prickle1-b (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGAAATAATGCAGTTTTTGCCTTT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTTGCCCAGGACATCTGAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710311
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2598nts
Open reading frame: 1 to 2598

ATGTTGCCCAGGACATCTGAACTCCGGTACTCAGCTACATGCAACTTGCTCTCCTCACCA
AGAGCAATTGATGAGCTGGAACGACCCATAACAATCTTACACATGTCGTTGGAAATGGAT
CAGAAAGTTAACAAGCTTACATTTGGGTGTCAGCGAAGTTCCACCTCAGATGATGACTCT
GGTTGCGCCATGGAAGAATACACGTGGGTTCCACCAGGTCTTCGGCCCGAACAGGTCCAG
TTATACTTCGCTTGCCTGCCTGAAGAGAAGATTCCATATGTAAACAGCATTGGTGAGAAG
TATAGGATCAAGCAACTCTTGTACCAACTTCCTCCACATGACAATGAGGTGCGATATTGT
CAGTCGTTGTGTGAAGAAGAGAAGAAGGAGTTACAGATGTTTAGTGGACAACGCAAAAAG
GAGGCTCTTGGCAGAGGAAATATTAAAATGTTGTCACGGGCTGTGATGCATGCTATGTGT
GAAAAGTGCGGAGAGAAGATTAATGGAGGTGAAATTGCAATTTTTGTTTCAAGAGCTGGC
CCGGGGGTGTGCTGGCACCCATCATGCTTTGTTTGTTCCACATGTAACGAGCTACTTGTT
GATCTAATCTACTTCTACCAAGATGGAAAAATACACTGTGGAAGGCACCATGCTGAACTA
CTTAAGCCAAGATGCTCAGCTTGTGATGAGATAATATTTGCAGATGAATGCACTGAAGCT
GAAGGTCGCCACTGGCACATGAACCATTTCTGTTGCTACGAGTGTGAAACGGTGCTGGGG
GGACAGAGATACATCATGAAAGATGGTCGCCCATTCTGCTGCGGTTGCTTTGAATCTCAT
TACGCAGAGTACTGTGAATCCTGCGGAGACCATATAGGAGTGGATCACGCACAAATGACC
TACGATGGGCAACACTGGCATGCTACAGAGACATGTTTTTCTTGCGCCCAGTGTAAAGTT
TCTCTTTTGGGTTGCCCTTTCTTGCCCAAAAAGGGGCGGATTTATTGTTCCAAGGCATGC
AGTTTGGGAGAGGATGTGCATGCATCTGACTCTTCTGACTCTGCCTTTCAATCTGCAAGA
TCGAGAGAATCCAGAAGAAGTGTACGGATGGGCAAAAGTAGTAGATCTGCAGACCAGTGT
AGACAATCTCTGCTATTGTCTCCTGCTTTAAATTACAAGTTCCCAGGAATGTCTGGCAAT
GCTGACGATACTCTGTCTAGGAAGATGGATGATCTCGGTATATCAAGGCAGGGAGCAGGT
TTTGATAATGACTTCTGGAAAGCAAGAGATGAGCAGGAGACTCCAGAGGACCATGAAGAA
TGGGCTGAACATGATGATTACATGACTCAGTTGCTTCTTAAATTTGGAGAAAAGGGTCTG
TTTCAGCAGCCACCTGAGGATAACAGATCTACTGAGCACTGGATGTCTGAGAATATCAAA
GGCAAAAATGATTTACAAAGAAACAACCGTAATAAAAGCTTAGCTAGTAAAAAATATCAG
AGTGATATGTACTGGACCCAGTCCCAGGATGGACTTGGAGATTCTGCATATGGTAGCCAT
CCTGGCCCCGCTAGCAGCCGAAAATTACAAGAACTGGATATGGATCATGGAGCTTCTGCT
TACATGCATGAGAAAATGCCATGGTATAAACGTTCATTGGAATGTTTGTCAGATAATCTG
AAACCTCAGAATGAGAACATCCGTGACTCTATGGATTCCTTAGCACTGTCTAATATAACA
GGAGCATCTGTGGATGGAGAAAACAAGTCCCGACCTTCACTATTTTGCTACCAGAATTTT
CAAGACTTGAACACCAGGGATTGTGAGAAAATGAGCAATATGGGAACTCTTAATTCATCC
ATGCTGAACAGAAGCACAGAGTCTCTGAAGAGTTTAACTTCTGAGATTTGCCAGGAAAAG
CCACCTCCAGAGGAAAAGCCAATGCACACGTCTGCGCTCAGGAGGTCTAAATCCCAAACA
AGACCTCAGGTCAAATTTTCAGATGATGTCATTGATAATGGAGACTATGGCAGCATAGAA
ATCCGTCAGCCGCCCATGAGCGAAAGGAGTCGGAGAAGAGTTTACAACTTTGAAGAGCGA
AGCCAAAGGCCTCATCATCACCGGCGACGGAAAAGCAGGAAATCACGTTCTGAAAATGCA
CTTCATCTTGCGACAGATAGCAAGCCGTCTGGAAAAGAAAGAAACCGATTTTACACAGCA
GAGGATTACGAGAGACTTTTTCATAATAAATCTGCTCATCAGGTTCAGGCGTATATTCAG
AACGCTGACCTCTTTGGACAATATTCAAATGCTGCTTCTAATGTTGGACTGCCCAGCAAA
GTTGCAGGTAAATTCCTTGGGTTATATGGTGAAGATGAAGATTCTTGGTGCTCAACCTGT
TCCTCGTCCTCTTCGGATTCTGAAGAGGAGGGCTATTTCCTTGGCCAGCCTATTCCTAAG
CCCCGGCCGCAGAGATATCAGTATTTTTCCGATGACTTGTGTAGTCCAACAAATGCGCTA
TCCAGTTCTCAGTTTAGTCAAAGGACCACTAAATCCAAGAAGAAAAAGGGGCACAAAGGC
AAAAACTGCATTATTTCCTAA

Protein sequence predicted based on nucleotide sequence

MLPRTSELRYSATCNLLSSPRAIDELERPITILHMSLEMDQKVNKLTFGCQRSSTSDDDS
GCAMEEYTWVPPGLRPEQVQLYFACLPEEKIPYVNSIGEKYRIKQLLYQLPPHDNEVRYC
QSLCEEEKKELQMFSGQRKKEALGRGNIKMLSRAVMHAMCEKCGEKINGGEIAIFVSRAG
PGVCWHPSCFVCSTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEA
EGRHWHMNHFCCYECETVLGGQRYIMKDGRPFCCGCFESHYAEYCESCGDHIGVDHAQMT
YDGQHWHATETCFSCAQCKVSLLGCPFLPKKGRIYCSKACSLGEDVHASDSSDSAFQSAR
SRESRRSVRMGKSSRSADQCRQSLLLSPALNYKFPGMSGNADDTLSRKMDDLGISRQGAG
FDNDFWKARDEQETPEDHEEWAEHDDYMTQLLLKFGEKGLFQQPPEDNRSTEHWMSENIK
GKNDLQRNNRNKSLASKKYQSDMYWTQSQDGLGDSAYGSHPGPASSRKLQELDMDHGASA
YMHEKMPWYKRSLECLSDNLKPQNENIRDSMDSLALSNITGASVDGENKSRPSLFCYQNF
QDLNTRDCEKMSNMGTLNSSMLNRSTESLKSLTSEICQEKPPPEEKPMHTSALRRSKSQT
RPQVKFSDDVIDNGDYGSIEIRQPPMSERSRRRVYNFEERSQRPHHHRRRKSRKSRSENA
LHLATDSKPSGKERNRFYTAEDYERLFHNKSAHQVQAYIQNADLFGQYSNAASNVGLPSK
VAGKFLGLYGEDEDSWCSTCSSSSSDSEEEGYFLGQPIPKPRPQRYQYFSDDLCSPTNAL
SSSQFSQRTTKSKKKKGHKGKNCIIS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable