tbx3-b (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  tbx3-b
Gene Name:  T-box 3
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAATCAGGGGAACCGCTCCTT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAATTTACCCATGAGAGACC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00710745
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2133nts
Open reading frame: 1 to 2133

ATGAATTTACCCATGAGAGACCCAGTAATTTCAGGGTCAGGCATGGCTTACCATCCATTC
CTATCCCACAGGGCACCAGACTTTGCAATGAGTGCTGTTCTTGGACACCAACCTCCCTTT
TTTCCAACCCTGGCTCTGCCACCCAATGGGGCTGCTCTTTCCCTTTCTAGTGCCCTGGCC
AAGCCCATCATGGATCAATTAATGGGTGCCGCAGAGACTGGCATCCCCTTTTCATCTTTG
GGTCATCAGGCAGCTGCTCATTTGAGGCCTCTTAAAACCCTTGAACCAGAAGAAGAAGTT
GAAGACGATCCAAAAGTCCACCTGGAAGCCAAGGATCTCTGGGAACAGTTCCACAAAAGG
GGAACAGAGATGGTCATCACAAAATCTGGGAGGAGAATGTTTCCTCCATTTAAAGTGAGG
TGCTCTGGTCTGGACAAGAAAGCCAAATACATTCTACTGATGGACATTGTGGCTGCAGAT
GACTGCAGGTACAAGTTCCATAACTCCCGATGGATGGTGGCTGGAAAGGCAGACCCCGAA
ATGCCCAAACGAATGTATATCCACCCAGACAGCCCGGCCACTGGGGAGCAATGGATGTCC
AAAGTAGTGAACTTCCATAAACTCAAACTGACCAACAATATCTCGGATAAACATGGATTT
ACTATTTTAAACTCCATGCACAAATACCAACCCAGGTTCCACATAGTGCGAGCTAATGAC
ATTCTGAAATTACCCTACAGCACATTCAGAACTTATGTGTTCCCTGAGACAGAATTCATT
GCAGTGACTGCATACCAGAATGATAAGATCACTCAATTAAAAATTGATAACAATCCCTTT
GCCAAAGGCTTTCGTGACACTGGGAATGGCAGGAGAGAAAAGAGGAAACAGTTGGCCTTG
CACTCCATGCGACCTTATGATGATCACCAGAAGAAGGATAATGGGAGTTCGGACGAGTCC
TCTAGTGAACAAACTGCCTATAAATGTTTTGGCCAGACTTCTTCTCCAACCGCACCTGCA
GTGGCCACCTCATCCCTCAAAGGTGTGCCCCCCAGTGACGGGGAGAGTGACGAGGAGAGT
AGGGATGAGGGAGGACCGGACACAGGCGAATCTGCCAAGATCTCCACAACAACCAGTGAG
GATTGCAGGGAGTTGGCCAGTCCCCCCAGGGCCCCGCTTTTCCCATCAGATGTGGGCAGC
AGCAGCGGCAGGAGCAAGGAGCAGAGCGGCAGGACAGGAAAAAGTTCCCCGGACTCCCGG
CACAGCCCAGGCCACATCACCTCCAGCAGCAGCAGCAGGATCCTGAGCATCGACCATTTG
AAAAGCCCAACCAGAGACTCCCATAAAACAGAGGAGCCCCAGCGCTCCAAGGAACGTTAC
CCCCCTCTCACTGTGCAAACAGACAGTAGCGCCCACCAGCTCAGCCAGGGCCATCTGCAC
AACCTTGGCTTCCCCCCAGGACTAACTGGGCAGCAGTTCTTTAACCCTCTGGGCGCCTCA
CACCCTCTACTCCTGCACCCAAGTCACTTTGCAATGGGAGGGGCTTTTTCCAATATGGCC
GCTGGCATGAACCCACTTCTGGCCTCAGTTTCAGGAGCATCACCTGGGATCAACAGCATA
GACTCTACCGTCATGGCATCTTCCTCTCAAGGGCTGGCGGGATCATCTGCACTGCCTTTC
CACCTGCAGCAGCAAGTCCTGGCCTCTCAGGGCCTGGCAATGTCTCCCTTCGGAGGGCTT
TTCTCCTACCCTTACACGTACATGGCTGCGGCTGCTGCAGCCTCCTCTGCTGTGCACAGA
CACCCTTTTCTTAACGCCATGCGCCCCAGGCTCAGGTACAACCCGTACTCTTTCCCTATG
CCCTTTGCAGAAAGCAACAGCCTCCTCACCACTGCCCTGCACTCTCTGCCCAACGGCGCG
CTGGATGGCAAAGCCAGTCTCCTCTCCAGTCCGGCCGCTACTGCTCTGGACAATGAACTG
ACCAGCAGATCCTCCACCCTGTCTTCTGGCTCCGTCTCTTTATCCCCAAAGCTGTGCCCA
GAGAAGGAAGCAAGCAGTGAGCTCCAAAATATTCAGCGGCTAGTGAGCGGGCTGGATTCT
AAGCAGGAGCTGTCAAGGAGCGGTTCCCCTGATTGA

Protein sequence predicted based on nucleotide sequence

MNLPMRDPVISGSGMAYHPFLSHRAPDFAMSAVLGHQPPFFPTLALPPNGAALSLSSALA
KPIMDQLMGAAETGIPFSSLGHQAAAHLRPLKTLEPEEEVEDDPKVHLEAKDLWEQFHKR
GTEMVITKSGRRMFPPFKVRCSGLDKKAKYILLMDIVAADDCRYKFHNSRWMVAGKADPE
MPKRMYIHPDSPATGEQWMSKVVNFHKLKLTNNISDKHGFTILNSMHKYQPRFHIVRAND
ILKLPYSTFRTYVFPETEFIAVTAYQNDKITQLKIDNNPFAKGFRDTGNGRREKRKQLAL
HSMRPYDDHQKKDNGSSDESSSEQTAYKCFGQTSSPTAPAVATSSLKGVPPSDGESDEES
RDEGGPDTGESAKISTTTSEDCRELASPPRAPLFPSDVGSSSGRSKEQSGRTGKSSPDSR
HSPGHITSSSSSRILSIDHLKSPTRDSHKTEEPQRSKERYPPLTVQTDSSAHQLSQGHLH
NLGFPPGLTGQQFFNPLGASHPLLLHPSHFAMGGAFSNMAAGMNPLLASVSGASPGINSI
DSTVMASSSQGLAGSSALPFHLQQQVLASQGLAMSPFGGLFSYPYTYMAAAAAASSAVHR
HPFLNAMRPRLRYNPYSFPMPFAESNSLLTTALHSLPNGALDGKASLLSSPAATALDNEL
TSRSSTLSSGSVSLSPKLCPEKEASSELQNIQRLVSGLDSKQELSRSGSPD

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable