itpa (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  itpa
Gene Name:  inosine triphosphatase (nucleoside triphosphate pyrophosphatase)
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAACCTTCGTGCCATTCTGGAT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCAGCTGCAGCCGGCA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00708867
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 585nts
Open reading frame: 1 to 585

ATGGCAGCTGCAGCCGGCAGGAGTATAGTCTTTGTAACCGGGAACGCTAAAAAGCTGGAA
GAGGTTGTTCAGATTCTTGGAGACAAATTTCCATGCAAATTAGTAGCCAAGAAAATTGAC
CTGCCAGAGTATCAAGGAGAACCAGATGAGATTTCCATTCAGAAATGTAGAGAGGCAGCA
AAACAGATTCAAGGCCCAGTTATTGTAGAGGATACTTGCTTGTGTTTTAATGCATTGGGA
GGTCTTCCTGGACCCTATATAAAATGGTTTCTTGAGAAGATCAAGCCAGAAGGGCTGCAC
CGAATGTTAGAAGGATTTGAGGACAAGTCAGCCATTGCCCTATGCACGTTTGCTTATTGC
AATGGAAATCCTGATGATACAGTTCTTCTATTTAGAGGCAAAACCTTGGGACAAATTGTA
CTTCCCAGAGGCCCAAGGGACTTTGGATGGGATCCTTGTTTCCAGCCGGATGGGTTTCAA
CAAACTTATGCTGAGCTCCCTAAAGAAGTGAAGAATACAATCTCCCACCGGTATCGTGCT
TTAAAAGAAATGTCGGACTACTTCATCCAGAATGGCACGAAGGTTTGA

Protein sequence predicted based on nucleotide sequence

MAAAAGRSIVFVTGNAKKLEEVVQILGDKFPCKLVAKKIDLPEYQGEPDEISIQKCREAA
KQIQGPVIVEDTCLCFNALGGLPGPYIKWFLEKIKPEGLHRMLEGFEDKSAIALCTFAYC
NGNPDDTVLLFRGKTLGQIVLPRGPRDFGWDPCFQPDGFQQTYAELPKEVKNTISHRYRA
LKEMSDYFIQNGTKV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable