prmt1-b (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  prmt1-b
Gene Name:  protein arginine methyltransferase 1
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAACGCATTCTATAGTCTGTCGAAC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGAGGTTTCTTGTGCTCTG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710593
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1029nts
Open reading frame: 1 to 1029

ATGGAGGTTTCTTGTGCTCTGCCTGAGGGCAGTGTGAAGCCAAATGCAGAAGACATGACA
TCAAAAGATTACTACTTTGACTCGTATGCTCACTTTGGCATTCATGAGGAGATGTTAAAA
GATGAAGTTCGGACACTGACCTATCGCAACTCAATGTTTCACAACAGACATCTATTTAAA
GATAAAGTTGTGTTGGATGTTGGGAGTGGCACGGGTATTCTCTGCATGTTTGCAGCAAAG
GCTGGTGCCAAGAAAGTCATTGGGATTGAGTGCTCAAGTATATCTGACTATGCCATAAAA
ATCGTGAAGGCAAATAAACTAGATCACGTGGTCACCATCATCAAAGGCAAAGTAGAAGAG
GTGGAGCTCCCAGTAGAAAAAGTGGACATCATAATCAGTGAATGGATGGGATATTGTTTA
TTCTATGAATCCATGCTTAACACAGTTATCTATGCACGGGATAAATGGCTGAACCCAGAT
GGATTAATATTTCCAGATCGTGCTACACTGTATGTAACTGCTATTGAGGATAGACAATAC
AAAGACTACAAGATTCACTGGTGGGAAAATGTTTATGGCTTTGATATGTCATGCATTAAG
GATGTAGCCATCAAGGAGCCTCTGGTTGATGTTGTTGACCCCAAGCAACTGGTCTCAAAC
GCCTGCCTTATAAAGGAAGTGGATATTTATACTGTCAAAGTTGACGACTTGACCTTTACC
TCTCCATTCTGCCTGCAAGTGAAACGAAATGACTACATCCATGCCATGGTTGCGTATTTC
AACATTGAATTCACACGGTGCCATAAAAGAACTGGCTTTTCTACAAGTCCTGAGTCTCCG
TACACTCACTGGAAGCAAACCGTTTTCTACATGGAAGACTATCTCACTGTTAAGACTGGC
GAGGAGATCTTTGGTACCATAAGCATGAAGCCTAATGCCAAAAACAACCGTGACCTGGAT
TTTACTGTTGACATTGATTTCAAGGGCCAGCTGTGCGAGTTGTCCTGTTCGACAGACTAT
AGAATGCGTTGA

Protein sequence predicted based on nucleotide sequence

MEVSCALPEGSVKPNAEDMTSKDYYFDSYAHFGIHEEMLKDEVRTLTYRNSMFHNRHLFK
DKVVLDVGSGTGILCMFAAKAGAKKVIGIECSSISDYAIKIVKANKLDHVVTIIKGKVEE
VELPVEKVDIIISEWMGYCLFYESMLNTVIYARDKWLNPDGLIFPDRATLYVTAIEDRQY
KDYKIHWWENVYGFDMSCIKDVAIKEPLVDVVDPKQLVSNACLIKEVDIYTVKVDDLTFT
SPFCLQVKRNDYIHAMVAYFNIEFTRCHKRTGFSTSPESPYTHWKQTVFYMEDYLTVKTG
EEIFGTISMKPNAKNNRDLDFTVDIDFKGQLCELSCSTDYRMR

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable