acta2 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  acta2
Gene Name:  actin, alpha 2, smooth muscle, aorta
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGAAGCATTTGCGGTGAACAATGGA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTGTGACGAAGAAGACACTA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710624
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1131nts
Open reading frame: 1 to 1131

ATGTGTGACGAAGAAGACACTACTGCACTGGTGTGTGACAATGGCTCTGGACTCTGCAAG
GCTGGATTTGCTGGGGACGATGCTCCAAGGGCCGTATTCCCCTCCATCGTGGGTCGCCCC
AGGCACCAGGGTGTGATGGTTGGTATGGGTCAGAAGGACAGCTATGTTGGTGATGAAGCT
CAGAGCAAGAGAGGTATTCTGACACTGAAATACCCAATTGAACACGGCATCATTACAAAC
TGGGATGACATGGAGAAGATCTGGCACCACAGCTTCTACAATGAATTACGAGTGGCCCCA
GAGGAACACCCAACCCTGCTCACTGAGGCTCCACTGAATCCCAAAGCTAACAGAGAAAAA
ATGACCCAGATAATGTTTGAGACATTTAACGTGCCAGCAATGTACGTTGCTATTCAGGCG
GTGCTATCCCTCTATGCTTCTGGCCGTACTACTGGTATCGTACTTGACTCTGGTGATGGT
GTCACCCACAATGTACCAATCTATGAAGGGTATGCTCTTCCTCATGCAATCATGCGTCTT
GACCTGGCTGGCCGTGACCTAACAGACTACCTCATGAAGATCTTGACTGAACGCGGCTAT
TCCTTTGTAACTACTGCTGAGCGTGAAATTGTGCGAGACATCAAGGAGAAGCTGTGCTAT
GTAGCCCTTGACTTTGAGAATGAAATGGCTACCGCTGCCTCTTCCTCCTCTCTGGAAAAG
AGTTATGAACTTCCCGACGGTCAGGTCATCACAATTGGAAATGAGCGTTTCCGCTGCCCC
GAGACCCTGTTCCAGCCATCTTTCATTGGTATGGAATCTGCCGGTATCCATGAAACCACT
TACAACAGCATCATGAAATGCGACATAGACATCCGAAAGGACCTGTATGCCAACAACGTA
CTGTCCGGTGGTACCACCATGTACCCTGGCATTGCTGACAGAATGCAGAAGGAGATCACT
GCTTTGGCCCCCAGCACAATGAAAATCAAGATCATTGCCCCACCTGAGCGTAAGTACTCC
GTCTGGATTGGTGGCTCCATCCTCGCCTCCCTGTCTACCTTCCAGCAGATGTGGATCAGC
AAACCCGAATACGACGAAGCCGGCCCCTCCATTGTTCACCGCAAATGCTTCTAA

Protein sequence predicted based on nucleotide sequence

MCDEEDTTALVCDNGSGLCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEA
QSKRGILTLKYPIEHGIITNWDDMEKIWHHSFYNELRVAPEEHPTLLTEAPLNPKANREK
MTQIMFETFNVPAMYVAIQAVLSLYASGRTTGIVLDSGDGVTHNVPIYEGYALPHAIMRL
DLAGRDLTDYLMKILTERGYSFVTTAEREIVRDIKEKLCYVALDFENEMATAASSSSLEK
SYELPDGQVITIGNERFRCPETLFQPSFIGMESAGIHETTYNSIMKCDIDIRKDLYANNV
LSGGTTMYPGIADRMQKEITALAPSTMKIKIIAPPERKYSVWIGGSILASLSTFQQMWIS
KPEYDEAGPSIVHRKCF

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable