ctnnb1-a (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACAAGTCAGTGTCAAACCAG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCAACTCAAGCAGATCTAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00712599
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2343nts
Open reading frame: 1 to 2343

ATGGCAACTCAAGCAGATCTAATGGAGCTGGACATGGCCATGGAGCCAGACCGAAAAGCA
GCAGTCAGTCACTGGCAGCAACAGTCTTACCTGGATTCTGGTATTCATTCTGGAGCAACC
ACCACAGCACCATCTTTGAGTGGCAAAGGAAATCCAGAGGATGATGATGTGGATCCCAAC
CAAGTTTTGTATGAGTGGGAGCAAGGCTTCACTCAGTCTTTCACTCAAGATCAAGTGGCT
GATATTGATGGTCAGTATGCTATGACAAGAGCCCAGCGAGTTCGTGCTGCAATGTTTCCA
GAAACCCTTGATGAAGGCATGCAGATTCCATCCACGCAGTTTGACTCTGCACACCCAACA
AATGTACAACGTTTAACAGAGCCTTCTCAGATGCTCAAACATGCTGTGGTTAACTTGATC
AATTACCAGGATGATGCTGAACTGGCCACTCGAGCAATCCCCGAGCTTACAAAACTACTT
AATGATGAGGACCAGGTTGTAGTTAACAAGGCTGCTGTAATGGTACACCAACTGTCAAAG
AAGGAAGCATCACGCCATGCTATAATGCGTTCACCACAGATGGTGTCTGCAATAGTTCGT
ACAATGCAGAACACAAATGATGTAGAAACTGCACGATGCGCTGCAGGGACACTTCACAAC
CTTTCTCACCATCGTGAAGGCTTGTTGGCTATCTTTAAATCTGGAGGCATTCCTGCTCTG
GTTAAGATGCTTGGCTCCCCAGTCGACTCTGTGCTATTCTATGCCATCACAACTCTGCAC
AATCTACTTCTTCACCAAGAAGGCGCAAAGATGGCTGTACGCCTGGCTGGTGGCTTGCAA
AAAATGGTTGCCCTTCTCAATAAAACAAATGTTAAATTCTTGGCTATCACGACAGACTGT
CTTCAAATATTGGCCTATGGAAATCAAGAAAGCAAGTTGATCATTCTTGCAAGTGGGGGA
CCGCAAGCCTTGGTGAATATCATGAGGACTTACAGTTACGAGAAACTTCTGTGGACCACA
AGTCGGGTGCTTAAGGTGTTATCTGTGTGCTCTAGTAACAAGCCTGCCATAGTTGAAGCT
GGTGGAATGCAAGCTTTAGGACTCCATCTCACAGACTCAAGCCAACGTCTGGTTCAGAAT
TGTCTTTGGACACTAAGAAACCTTTCAGATGCAGCAACTAAACAGGAGGGAATGGAAGGT
CTTCTTGGAACCCTGGTTCAGCTTCTTGGATCAGATGACATAAATGTTGTAACTTGTGCT
GCTGGTATATTGTCCAACCTCACATGCAACAACTACAAAAATAAAATGATGGTTTGTCAA
GTTGGAGGCATTGAAGCACTTGTGCGCACTGTTCTGCGTGCTGGGGATAGAGAAGACATC
ACTGAACCTGCAATTTGTGCATTGCGACATCTTACAAGTAGGCATCAGGAAGCCGAGATG
GCTCAAAATGCAGTCCGTCTCCATTATGGACTTCCAGTGGTGGTGAAGCTCTTGCACCCT
CCTTCACATTGGCCTTTGATAAAGGCTACCGTTGGCTTGATCCGCAATCTTGCCCTTTGT
CCAGCCAATCATGCCCCACTGCGTGAACAGGGCGCTATTCCTCGACTTGTCCAGTTGCTT
GTTCGTGCGCACCAGGACACACAGAGGCGTACTTCAATGGGTGGAACACAACAGCAATTT
GTGGAGGGTGTCCGTATGGAAGAGATTGTTGAAGGCTGCACAGGTGCCCTTCACATTTTG
GCTCGTGATGTCCACAACCGGATTGTAATCAGAGGTCTTAACACCATTCCATTATTTGTG
CAGTTGCTGTACTCTCCTATTGAAAATATCCAAAGAGTGGCAGCAGGCGTGCTTTGTGAG
CTGGCACAAGACAAAGAGGCAGCTGAGGCTATTGAAGCTGAAGGTGCAACTGCTCCTCTT
ACTGAACTGCTTCACTCTAGAAATGAAGGTGTTGCAACTTATGCAGCTGCTGTTCTCTTC
CGTATGTCTGAGGACAAACCGCAGGACTACAAGAAACGTCTGTCCGTTGAGTTGACAAGC
TCTCTCTTCAGAACTGAGCCAATGCCATGGAATGAGGCTGCAGACCTTGGTCTTGATATT
GGTGCCCAAGGTGAACCTCTTGGCTACAGACAGGATGATCCAGGCTACAGATCTTTCCAT
GCTCCTGGCTATGGTCAAGATGCAATGGGCATGGACTCCATGATGGATCATGACATGGGA
GGTCATCACCCAGGAGCAGACTATCCAGTTGATGGACTTCCTGATTTGAGTCATGCCCAA
GATCTCATGGATGGGCTTCCTCCAGGTGATAGCAACCAACTGGCCTGGTTTGACACTGAC
TTGTAA

Protein sequence predicted based on nucleotide sequence

MATQADLMELDMAMEPDRKAAVSHWQQQSYLDSGIHSGATTTAPSLSGKGNPEDDDVDPN
QVLYEWEQGFTQSFTQDQVADIDGQYAMTRAQRVRAAMFPETLDEGMQIPSTQFDSAHPT
NVQRLTEPSQMLKHAVVNLINYQDDAELATRAIPELTKLLNDEDQVVVNKAAVMVHQLSK
KEASRHAIMRSPQMVSAIVRTMQNTNDVETARCAAGTLHNLSHHREGLLAIFKSGGIPAL
VKMLGSPVDSVLFYAITTLHNLLLHQEGAKMAVRLAGGLQKMVALLNKTNVKFLAITTDC
LQILAYGNQESKLIILASGGPQALVNIMRTYSYEKLLWTTSRVLKVLSVCSSNKPAIVEA
GGMQALGLHLTDSSQRLVQNCLWTLRNLSDAATKQEGMEGLLGTLVQLLGSDDINVVTCA
AGILSNLTCNNYKNKMMVCQVGGIEALVRTVLRAGDREDITEPAICALRHLTSRHQEAEM
AQNAVRLHYGLPVVVKLLHPPSHWPLIKATVGLIRNLALCPANHAPLREQGAIPRLVQLL
VRAHQDTQRRTSMGGTQQQFVEGVRMEEIVEGCTGALHILARDVHNRIVIRGLNTIPLFV
QLLYSPIENIQRVAAGVLCELAQDKEAAEAIEAEGATAPLTELLHSRNEGVATYAAAVLF
RMSEDKPQDYKKRLSVELTSSLFRTEPMPWNEAADLGLDIGAQGEPLGYRQDDPGYRSFH
APGYGQDAMGMDSMMDHDMGGHHPGADYPVDGLPDLSHAQDLMDGLPPGDSNQLAWFDTD
L

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable