haus3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACGCAGAAGAACCGCCGCC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAGCGGGGGGGACCG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710304
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1791nts
Open reading frame: 1 to 1791

ATGAGCGGGGGGGACCGGTTTGTACAAACGCTGCAGAAGCTCAATTATCCTAAAGGCGCC
CAGCTGGACGGGGAGGATTTTGATTGGCTCTTTGAAGCAGTCGATTTGAAACCTTTCCTA
GACTGGTTTTGTTCTGCCGCAAGTGAACAGAATGTGGTGCCAGACGAGAAGCTACAAGCT
TTTAATACACTCAAGGAGTCGGGGAAACCTGTACTGGATGAAAAGGCTTTGGATGAAGTA
CTAAAAACCTTCTCCATATCAAAAGTACCCGCCATAGAAGAAGTGGCAATTGAGAAACTG
GAGGAAGAAGTTAAAGCTTTGCAGAAGCAGAAAAATCTACATATCAGGAGGCGGAATAAG
CTGCAGATGGTGGAGTCTGGTAACCGCCAAATGTGCTTGAAATCTAAAGATAAGGAAGAA
GAGACTGGCAGAGCTTTCCAAGAAGTTCTACATCTCCTCCGGGTCACCAACAAGAAGCTG
AATCATGAGCTGCAGAGCATCGTTAATGGTGTCCAGACCCTAATGTCCTTCTTTTCCACT
CCAGAAACAGCCTGCGAGCTGTCATCTCAGCCCATATTTCTGTCTCAGCTCCTCCTTGAT
AAGTACCTGTCCCTTGAAGAACAAAGCACAGCCGCTCTTACCTCCTTTACTAAGGAACAT
TTTTTTGAGGGCATGTCTAAATTTGTTGAAGGCTCTGACGAGAACTTTCAGCTTGTCCAG
CTGAATGTGAACAGTTTCGGGGAAGATGGCACCACCGAGGACAAGTGCAAAGAGATGATG
AGACTTCAGTTGGCGTATATTTGTGCCAAGCACAAGCTGATTCAAATGAAGGCTAAAAGT
GCCAGTTTGAAAGTCGGCCTACAGTGGGCTGAAAATAATGCCAGTGTCGTGCAGGATAAG
GCCTCTCAGAAAGAAGAAAATCTCAAAGTAAGAATCACAAGCTTAAAAAACGAGACTTTG
CAAATTGAGAATCATACAAATAGCATTAGCAATGAGAAGCTCCCTGGCTTGGTGCGGGAC
AATGCCCAGCTATTAAATATGCCCATTGTGAAAGGAGACTATGACCTGCAGATGGCACAT
CAGACCTCATGTTCCTCTAGACAAGACCTGGTGTGTGACCATCTCATGAAACAGAAAGCT
TCCTTTGAACTCCTTCAGCTGGGCTACGAGCTGGAACTAAGGAAGCACAGAGATGTGTAT
CGGGAGCTGGGAAGCATCGTACAGGAACTGAAGGAAAGCGGTGATAAACTGGAGGAAAGG
TTAACAATGTTATCCGATGTAAACTTGCTGTCAGCCTCCAAACCCAGGAGCAATATTGAT
TCTAAGGATTTGACTTCACACAGATTATACCAGCTGCTGGATGGAGACAACACCCAGAAA
CTGTTCAGAACATATGACGGTTTGGAATCGGTGGCCCAGAAGTTAAGCCAAGACATTGCT
TCCATGAGAGATCAGCTGGAGGTTTCTGAGCAAGAGCATTCCCTTCTCTTGTCCAAACTG
GACTCTCATCTGAAGGAGCTCCGTGACTTTATGTATCCTGAAGGAAACACTCTTATGTTA
ACCACCCCGGAGCTTTCAGGTGAGTTCCATCAGTTAGGGTCCCAGCTGGAGAAACTAAAC
CACATTACAGTGGAAATCCTGGGAGATCTGCAGCTGAAGAGGAAGATGCTGGAGTCCAAT
AAGTTGCAGCAGATAGAGAAGCAGTTGTACGTCTATTTCTTCCAAAACGAAGAGCAACTA
AAAAGCATTGTAGGAAAACTGGAGGCCCAGACAGGCGGCGGTTCTTCTGCGTGA

Protein sequence predicted based on nucleotide sequence

MSGGDRFVQTLQKLNYPKGAQLDGEDFDWLFEAVDLKPFLDWFCSAASEQNVVPDEKLQA
FNTLKESGKPVLDEKALDEVLKTFSISKVPAIEEVAIEKLEEEVKALQKQKNLHIRRRNK
LQMVESGNRQMCLKSKDKEEETGRAFQEVLHLLRVTNKKLNHELQSIVNGVQTLMSFFST
PETACELSSQPIFLSQLLLDKYLSLEEQSTAALTSFTKEHFFEGMSKFVEGSDENFQLVQ
LNVNSFGEDGTTEDKCKEMMRLQLAYICAKHKLIQMKAKSASLKVGLQWAENNASVVQDK
ASQKEENLKVRITSLKNETLQIENHTNSISNEKLPGLVRDNAQLLNMPIVKGDYDLQMAH
QTSCSSRQDLVCDHLMKQKASFELLQLGYELELRKHRDVYRELGSIVQELKESGDKLEER
LTMLSDVNLLSASKPRSNIDSKDLTSHRLYQLLDGDNTQKLFRTYDGLESVAQKLSQDIA
SMRDQLEVSEQEHSLLLSKLDSHLKELRDFMYPEGNTLMLTTPELSGEFHQLGSQLEKLN
HITVEILGDLQLKRKMLESNKLQQIEKQLYVYFFQNEEQLKSIVGKLEAQTGGGSSA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable