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xbp1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

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Explanation of Terms


Gene: Gene Symbol:  xbp1
Gene Name:  X-box binding protein 1
Sequence             
Original Clone ID: None
Keyword: None
Species: Xenopus laevis
Type: cDNA
Vector Name: pDONR223               Format:  FUSION
Source: Xenopus Gene Collection
Description: None
Comments: The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTTCATTAATGGCGTCCAG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGTGGTCGTGGGAGC, XGC Collection: IRBH
Mutation/
Discrepancy :
No / No
Publications: None
Authors: Aaron Zorn
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
David E. Hill
Michael J. Gilchrist
MRC National Institute for Medical Research
Todd Stukenberg
University of Virginia School of Medicine
Xenopus Gene Collection
XlCD00709817

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AVAILABLE
No restriction
Special MTA: None

 

Insert sequence: 762nts         Open reading frame : 1 to 762

ATGGTGGTCGTGGGAGCCCCCAAAGTGATCTTTATTCCCGGGAATCAGTGTGAGCAGGCC
GACGCCTTGGGATCCGTAATGTTACCCATAAGCAGCCCGAGCAGTCCGGAGTCCGTCAGC
AATGAGCAACCCCCAAGGAAGAGACAGAGACTCACCCACCTTAGCTCGGAGGAAAAAGCG
CTTAGAAGAAAATTAAAAAACAGAGTAGCAGCACAGACTGCTCGGGACAGGAAAAAAGCC
AGAATGAGTGAACTGGAACAGCAAGTGATAGATTTAGAAATGGAGAATGAGAAGCTTTTA
ATTGAAAACCAGATTTTAAGAGAAAAATCTCATGGCTTGTTGACTGAAAACCAGGAGCTA
CGCCAAAGATTGGGCCTAAGCACCCTGGAGTTAAAGAAAGAAGAAGAAGAAGAATCACAG
GAGCTGAATCAGTCCAGAAAAGATAAAGTCAGGCCGGAGACCGGGTCCGCTGAGTCCGCA
GCACTCAGACTATGTGCCCCTCTGCAGCAGGAGCAGGCCCAGATGTCTCTGAACCTGACA
GTGTCTACATGGATCCTGACAGCCCTGACACTTCAGACTCTGAGTCTGATCTCTTGTTGG
GCCTTCTGGAAAGCCTGGACTCAGACTTGTTGCTTGCCTATGAAGGAGCACTTGCTGGAA
GCCCAGATGAAGAGCTCAAGGGAGAAGAATCAGATTCCATATCCTCCTCCCCATCTTCTC
CTGTGGGGACCCCATCAGCCAAGCTGGACGCCATTAATGAACTGA


Coding Sequence Details
Insert Sequence Verified?: Y Verification Method: End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence: None
3' Linker Sequence: None

 

 Recommended Growth Condition:

Distributed in bacterial strain : DH5-alpha T1 phage resistant
Antibiotic Selection: Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition:  100 ug/mL spectinomycin
Growth Condition:  Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:  None
Protein Expression Results: None
Recommended expression in: Not Applicable
       DyNA Vector Map

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Vector Name: pDONR223

pDONR223 in advanced viewed

Synonyms: None
Sequencing Primer: Forward:  M13F
Reverse:  M13R
Description: Recombinational donor/master vector; spectinomycin resistance; recombinational cloning.
Comments: pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp): 5005
Parent Vector: None
Empty Vector: None
Properties: Gateway, donor (entry), recombinational cloning
Author Name: Invitrogen
Publications: None
Vector Map:         Vector Sequence:
Sequence:

 



Vector Features:

Type Name Description Start Position End Position
bacterial origin pBR322 origin pBR322 bacterial origin 4339 4958
death cassette ccdB ccdB negative selection gene (death cassette), LOST in recombined (with insert) form 1200 1409
primer M13 forward M13 forward sequencing primer 536 553
primer M13 reverse M13 reverse sequencing primer 3020 3040
recombination site attL1 attL recombination site 1 present in recombined (with insert) form only 569 652
recombination site attL2 attL recombination site 2 present in recombined (with insert) form only 2896 2983
selectable marker SpectR spectinomycin resistance gene 3471 4262
trxn termination sequence rrnB rrnB T1 and T2 terminator 268 470

 

  • Gene
  • Clone
  • Organism

Gene

Target GenBank: BC110723

 

Clone

Cloning Information : 762753
XGC Gene Symbol : xbp1
Xenbase ORF Name : xbp1 ORF2

Organism


Species Specific ID: 380215