mybpc3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACTGTGGAACTCGAACCTC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCCTGAGCCTGTCAAGAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00711845
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 3852nts
Open reading frame: 1 to 3852

ATGCCTGAGCCTGTCAAGAAATCAGCGTTTACCAAAAAACCAAAGTCTGAGGAGGTCTCA
GTGGGAGGAACGGTTACATTTGTGGCAGAGACAGAAAAACCTGGTTTGAAGGTGAAGTGG
CAGCGGAACAATGTTGACATCACTGCAAATGAGAGATTTGCCATAAAGTCTGAGGGCAAA
CAACACTCACTGACCATCAGCAATGCCATCACAGAGGATGAAGTAGTCTATGCTGTCATT
GCAGGAACCTCTAAGGTCAAGTTTGAGTTAAAAGTCAAAGAACAAGAAAAAACTGAGGAA
TCTCCTGCCCCAGTTTCAAATCCAGATCCAGCTCCAGCTCCAGCTGCTCCTGAAGCTTCT
GAAGCACCTGCCCCACCAGCCTCTGATGGCTCAGCTCCACCTCCCACTGAAGCTGCTGAG
GCTAAACCCCCAGAGTCTTCTGAAGCTACTGCTACTCAACCAGAACCCCCTAAGGATGAA
ATCGGACTATTCCTGGAAAGACCACAGGATGGGGAAATCACTGTTGGCACAAACATCAAG
TTTACAGCCCGAATTGCCGGAGCCAATTTGATTAAGAAGCCCGTAGTCAAATGGTTCAAA
GGCAAATGGATGGACCTGACCAGCAAAGTTGGCAAACATTTGCAGCTCCATGAGAGTTAT
GACAGGAACACCAAGATTTACACCTTTGAGATACAGATAATTGGGGCAAAAACCACCTAT
GCCGGGGGTTACCGTTGTGAGGTTTCCTCAAAAGACAAGTTTGACAGCTGCAATTTCAAC
CTAGCCGTTCATGAGGCCGCTTCATCAGGAGAGGTAGACATTCGCGCAGCATTCAGGCGC
ACGAGCCTTGTAGGAGCAGCAAAAAGAAGAGTATCCATTGCCTTCAGCGGAGATGGAACT
GAGGAAGCAGGGGAACTGGATTTCAGTGCCTTGCTCAAAAAGAGAGACAGTTTCCTCCGC
TCCGTGAATCGTAATGAGCCAAAGCAGGGCACTGAGCCCGATGTGGATGTGTGGGAAATC
CTCAAGAAAGCACCCCCCTCTGAATATGAGAAGATTGCCTTCCAGTACGGTATCACTGAC
TTGAGGGGCCTCTTGAAACGGCTCAAGAAGATGAAGAAGGAGGAGAAGAAGAGTGAAGCT
TTCCTGAAGAAGATGGATCCTGCATACCAAATAGACAAAGGTCAGAAGATGAAGTTGGTC
GTAGAAGTGGCCAACCCAGATGCAGAGGTCAAATGGCTGAAAAATGGCCAAGAGATCAGA
GTCAGTGGAAGCAAGTACATATTTGAGAGTATTGGGAACAAGAGGATTCTCACCATCAAT
AACTGCTCCCTGGCAGATGACGCAGCCTATACGTGTGTGATTGGAGAGGAGAAGTGCTTC
ACTGAGCTGTTTGTGAGAGAACCTCCCATTCAGATCCTTCATCCCTTGGAAGATCAGATG
GTGATGGTGGGAGAGAGAGTGGAGTTTATGTGTGAAGTGTCAGAGGAAGGAGCACAAGTG
AAATGGGAGAAAGATGGTGTGGAACTGACCAGAGAGGAGACCTTCAAGTATCGGTTTAAA
AAAGATGGCAAAAAGCATTATCTGATCATCAATGAGACAACGGTGGAAGATGGGGGCCAC
TACAAAGTCAAAACCAATGGAGGAGATTCATTGGCCGAGCTGATGGTACAAGAGAAACAG
TTGGAAGTTCTACAAGATGTTGCTGATTTGACTGTTCGGGCCAAGGAACAAGCTGTCTTC
AAGTGTGAGGTCTCGGACGAGACAGTGACAGGAATCTGGGTGAAGAATGGAAAGGAAGTC
ATTGCGAACAACAGAATCAAGATCACCCATATCGGAAGGGTACACAAGCTGACCATCGAT
GATGTTGTACCACAGGATGAAGGAGACTACTCCTTTATCCCTAATGGATTTGCTTTCAGC
CTTTCAGCAAAACTACACTTCATGGAGGTGAAGATTGACTTTGTTCCGAGACAAGAGCCC
CCCAAGATCAAACTGGACTGCATGTCTCAATGTGCTGATACCATCATTGTCGTTGCTGGC
AATAAATTACGTCTTGACGTCCCCATATCTGGGGATCCAGCCCCAACTGTTGTGTGGATG
AAGGGGGATAAGGTAATTGAGCAGATCAAGGTCAGGACTGAAACTGAGTCTCAACACGAC
TTATACAGAGACGTGGATCTAATTTCAGAGGTTGATGGCAGAGTCCACGTGGAGACACAC
CCAGACCATTGTGTGTTCACCATTGAGGGGGCAGAGAAATCAGATGAAGGGGCCTACACC
GTAGTGGTGAAGAATGAAGCTGGAGAGGACAAAGCTAACATCAATGTAAAGGTTATTGAG
GTCCCAGATCCACCTGAGGCCCCCAGGATATCAAACATTGGTGAAGATTTTTGCACAGTA
CAATGGGATCCACCCAAGTATGATGGAGGCCAACCAATTTTGGGCTACATCTTGGAGAGG
AAGAAAAAGAAGAGTTACCGCTGGATGCGGCTGAATTACGACCTGGTTAAGGAATTAACC
TACGAATCTAAGAGAATGATTGAGGGAGTGGTATATGAGATGCGCATATATGCTGTAAAT
TCCATTGGCATGTCCATCCCCAGTCAACATTCACAGCCCTTCATGCCTATTGCTCCCACC
AGCGAGCCCACCATGCTGGTGGTGGATGACGTCAGTGACACCAGCATAAGCTTAAAGTGG
AGACCCCCAGAGCGCATTGGCGCAGGAGGCCTCGATGGCTACACTGTGGAATACTGCAAG
GAAGGGTCCACAGAGTGGGTCCCAGCACTGCTAGGCCTCACAGAGAGAACTTCAGTCATG
ATCCGTGACCTGCCGACCGGAGAGAGACTCGCCTTCAGAGTGCGAGCTATCAATCTGGCA
GGACCCAGCGAGCCATGTACCATGAAAGAACCTGTAACCATCCGTGAAATCATGCAACGT
CCAAAGATCTGGCTACCACGTTATCTGAGACAGAAACTGATCAAGAAAGTTGGGGAAACA
GTGAACATTGTCATTCCCTTCCAGGGTAAGCCACGCCCAGTGGTGACCTGGCTGAAAGAT
GGACAGCCTGTTGATCCAAAACAAATTGGAATCCGCAACAGCGAAGCTGACAGCATTTTG
TTCATACGAAAAGCAGAGCGTGATCATTCTGGGGAATATAAGGTTCAAATCCAGATCGAG
AACTGCGAGGACTCAGCCACCATCCACATCCAAATCGTGGATAAGCCTAGTGCCCCTCAG
AACTTAAAGCTTGTAGAAATCTGGGGATTCAATGTAGCACTGGAATGGGTTCCTCCCAAA
GATGATGGAAACACAGAAATTACCGGATACACGGTCCAGAAAGCAGATAAAAAGACCATG
GAATGGTTCACAGTCTTTGAGCACTATCGGCGAACACATTGCGTTGTGTCAGATCTGATC
ATGGGCAACGAGTATTTCTTCCGCGTGTTCAGTGAGAACATGTGTGGCCTGAGCGAGAAT
CCTTGCACGTCCAAGAACAGCGCCTATATCCAAAAAACCGGAACTGCCTACAAGCCTCCC
AGTTACAAAGATCATGAATTTGAGGAAGCCCCTAAGTTTACTCACCCATTGAATGACCGT
TCTGTTATCGCTGGTTACAATGCCACCCTCAGCTGTGCTCTACGAGGAATACCCAAGCCC
AAGATTACATGGTATAAGAATAAGATGGACCTGTCTATGGAGGCTCGGTATCGCTCATTC
AGTAAGCAGGGGGTGCTTACATTGGAATTGAGGAAGCCAAGCCCATTCGATGGCGGTGTC
TACACATGCAAAGCAGTCAATGAGCACGGGGTGGCAGAAACTGAATGCCGACTGGAGGTT
CGAGTTCCACAGTAA

Protein sequence predicted based on nucleotide sequence

MPEPVKKSAFTKKPKSEEVSVGGTVTFVAETEKPGLKVKWQRNNVDITANERFAIKSEGK
QHSLTISNAITEDEVVYAVIAGTSKVKFELKVKEQEKTEESPAPVSNPDPAPAPAAPEAS
EAPAPPASDGSAPPPTEAAEAKPPESSEATATQPEPPKDEIGLFLERPQDGEITVGTNIK
FTARIAGANLIKKPVVKWFKGKWMDLTSKVGKHLQLHESYDRNTKIYTFEIQIIGAKTTY
AGGYRCEVSSKDKFDSCNFNLAVHEAASSGEVDIRAAFRRTSLVGAAKRRVSIAFSGDGT
EEAGELDFSALLKKRDSFLRSVNRNEPKQGTEPDVDVWEILKKAPPSEYEKIAFQYGITD
LRGLLKRLKKMKKEEKKSEAFLKKMDPAYQIDKGQKMKLVVEVANPDAEVKWLKNGQEIR
VSGSKYIFESIGNKRILTINNCSLADDAAYTCVIGEEKCFTELFVREPPIQILHPLEDQM
VMVGERVEFMCEVSEEGAQVKWEKDGVELTREETFKYRFKKDGKKHYLIINETTVEDGGH
YKVKTNGGDSLAELMVQEKQLEVLQDVADLTVRAKEQAVFKCEVSDETVTGIWVKNGKEV
IANNRIKITHIGRVHKLTIDDVVPQDEGDYSFIPNGFAFSLSAKLHFMEVKIDFVPRQEP
PKIKLDCMSQCADTIIVVAGNKLRLDVPISGDPAPTVVWMKGDKVIEQIKVRTETESQHD
LYRDVDLISEVDGRVHVETHPDHCVFTIEGAEKSDEGAYTVVVKNEAGEDKANINVKVIE
VPDPPEAPRISNIGEDFCTVQWDPPKYDGGQPILGYILERKKKKSYRWMRLNYDLVKELT
YESKRMIEGVVYEMRIYAVNSIGMSIPSQHSQPFMPIAPTSEPTMLVVDDVSDTSISLKW
RPPERIGAGGLDGYTVEYCKEGSTEWVPALLGLTERTSVMIRDLPTGERLAFRVRAINLA
GPSEPCTMKEPVTIREIMQRPKIWLPRYLRQKLIKKVGETVNIVIPFQGKPRPVVTWLKD
GQPVDPKQIGIRNSEADSILFIRKAERDHSGEYKVQIQIENCEDSATIHIQIVDKPSAPQ
NLKLVEIWGFNVALEWVPPKDDGNTEITGYTVQKADKKTMEWFTVFEHYRRTHCVVSDLI
MGNEYFFRVFSENMCGLSENPCTSKNSAYIQKTGTAYKPPSYKDHEFEEAPKFTHPLNDR
SVIAGYNATLSCALRGIPKPKITWYKNKMDLSMEARYRSFSKQGVLTLELRKPSPFDGGV
YTCKAVNEHGVAETECRLEVRVPQ

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable