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asd-2 (Bacillus anthracis str. Ames) in pDONR221 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  asd-2
Gene Name:  aspartate-semialdehyde dehydrogenase
Sequence              Map: pDONR cofig.pdf
Original Clone ID: FLH247609.01L
Keyword: None
Species: Bacillus anthracis str. Ames
Type: cDNA
Vector Name: pDONR221               Format:  FUSION
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: None
Authors: HIP
BaCD00067114

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 1047nts         Open reading frame : 1 to 1047

ATGGAAAAGCAAAAAACTTTTCATGTCGCTGTAGTTGGAGCAACCGGCGCAGTTGGTGAA
CAAATGTTAAATACTTTAGAGAAACGAGAATTTCCAATCGGGAAGTTAACGTTACTTTCA
TCTAAACGATCTGCAGGTAAGAAACTTGTATTTAAAGGCGAAGAATTTACAGTTCAAGAG
GCAACTCCTGAAAGTTTTGAAGGAGTAGATATCGCACTATTTAGTGCAGGTGGATCTGTA
TCAAAACAATTAGCGCCAGAAGCAGCAAAGCGCGGTGCGATTGTTGTTGATAATACAAGT
GCATTCCGTATGACAGAAAACGTGCCACTTGTTGTACCTGAAGTAAATGAAAACGACTTA
AAAGAACATAATGGTATTATTGCAAATCCAAACTGTTCTACAATTCAAATGGTAGTAGCT
CTTGAGCCAGTTCGTCAGCAATATGGTTTAAAACGAGTAATCGTTTCCACATATCAAGCT
GTATCAGGTGCTGGTGCGGCAGCGATTGAAGAACTTCATGAACAATCACAAGCAATCTTA
AATGGCGAAGAAGTGAAGGCGAATGTTTTACCTGTATCAGGTGATAAGAAACATTTTCCA
ATTGCTTTCAACGCGATTCCACAGATTGATAAGTTCCAAGATAATGGATTTACGTTTGAA
GAAATGAAAATGATTAATGAAACGAAAAAAATTATGCATATGCCTGAATTAGAAGTAGCG
GCAACATGTGTACGTTTACCAGTTGTATCAGGTCACTCTGAGTCTGTTTACATTGAAGTA
GAAAAAGAAGGCGTAACAGTAGAAGAATTAAAGAGCTTACTTGCAAATGCGGAAGGTATC
GTTCTACAAGATAATCCAGAAGAGCAGCTATATCCAATGCCAGCTACTGCAGTAGGTAAA
AACGAAGTATTCGTGGGAAGAATTCGTAAAGATTTAAATAACGATAAAGGATTCCATCTT
TGGGTCGTATCTGATAACTTATTAAAAGGCGCAGCATGGAATTCTGTTCAAATTGCAGAG
CGCTTAGTAAAATTACAATTAGTGTTG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 1047
Start on reference sequence 1
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5p GTACAAAAAAGCAGGCTCCACC(atg-goi)

3' Linker Sequence:
5p (goi-stop)GACCCAGCTTTCTTGTAC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  kanamycin
Bacterial Selection Condition:  50ug/mL kanamycin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Conditions for Gateway-type vectors in recombined (with insert) form and other kanamycin resistant vectors.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR221

pDONR221 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  U19
Reverse:  AttP2
Description: Recombinational donor/master vector with M13 F and R primer sites; kanamycin resistance; recombinational cloning.
Comments: The position of features were determined for the unrecombined (empty) form of the vector, which is described in detail on the Invitrogen website.
Size (bp): 4762
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4086 4759
negative selection marker ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1502 1197
primer site M13 forward primer M13 Forward priming site 537 552
primer site M13 reverse primer M13 Reverse priming site 3027 3034
recombination site attP1 attP recombination site 1 LOST in recombined (with insert) form 570 801
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only, position is approximate 570 801
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only, position is approximate 2985 2754
recombination site attP2 attP recombination site 2 LOST in recombined (with insert) form 2985 2754
selectable marker CmR chloramphenicol resistance gene, LOST in recombined (with insert) form 2506 1847
selectable marker KanR kanamycin resistance gene 3156 3965
trxn termination sequence rrnB T2 rrnB T2 transcription termination sequence 295 268
trxn termination sequence rrnB T1 rrnB T1 transcription termination sequence 470 427

 

  • Gene
  • Protein
  • Clone
  • Organism
  • Reagents

Gene

Target GenBank: NP_846181

 

Clone

Cloning Information : 247609
HIP Master Clone ID : 189273
Original Clone ID : FLH247609.01L

Organism


Species Specific ID: BA3937

Reagents

Labome : asd-2-cDNA