irx3 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  irx3
Gene Name:  iroquois homeobox 3
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGGATGAGGATAAAGCGGA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGTCCTTCCCACAGCTGGGCTA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713060
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1344nts
Open reading frame: 1 to 1344

ATGTCCTTCCCACAGCTGGGCTACCAGTACATCAGACCTCTGTACCCCTCGGACAGACAG
AGCGTGGGGGTAACCAGGAGCGGAACTGAGCTGTCTCCGGCAGGGACTCTTTCTAATGTA
CTTTCCTCCGTGTATGGAGCACCCTACGCTGCAGCCGCCGCAGCACAAGCCTATGGAGCC
TTCCTGCCCTACAGCGCGGAGCTGCCCATCTTCCCCCAGCTGGGTTCACAGTATGACATG
AAGGACAGTCCTGGGGTCCAGCATGCCGCCTTCTCCCACCCTCACCCAGCCTTTTATCCC
TACGGACAATACCAATTTGGAGATCCGTCAAGGCCCAAAAATGCTACCAGAGAGAGCACA
AGCACCCTTAAGGCCTGGCTCAATGAGCACAGAAAGAACCCCTATCCGACCAAGGGCGAG
AAAATCATGCTTGCTATCATCACTAAAATGACCCTCACGCAGGTGTCCACCTGGTTTGCT
AACGCAAGGAGGAGACTTAAAAAGGAGAACAAAATGACTTGGGCCCCTAGGAGCCGAACA
GACGAGGAAGGTAACGCCTATGGCAGCGACCACGAAGAAGACAAGCACGAGGACGAGGAG
GAGATTGACTTGGAGAACATCGACACTGAGGACATTGAGAGTAAAGAAGATTTGGATGAC
CCAGACACTGATATACACTCGGACTCTAAAACTGATACTAGGAGTGACTCGGAAGTCTCG
GATGGGTTTGAAGACCTAAATGTCCCTGAGGACAGACTTCTCAAATCTGTGGTTGGTCAA
AGGCAACTTTTAAATGAGGAGCCTCAGGACAAGTGTGCACTTTCTTCTGATGCTAAAGTT
CCACAACCTGCATGTGAGCAGATCAAACTTAACAGACTCCCCCCTAGTCCTCCTCAGGAG
AACAACATGCCCGTAGCCCATAAACCCAAAATCTGGTCATTGGCAGAGACTGCCACAACC
CCAGATAACCCACGCAATTCTCCCAATACTGGGGGCTCAGTGAACACACAAAACCTGATT
GCACAACACAGACTTATTGCCTCCCCAGGGAGCAGGTTCCAGGGTTGGACTGGGAGAGCT
TTCTCTGCTCAGCAGTTGTCCTTACTGAACTCGGCTCATTTTCTACAAGGACTCGGTGTC
TCTCACACAGCCCCATGTTCTGGCAATGCAAGTTTCCCTAAGGCCGCGGAGTCCAAGTGC
AGCACAAACTCTCTAACAGATCGACCCAGTACAGTGGACATAGAGAAAACAATACCTGTA
TTAAATACAGCTTTCCAACCAGTGCAACGGAGGTCACAAAATCACCTGGACGCTGCAATG
ATTCTTTCCGCTTTATCCTCATCCTAA

Protein sequence predicted based on nucleotide sequence

MSFPQLGYQYIRPLYPSDRQSVGVTRSGTELSPAGTLSNVLSSVYGAPYAAAAAAQAYGA
FLPYSAELPIFPQLGSQYDMKDSPGVQHAAFSHPHPAFYPYGQYQFGDPSRPKNATREST
STLKAWLNEHRKNPYPTKGEKIMLAIITKMTLTQVSTWFANARRRLKKENKMTWAPRSRT
DEEGNAYGSDHEEDKHEDEEEIDLENIDTEDIESKEDLDDPDTDIHSDSKTDTRSDSEVS
DGFEDLNVPEDRLLKSVVGQRQLLNEEPQDKCALSSDAKVPQPACEQIKLNRLPPSPPQE
NNMPVAHKPKIWSLAETATTPDNPRNSPNTGGSVNTQNLIAQHRLIASPGSRFQGWTGRA
FSAQQLSLLNSAHFLQGLGVSHTAPCSGNASFPKAAESKCSTNSLTDRPSTVDIEKTIPV
LNTAFQPVQRRSQNHLDAAMILSALSSS

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable