slc1a3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  slc1a3
Gene Name:  solute carrier family 1 (glial high affinity glutamate transporter), member 3
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACATTTTCGTTTCGCTGTCTAAGG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGACCAAAAGCAACGGGGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00709566
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1626nts
Open reading frame: 1 to 1626

ATGACCAAAAGCAACGGGGAAGATCCACGGTCAGGGAACAGGATGGAGCGCTTCCAGGAA
GGGGTTCGCCAGCGTTCTCTGATGGCCAAAAAAAAGATGCAGAACATAACAAAAGATGAT
GTGAAGAGTTTCTTCAAGCGCAATGGCTTTGTCCTTTTTACCGTCTTCGCTGTCATTATT
GGTATCATCCTGGGATTTTCTGTCCGAAAGTATCACATGACGTTCAGAGAGATCAAGTAC
TTCTCCTTCCCAGGGGAGCTTCTCATGAGAATGCTGCAGATGCTGGTCCTCCCTCTGATT
GTATCCAGCTTAGTGACAGGAATGTCTGCTCTCGACAGTAAGGCATCGGGAAAGATGGGA
ATACGAGCCGTGGTATATTACATGACAACAACGGTGCTTGCTGTTTTCATCGGTATTATT
ATTGTCATAATAATCCACCCAGGAAAGGGCAGCAAGGAGAAAATGCACAGAGAAGGAAAA
ATTGAGCAAGTAACTGCAGCTGATGCTTTTCTGGATTTAATAAGAAATATGTTCCCGCCT
AACATGGTAGAAGCCTGCTTTAAACAGTTCAAGACAAACTATGAGAAGAAACTGTTTCGA
GTCCCCATTCCAGCCAATGAATCCCTTCTGTCTTCAGTAGCCAATAATGTATCCGAGGCC
ATGGAAACATTGGTTAAGATGAGGGAAGAGATGATACCCGTTCCAGGAGCAGTGAATGGA
GTCAATGCCCTTGGACTTGTGGTTTTCTCAATGTCCTTTGGGCTGGTCATTGGAAACATG
AAAGAACAGGGGAAAGCTTTGAAGGACTTTTTTGACTCCCTCAATGAAGCAATTATGAGA
TTGGTTGCTGTAATCATGTGGTACGCACCAATTGGTATCCTCTTTCTGATTGCTGGAAAG
ATTGCCGAGATGGAAGATATGGGGGTAGTTGGTGGGCAGCTTGGAATGTACACCATTACG
GTTATTATAGGACTGCTTATTCATGCTGTGTTCGTTTTACCGCTCCTCTACTTTTTGGTA
ACTCGGAAAAACCCATGGGTTTTCATTGGTGGATTGATCCAAGCCTTAATCACCGCTCTG
GGAACCTCCTCGAGTTCTGCTACATTACCAGTTACATTTAAATGCCTGGAAGAGAACAAT
CACGTGGACAAAAGAGTTACAAGATTTGTGCTACCAGTAGGAGCCACAATCAATATGGAT
GGCACTGCCCTCTATGAAGCCCTGGCTGCCATTTTTATCGCTCAAGTCAACAATTACGAT
CTGAATTTTGGGCAGATTATTACAATCAGTATCACAGCTACAGCCGCCAGCATTGGAGCA
GCGGGAATCCCTCAAGCTGGTTTGGTCACCATGGTTATTGTTTTGACATCAGTGGGGCTT
CCAACAGAAGACATTACGTTGATTATTGCCGTAGACTGGTTTTTGGACCGTCTTCGTACC
ACAACCAATGTACTTGGAGACTCTCTGGGTGCAGGCATCGTAGAACATTTGTCTAGGCAC
GAGCTCAAGGGAGGCGACGCTGAGATGGGCAACTCAGTCATTGAGGAGAATGAAATGAAG
AAACCATACCAGCTGATCTCCCAGGAAAATGAAAGCGAGAAACCCTTAGACAGCGAAACG
AAAATGTAA

Protein sequence predicted based on nucleotide sequence

MTKSNGEDPRSGNRMERFQEGVRQRSLMAKKKMQNITKDDVKSFFKRNGFVLFTVFAVII
GIILGFSVRKYHMTFREIKYFSFPGELLMRMLQMLVLPLIVSSLVTGMSALDSKASGKMG
IRAVVYYMTTTVLAVFIGIIIVIIIHPGKGSKEKMHREGKIEQVTAADAFLDLIRNMFPP
NMVEACFKQFKTNYEKKLFRVPIPANESLLSSVANNVSEAMETLVKMREEMIPVPGAVNG
VNALGLVVFSMSFGLVIGNMKEQGKALKDFFDSLNEAIMRLVAVIMWYAPIGILFLIAGK
IAEMEDMGVVGGQLGMYTITVIIGLLIHAVFVLPLLYFLVTRKNPWVFIGGLIQALITAL
GTSSSSATLPVTFKCLEENNHVDKRVTRFVLPVGATINMDGTALYEALAAIFIAQVNNYD
LNFGQIITISITATAASIGAAGIPQAGLVTMVIVLTSVGLPTEDITLIIAVDWFLDRLRT
TTNVLGDSLGAGIVEHLSRHELKGGDAEMGNSVIEENEMKKPYQLISQENESEKPLDSET
KM

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable