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KAI1 (Homo sapiens) in pDNR-Dual (Creator donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  CD82
Gene Name:  CD82 molecule
Sequence              Map: pDNR-Dual_with_human_insert.pdf
Original Clone ID: FLH020067.01X
Keyword: None
Species: Homo sapiens
Type: cDNA
Vector Name: pDNR-Dual               Format:  CLOSED
Source: HIP
Description: None
Comments: None
Mutation/
Discrepancy :
No / No
Publications: PMID: 16512675
Title: Functional proteomics approach to investigate the biological activities of cDNAs implicated in breast cancer.
Authors: HIP
HsCD00000701

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 804nts         Open reading frame : 1 to 804
Reference Sequence Alignment

ATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAACTTGATCTTC
TTTATCCTGGGCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGACAAGAGCAGT
TTCATCTCTGTCCTGCAAACCTCCTCCAGCTCGCTTAGGATGGGGGCCTATGTCTTCATC
GGCGTGGGGGCAGTCACTATGCTCATGGGCTTCCTGGGCTGCATCGGCGCCGTCAACGAG
GTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCCTGATCCTCATTGCCCAGGTG
ACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGATGGGCGGCATC
GTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCAGGATGCCTGG
GACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAACTGGACAGAC
AACGCTGAGCTCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGAAGTCAAGGGG
GAAGAGGACAACAGCCTTTCTGTGAGGAAGGGCTTCTGCGAGGCCCCCGGCAACAGGACC
CAGAGTGGCAACCACCCTGAGGACTGGCCTGTGTACCAGGAGGGCTGCATGGAGAAGGTG
CAGGCGTGGCTGCAGGAGAACCTGGGCATCATCCTCGGCGTGGGCGTGGGTGTGGCCATC
ATCGAGCTCCTGGGGATGGTCCTGTCCATCTGCTTGTGCCGGCACGTCCATTCCGAAGAC
TACAGCAAGGTCCCCAAGTACTAG


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: Sequence Verification
Reference Sequence Annotations:
End on reference sequence 985
Start on reference sequence 182
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
5P GCGGCCGCATAACTTCGTATAGCATACATTATACGAAGTTATCAGTC
GACACC(ATG-GOI)

3' Linker Sequence:
5p (goi-stop)GGAAGCTTTCTAGACCATTCGTTTGGCGCGCGGGCCC

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  ampicillin
Bacterial Selection Condition:  100 ug/mL ampicillin
Growth Condition:  Growth with the single antibiotic in LB at 37 degrees is recommended.
Comments:  Commonly used conditions for ampicillin resistant plasmid clones.
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDNR-Dual

pDNR-Dual in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  Unknown
Description: Recombinational donor/master vector with 6xHN tag ORF, T7 and M13 primer sites; ampicillin resistance; restriction enzyme cloning (into) and recombinational cloning (from).
Comments: The position of features were detemined for the empty form of the vector, which is described in detail on the BD/Clontech website. Subcloning into the MCS was performed using the infusion reaction strategy and some restriction sites in the MCS may be lost after an insert is added.
Size (bp): 4938
Parent Vector: None
Empty Vector: None
Properties: Creator, donor (entry), loxP, multiple cloning site, recombinational cloning, with tag/fusion/marker
Author Name: BD Biosciences Clontech
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
MCS MCS restriction enzyme-type multiple cloning site 45 129
bacterial origin ColE1 ColE1 (pUC-type) origin of replication 4111 4574
negative selection marker SacB SacB negative selection marker gene (sucrose sensitivity) 1057 2972
primer site M13 primer M13 primer sequencing site 4839 4855
primer site T7 primer T7 RNA Pol primer sequencing site 4865 4883
recombination site loxP site loxP recombination site 1015 1048
recombination site loxP site loxP recombination site 9 42
selectable marker CmR chloramphenicol resistance gene ORF (functional only after recombination of insert into vector with promoter) 986 327
selectable marker AmpR ampicillin resistance gene 3103 3963
splice donor site SD site splice donor site 132 136
tag HN 6xHN epitope tag 141 176

 

  • Gene
  • Pathways
  • Protein
  • Clone
  • Organism
  • Genome
  • Reagents

Gene

 

Gene Symbol : CD82
Symbol Nomenclature : CD82
SYNONYM : 4F9; C33; GR15; IA4; KAI1; R2; SAR2; ST6; TSPAN27
Designation : C33 antigen|CD82 antigen|inducible membrane protein R2|kangai 1 (suppression of tumorigenicity 6, prostate; CD82 antigen (R2 leukocyte antigen, antigen detected by monoclonal and antibody IA4))|metastasis suppressor Kangai-1|tetraspanin-27|tspan-27
Full Nomenclature : CD82 molecule
GENEID : 3732
GI : 61356912
GenBank Accession : AY888361
HGNC : 6210
MIM : 600623
Target GenBank: NM_002231

 

Reference Sequence Alignment

  

HsCD00000701      ATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAACTTGATCTTC
NM_002231.3       ATGGGCTCAGCCTGTATCAAAGTCACCAAATACTTTCTCTTCCTCTTCAACTTGATCTTC
                  ************************************************************

HsCD00000701      TTTATCCTGGGCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGACAAGAGCAGT
NM_002231.3       TTTATCCTGGGCGCAGTGATCCTGGGCTTCGGGGTGTGGATCCTGGCCGACAAGAGCAGT
                  ************************************************************

HsCD00000701      TTCATCTCTGTCCTGCAAACCTCCTCCAGCTCGCTTAGGATGGGGGCCTATGTCTTCATC
NM_002231.3       TTCATCTCTGTCCTGCAAACCTCCTCCAGCTCGCTTAGGATGGGGGCCTATGTCTTCATC
                  ************************************************************

HsCD00000701      GGCGTGGGGGCAGTCACTATGCTCATGGGCTTCCTGGGCTGCATCGGCGCCGTCAACGAG
NM_002231.3       GGCGTGGGGGCAGTCACTATGCTCATGGGCTTCCTGGGCTGCATCGGCGCCGTCAACGAG
                  ************************************************************

HsCD00000701      GTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCCTGATCCTCATTGCCCAGGTG
NM_002231.3       GTCCGCTGCCTGCTGGGGCTGTACTTTGCTTTCCTGCTCCTGATCCTCATTGCCCAGGTG
                  ************************************************************

HsCD00000701      ACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGATGGGCGGCATC
NM_002231.3       ACGGCCGGGGCCCTCTTCTACTTCAACATGGGCAAGCTGAAGCAGGAGATGGGCGGCATC
                  ************************************************************

HsCD00000701      GTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCAGGATGCCTGG
NM_002231.3       GTGACTGAGCTCATTCGAGACTACAACAGCAGTCGCGAGGACAGCCTGCAGGATGCCTGG
                  ************************************************************

HsCD00000701      GACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAACTGGACAGAC
NM_002231.3       GACTACGTGCAGGCTCAGGTGAAGTGCTGCGGCTGGGTCAGCTTCTACAACTGGACAGAC
                  ************************************************************

HsCD00000701      AACGCTGAGCTCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGAAGTCAAGGGG
NM_002231.3       AACGCTGAGCTCATGAATCGCCCTGAGGTCACCTACCCCTGTTCCTGCGAAGTCAAGGGG
                  ************************************************************

HsCD00000701      GAAGAGGACAACAGCCTTTCTGTGAGGAAGGGCTTCTGCGAGGCCCCCGGCAACAGGACC
NM_002231.3       GAAGAGGACAACAGCCTTTCTGTGAGGAAGGGCTTCTGCGAGGCCCCCGGCAACAGGACC
                  ************************************************************

HsCD00000701      CAGAGTGGCAACCACCCTGAGGACTGGCCTGTGTACCAGGAGGGCTGCATGGAGAAGGTG
NM_002231.3       CAGAGTGGCAACCACCCTGAGGACTGGCCTGTGTACCAGGAGGGCTGCATGGAGAAGGTG
                  ************************************************************

HsCD00000701      CAGGCGTGGCTGCAGGAGAACCTGGGCATCATCCTCGGCGTGGGCGTGGGTGTGGCCATC
NM_002231.3       CAGGCGTGGCTGCAGGAGAACCTGGGCATCATCCTCGGCGTGGGCGTGGGTGTGGCCATC
                  ************************************************************

HsCD00000701      ATCGAGCTCCTGGGGATGGTCCTGTCCATCTGCTTGTGCCGGCACGTCCATTCCGAAGAC
NM_002231.3       ATCGAGCTCCTGGGGATGGTCCTGTCCATCTGCTTGTGCCGGCACGTCCATTCCGAAGAC
                  ************************************************************

HsCD00000701      TACAGCAAGGTCCCCAAGTACTAG
NM_002231.3       TACAGCAAGGTCCCCAAGTACTGA
                  **********************..

Pathways

NCI : Direct p53 effectors
NCI : Validated nuclear estrogen receptor alpha network
Panther : p53 pathway

Protein

UniProt : Q7Z5N2
UniProt : E9PC70
UniProt : P27701
HPRD : 09004

Clone

Cloning Information : 20067
HIP Master Clone ID : 36163
Original Clone ID : FLH020067.01X

Organism


TAX_ID : 9606
Species Specific ID: 3732

Genome

Chromosome : 11
Map Location : 11p11.2

Reagents

Labome : CD82-antibody