAGTGCCGCT
                    ************************************************************

HsCD00000668        GCCACCGCCGCTGCCACCGGGCTGCTGGACAGCCCCACGTCCATCACCCCACCCCCTATT
NM_001244701.1      GCCACCGCCGCTGCCACCGGGCTGCTGGACAGCCCCACGTCCATCACCCCACCCCCTATT
NM_001244698.1      GCCACCGCCGCTGCCACCGGGCTGCTGGACAGCCCCACGTCCATCACCCCACCCCCTATT
NM_004926.3         GCCACCGCCGCTGCCACCGGGCTGCTGGACAGCCCCACGTCCATCACCCCACCCCCTATT
                    ************************************************************

HsCD00000668        CTGAGCGCCGATGACCTCCTGGGCTCACCTACCCTGCCCGATGGCACCAATAACCCTTTT
NM_001244701.1      CTGAGCGCCGATGACCTCCTGGGCTCACCTACCCTGCCCGATGGCACCAATAACCCTTTT
NM_001244698.1      CTGAGCGCCGATGACCTCCTGGGCTCACCTACCCTGCCCGATGGCACCAATAACCCTTTT
NM_004926.3         CTGAGCGCCGATGACCTCCTGGGCTCACCTACCCTGCCCGATGGCACCAATAACCCTTTT
                    ************************************************************

HsCD00000668        GCCTTCTCCAGCCAGGAGCTGGCAAGCCTCTTTGCCCCTAGCATGGGGCTGCCCGGGGGT
NM_001244701.1      GCCTTCTCCAGCCAGGAGCTGGCAAGCCTCTTTGCCCCTAGCATGGGGCTGCCCGGGGGT
NM_001244698.1      GCCTTCTCCAGCCAGGAGCTGGCAAGCCTCTTTGCCCCTAGCATGGGGCTGCCCGGGGGT
NM_004926.3         GCCTTCTCCAGCCAGGAGCTGGCAAGCCTCTTTGCCCCTAGCATGGGGCTGCCCGGGGGT
                    ************************************************************

HsCD00000668        GGCTCCCCGACCACCTTCCTCTTCCGGCCCATGTCCGAGTCCCCTCACATGTTTGACTCT
NM_001244701.1      GGCTCCCCGACCACCTTCCTCTTCCGGCCCATGTCCGAGTCCCCTCACATGTTTGACTCT
NM_001244698.1      GGCTCCCCGACCACCTTCCTCTTCCGGCCCATGTCCGAGTCCCCTCACATGTTTGACTCT
NM_004926.3         GGCTCCCCGACCACCTTCCTCTTCCGGCCCATGTCCGAGTCCCCTCACATGTTTGACTCT
                    ************************************************************

HsCD00000668        CCCCCCAGCCCTCAGGATTCTCTCTCGGACCAGGAGGGCTACCTGAGCAGCTCCAGCAGC
NM_001244701.1      CCCCCCAGCCCTCAGGATTCTCTCTCGGACCAGGAGGGCTACCTGAGCAGCTCCAGCAGC
NM_001244698.1      CCCCCCAGCCCTCAGGATTCTCTCTCGGACCAGGAGGGCTACCTGAGCAGCTCCAGCAGC
NM_004926.3         CCCCCCAGCCCTCAGGATTCTCTCTCGGACCAGGAGGGCTACCTGAGCAGCTCCAGCAGC
                    ************************************************************

HsCD00000668        AGCCACAGTGGCTCAGACTCCCCGACCTTGGACAACTCAAGACGCCTGCCCATCTTCAGC
NM_001244701.1      AGCCACAGTGGCTCAGACTCCCCGACCTTGGACAACTCAAGACGCCTGCCCATCTTCAGC
NM_001244698.1      AGCCACAGTGGCTCAGACTCCCCGACCTTGGACAACTCAAGACGCCTGCCCATCTTCAGC
NM_004926.3         AGCCACAGTGGCTCAGACTCCCCGACCTTGGACAACTCAAGACGCCTGCCCATCTTCAGC
                    ************************************************************

HsCD00000668        AGACTTTCCATCTCAGATGACTTG
NM_001244701.1      AGACTTTCCATCTCAGATGACTAA
NM_001244698.1      AGACTTTCCATCTCAGATGACTAA
NM_004926.3         AGACTTTCCATCTCAGATGACTAA
                    **********************:.

Pathways

NCI : Validated targets of C-MYC transcriptional repression
Reactome : Butyrate Response Factor 1 (BRF1) destabilizes mRNA
Reactome : Gene Expression
Reactome : Metabolism of RNA
Reactome : Metabolism of mRNA
Reactome : Regulation of mRNA stability by proteins that bind AU-rich elements

Protein

SMART domain : ZnF_C3H1 : zinc finger
UniProt : Q07352
HPRD : 03041

Clone

Cloning Information : 8367
HIP Master Clone ID : 10862
Original Clone ID : FLH008367.01L

Organism


TAX_ID : 9606
Species Specific ID: 677

Genome

Chromosome : 14
Map Location : 14q22-q24
Ensembl : ENSG00000185650

Reagents

Labome : ZFP36L1-antibody