meis3-a (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  meis3-a
Gene Name:  Meis homeobox 3
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTGAGCGTGAATGTCTATGT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCACAAAGGTATGATGAGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709500
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1359nts
Open reading frame: 1 to 1359

ATGGCACAAAGGTATGATGAGATGCTGCATTACCCAACGTTGGATGGGATGCCTCTGGCA
GGATTTGGGGATGCACACACAGGCCGAGCACTACAGCACCACTCACTTAGCCAGAGTGCA
CCCTATGGATCTACAGGAGCAGGTCACCGAGTACCAATGCCCCCTGGAATGGGCAGCAAC
GATGGTCTGAAAAGAGAGAAGGATGAGATTTATGGGCACCCACTGTTTCCTCTGCTGGCT
TTGGTGTTCGAGAAGTGCGAACTGGCTACGTGTTCCCCTCGTGACAATTCAGGGTCATTC
CCAGGGGGCGATGTTTGCTCTTCAGATTCTTTTAATGAAGATATTGCTGTTTTTGCCAAA
CAGGTCAGAACAGAAAAACCCCTGTTCTCTTCCAACCCAGAGCTGGACAGTTTGATGATT
CAAGCCATTCAAGTTTTACGATTCCACCTGTTGGAGTTAGAAAAGGTTCATGATCTGTGT
GACAATTTCTGCCACCGGTATATCACATGTCTAAAAGGCAAGATGCCCATTGATTTAGTG
ATAGATGATCGTGATGGCTCTTCCAAGTCTGATCTGGAAGATTTCACAGGTTCCTGCACT
AGCCTATCAGACCAGAATAACTCATGGCTACGAGATCATGATGAGACAGGCTCTGCTCAC
TCAGGTACACCGGGTCCTTCCAGCGGAGGACTAGCTTCCCAAAGTGGCGATAATTCCAGT
GAACAGGGTGATTGTATGGATAACAGTGTGGCATCACCCAGCACGGGGGATGATGATGAC
TTAGATAGAGACAAGAAGAGAAACAAGAAAAGAGGCATTTTCCCCAAAGTTGCCACGAAT
ATAATGAGGGCGTGGCTCTTTCAGCACCTCTCGCACCCTTATCCCTCTGAAGAACAGAAA
AAGCAGCTAGCGCAGGATACAGGGCTCACGATATTACAGGTTAACAACTGGTTTATTAAT
GCAAGAAGACGCATTGTCCAGCCTATGATTGACCAATCTAATCGCACAGGGCAAGGAGGT
GCTCCCTACAGTCCAGATGGTCAGAACATGGGAGGATATGTCATGGATGGACAACAACAC
ATGGGCATCCGACCCCCTGGCTTCCAAGGAATTCCTGGGGATTACACAGCAGCCCCAAGC
ACTATGCCCATGGGATTCCCACCGGCTGGGTACACACCTGCCATACCGCCACACTCCGCC
GGTCTTCGCCACGGACCGTCTCTACACTCCTACCTCCCAGGGCACCCTCACCACGCCTCT
ATGATTCTGCCAGCTGGCGCCTCCCCTCACCACCTGGTATCAGCGCAGAGTCCGGCGGAC
GCACTTCTAAATGGTCAAAACATAGACATTCACGCTCACTAA

Protein sequence predicted based on nucleotide sequence

MAQRYDEMLHYPTLDGMPLAGFGDAHTGRALQHHSLSQSAPYGSTGAGHRVPMPPGMGSN
DGLKREKDEIYGHPLFPLLALVFEKCELATCSPRDNSGSFPGGDVCSSDSFNEDIAVFAK
QVRTEKPLFSSNPELDSLMIQAIQVLRFHLLELEKVHDLCDNFCHRYITCLKGKMPIDLV
IDDRDGSSKSDLEDFTGSCTSLSDQNNSWLRDHDETGSAHSGTPGPSSGGLASQSGDNSS
EQGDCMDNSVASPSTGDDDDLDRDKKRNKKRGIFPKVATNIMRAWLFQHLSHPYPSEEQK
KQLAQDTGLTILQVNNWFINARRRIVQPMIDQSNRTGQGGAPYSPDGQNMGGYVMDGQQH
MGIRPPGFQGIPGDYTAAPSTMPMGFPPAGYTPAIPPHSAGLRHGPSLHSYLPGHPHHAS
MILPAGASPHHLVSAQSPADALLNGQNIDIHAH

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable