snw1 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  snw1
Gene Name:  SNW domain containing 1
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACTCCTTCCTGCGCTTCT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCGCTAGCCAGTTTT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00711421
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1605nts
Open reading frame: 1 to 1605

ATGGCGCTAGCCAGTTTTTTGCCTGCTCCAACACAGTTATCACAGGATCAGCTAGAGGCA
GAAGAAAAGCTGAGAGCACAGAAATCTCGTCAGACAGCTCTAGTGTCATCACGGCGTGAA
CCACCTCCCTATGGCCACAGGAAAGGATGGGTTCCCAGATCCCTCGAGGATTTTGGAGAT
GGCGGTGCTTTTCCAGAGATACATGTGGCACAATACCCACTGGACATGGGGCGAAAAAAG
AAAATGTCCAATGCCTTGGCTGTGCAGGTGGATGCAGAAGGAAAAATAAAATATGATGCT
ATTGCTCGACAGGGACAATCCAAAGATAAGGTTCTTTTCAGCAAGTACACAGACCTGGTA
CCTAAAGAGGTGATGGATGAAGATGATCCTGAACTACAAAGACCTGATGAGGAAGCTATT
AAAGAGCTCACAGAGAAGACAAGACAGGCTCTGGACAAATCCGTGTCCCAGAAGATTGCT
GCAGCGATGCCTGTACGGGCTGCAGATAAACTGGCCCCTGCACAGTATATTAGGTACACA
CCCTCTCAACAGGGGGTGGCTTTTAACTCTGGTGCCAAACAGAGGGTGATTCGGATGGTG
GAGATGCAAAAGGACCCCATGGAGCCACCTCGATTCAAAATTAATAAGAAAATTCCACAT
GGACCTCCATCTCCTCCAGCGACAGTAATGCATTCCCCCAGCAGAAAGATGACTGTCAAG
GAACAGCAAGAGTGGAGAATCCCCCCATGTATTTCTAACTGGAAGAATGCCAAGGGTTAT
ACCATCCCACTAGACAAGCGTTTGGCTGCTGATGGCCGAGGGCTGCAGACTGTTCATATC
AATGAGAACTTTGCTAAGCTTGCGGAAGCCTTGTACATTGCTGACAGAAAGGCTCGTGAA
GCTGTGGAGATGAGGGCCCAGGTGGAGCGCAAGATGGCACAAAAGGAGAAGGAGAAAAAA
GAGGAAAAACTTAGGGAATTGGCACAGATTGCAAGAGAACGCAGAGCTGGGATCAAGTCC
CATACAGAAAAAGAGGATGGAGAAGCCAAAGAGAGGGATGATATTCGACATGAGAGACGC
AAGGAACGGCAACATGAACGGAACATATCTAGGGCAGCCCCAGACAAGAGGTCCAAACTC
CAAAGGAATGAAGAAAGAGACATTAGTGAACAGATTGCGCTTGGAATTCCTAATCAGAGG
ACATCCAGTGAAATCCAGTATGACCAACGACTTTTCAACCAAAGCAGAGGTATGGATAGT
GGCTTTGCTGGTGGAGAGGATGAGGTATATAACGTGTATGACCAGCCATGGTTAGGCAAC
AAGAAACTGGCGCAGAACATCTACCGTCCCAGTAAGAATACGGACAACGATGTTTATGGT
GATGACCTGGACACATTAGTGAAAACCAACAGGTTTGTTCCTGACAAGGACTTTTCTGGT
GCAGATCGCAGGCAGCGCCATGAGGGGCCTGTGCAATTTGAAGAGGATCCTTTTGGTTTG
GACAAGTTTTTGGAGGAGGCTAAACAGCATGGAGGATCTAAGCGTCCCTCTGATAGTGGT
CGGGCTAAAGATCATGACCATGATATGAAGAAGCGCAGGAAGGAGTGA

Protein sequence predicted based on nucleotide sequence

MALASFLPAPTQLSQDQLEAEEKLRAQKSRQTALVSSRREPPPYGHRKGWVPRSLEDFGD
GGAFPEIHVAQYPLDMGRKKKMSNALAVQVDAEGKIKYDAIARQGQSKDKVLFSKYTDLV
PKEVMDEDDPELQRPDEEAIKELTEKTRQALDKSVSQKIAAAMPVRAADKLAPAQYIRYT
PSQQGVAFNSGAKQRVIRMVEMQKDPMEPPRFKINKKIPHGPPSPPATVMHSPSRKMTVK
EQQEWRIPPCISNWKNAKGYTIPLDKRLAADGRGLQTVHINENFAKLAEALYIADRKARE
AVEMRAQVERKMAQKEKEKKEEKLRELAQIARERRAGIKSHTEKEDGEAKERDDIRHERR
KERQHERNISRAAPDKRSKLQRNEERDISEQIALGIPNQRTSSEIQYDQRLFNQSRGMDS
GFAGGEDEVYNVYDQPWLGNKKLAQNIYRPSKNTDNDVYGDDLDTLVKTNRFVPDKDFSG
ADRRQRHEGPVQFEEDPFGLDKFLEEAKQHGGSKRPSDSGRAKDHDHDMKKRRKE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable