ccdc50 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ccdc50
Gene Name:  coiled-coil domain containing 50
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTTGGTATAATCATCATCGTAATC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGACTGAAATCAACATTGATCAG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708499
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 825nts
Open reading frame: 1 to 825

ATGACTGAAATCAACATTGATCAGTCTAAGCTACCGGGAGTCAAGGAAGTTTGCAGAGAT
TTTGCAGTGCTGGAGGATCATACTCTTGCCCACAACCTGCAGGAACAGGAGATCGAGCAT
CACTTGGCAACGAATATACAGAGGAATCGTTTGGTAAAGAATGATTTACAAGTGGCCAAG
CAACTCCAAGAAGAAGAAGAACTCAGAGCACAAGCCCGGAAGAAAAATCATCACAATGAA
TTGGAAGTTTTAGATTCTGAAATAGCTCAGGAAATTCAAGATAGATTGGTAATTGAAGCA
AAACTCAGGCGTCAGCAAGAGGAGAAGGATGAGGACATTGCTCGACTATTACAAGAGAAA
GAGGAAAGGAGAAGAAAGAAGCATTACCCACCTACCCAAGAGGAACCTTATTACAAAGAC
AGAGAAGGGTTTCGACCAAAGGGGGTACAAGATGCACCTATATCTAAACACACAACAGGG
TTAAGAAATCAAGAGGAACACGATGCGCACCTTGCCCGGAGGCTTCAGGAAAAAGAATTC
AGGGCAAACGTTATGGATAAACGAGCAGCGCAGATGGCTCAGGATGAGGAAATTGCCCGC
TATCTTATGGACAAAGAAGAAAAAGCTTATAAGAAGTCAAAAGGACGTGAAAAAATGACA
GATGCTAAGCGACTAGAAGAATTGGAGGCACCTGATCATGTTAGGCAAAGGACAAGAGAA
GGTCAGGACCATCATCACCGTTCCAGAAGTGATAAACCATACAGGCCCCTTCCTCCACCT
CCAGATACTGCTGACGATGATGATTACGATGATGATTATACCAACTAA

Protein sequence predicted based on nucleotide sequence

MTEINIDQSKLPGVKEVCRDFAVLEDHTLAHNLQEQEIEHHLATNIQRNRLVKNDLQVAK
QLQEEEELRAQARKKNHHNELEVLDSEIAQEIQDRLVIEAKLRRQQEEKDEDIARLLQEK
EERRRKKHYPPTQEEPYYKDREGFRPKGVQDAPISKHTTGLRNQEEHDAHLARRLQEKEF
RANVMDKRAAQMAQDEEIARYLMDKEEKAYKKSKGREKMTDAKRLEELEAPDHVRQRTRE
GQDHHHRSRSDKPYRPLPPPPDTADDDDYDDDYTN

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable