rho (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  rho
Gene Name:  rhodopsin
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATGCAGGAGACACCTGGCTGGA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAACGGAACAGAGGGTCCCAA, XGC Collection: IRBG
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713515
Price: 
Login for Pricing
AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1062nts
Open reading frame: 1 to 1062

ATGAACGGAACAGAGGGTCCCAATTTTTATATCCCCATGTCCAACAAAACTGGGGTGGTA
CGAAGCCCATTCGATTACCCTCAGTATTACTTAGCAGAGCCATGGCAATATTCAGCACTG
GCTGCTTACATGTTCCTGCTCATCCTGCTTGGGTTACCAATCAACTTCATGACCTTGTTT
GTTACCATCCAGCACAAGAAACTCAGAACACCCCTAAACTACATCCTGCTGAACCTGGTA
TTTGCCAATCACTTCATGGTCCTGTGTGGGTTCACGGTGACAATGTACACCTCAATGCAC
GGCTACTTCATCTTTGGCCAAACTGGTTGCTACATTGAAGGCTTCTTTGCTACACTTGGT
GGTGAAGTGGCCCTCTGGTCACTGGTAGTATTGGCCGTTGAAAGATATATGGTGGTCTGC
AAGCCCATGGCCAACTTCCGATTCGGGGAGAACCATGCTATCATGGGTGTAGCCTTCACA
TGGATCATGGCTTTGTCTTGTGCTGCTCCTCCTCTATTTGGATGGTCCAGATACATCCCA
GAGGGAATGCAATGCTCATGCGGAGTAGACTACTACACACTGAAGCCTGAGGTCAACAAT
GAATCCTTTGTTATCTACATGTTCGTTGTCCACTTCACCATTCCCCTGATTGTCATCTTC
TTCTGCTATGGCCGCCTGCTCTGCACTGTCAAAGAGGCTGCAGCCCAGCAACAGGAATCT
GCTACCACCCAGAAGGCTGAGAAAGAGGTCACCAGAATGGTTGTTATCATGGTCGTTTTC
TTCCTGATCTGTTGGGTGCCCTATGCCTATGTGGCATTCTACATCTTCACCCACCAGGGC
TCCGACTTTGGCCCAGTCTTCATGACTGTCCCAGCTTTCTTTGCCAAGAGCTCTGCTATC
TACAATCCTGTCATCTACATTGTTTTGAACAAACAGTTCCGTAACTGCTTGATCACCACC
CTGTGCTGTGGAAAGAATCCATTCGGTGATGAAGATGGCTCCTCTGCAGCCACCTCCAAG
ACAGAAGCTTCTTCTGTCTCTTCCAGCCAGGTGTCTCCTGCATAA

Protein sequence predicted based on nucleotide sequence

MNGTEGPNFYIPMSNKTGVVRSPFDYPQYYLAEPWQYSALAAYMFLLILLGLPINFMTLF
VTIQHKKLRTPLNYILLNLVFANHFMVLCGFTVTMYTSMHGYFIFGQTGCYIEGFFATLG
GEVALWSLVVLAVERYMVVCKPMANFRFGENHAIMGVAFTWIMALSCAAPPLFGWSRYIP
EGMQCSCGVDYYTLKPEVNNESFVIYMFVVHFTIPLIVIFFCYGRLLCTVKEAAAQQQES
ATTQKAEKEVTRMVVIMVVFFLICWVPYAYVAFYIFTHQGSDFGPVFMTVPAFFAKSSAI
YNPVIYIVLNKQFRNCLITTLCCGKNPFGDEDGSSAATSKTEASSVSSSQVSPA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable