ubc (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  ubc
Gene Name:  ubiquitin C
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAATCCCACCCCTCAAACGCA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCAGATATTTGTGAAAACTCTC, XGC Collection: IRBG
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713538
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1827nts
Open reading frame: 1 to 1827

ATGCAGATATTTGTGAAAACTCTCACTGGGAAGACCATCACACTAGAAGTTGAGCCAAGT
GATACTATTGAGAATGTGAAGGCTAAAATTCAGGATAAAGAAGGCATTCCTCCAGACCAA
CAAAGGTTGATTTTTGCTGGCAAACAGCTGGAAGATGGTCGAACCCTGTCAGACTATAAC
ATTCAAAAGGAATCCACTCTGCACTTGGTTCTCCGTTTGAGAGGTGGCATGCAGATATTT
GTGAAGACCTTGACAGGGAAGACAATTACTCTTGAGGTAGAGCCTAGTGACACAATTGAA
AATGTCAAGGCCAAAATCCAAGACAAAGAAGGTATTCCCCCTGACCAGCAGAGATTGATT
TTTGCTGGTAAGCAACTGGAAGATGGTCGCACTCTTTCTGACTACAACATTCAAAAAGAA
TCCACCTTGCACTTAGTTCTCCGTCTGAGAGGTGGAATGCAAATATTTGTCAAGACCTTA
ACTGGGAAGACAATTACTCTTGAGGTTGAGCCTAGCGATACTATTGAGAATGTCAAGGCC
AAAATCCAAGACAAGGAAGGCATTCCCCCTGATCAACAAAGATTGATCTTTGCTGGCAAA
CAATTGGAAGATGGCAGAACCCTGTCTGATTACAATATTCAGAAGGAATCCACTCTGCAC
TTGGTTCTCCGTTTGAGAGGTGGGATGCAGATATTTGTGAAAACACTGACAGGGAAGACA
ATTACTCTTGAGGTAGAGCCAAGTGATACTATTGAGAATGTCAAAGCCAAAATCCAAGAC
AAGGAAGGCATTCCCCCTGACCAACAAAGATTGATCTTTGCTGGTAAGCAGCTGGAAGAT
GGGAGAACACTATCTGACTACAATATACAGAAGGAATCTACTCTGCACTTGGTCCTTCGT
CTCAGAGGTGGGATGCAGATATTTGTGAAAACACTGACAGGGAAGACAATTACTCTTGAG
GTAGAGCCAAGTGATACTATTGAGAATGTCAAGGCCAAAATCCAAGACAAGGAAGGCATT
CCCCCTGACCAACAAAGATTGATCTTTGCTGGTAAGCAGCTGGAAGATGGGAGAACACTA
TCTGACTACAATATACAGAAAGAATCAACCCTGCATTTGGTTCTCCGTCTGAGAGGTGGG
ATGCAAATATTTGTCAAGACCTTAACTGGTAAAACCATTACCCTTGAAGTTGAGCCAAGT
GACACAATTGAAAATGTTAAGGCAAAAATTCAGGATAAAGAAGGTATTCCCCCTGACCAG
CAGAGATTGATTTTTGCTGGCAAGCAACTGGAAGATGGCCGAACCCTGTCAGACTATAAC
ATCCAGAAAGAATCCACCTTGCACTTAGTTCTCCGTCTGAGAGGTGGAATGCAAATATTT
GTCAAGACATTAACTGGGAAGACAATTACCCTTGAGGTTGAACCAAGTGACACAATTGAA
AATGTAAAAGCCAAAATCCAAGACAAAGAAGGCATTCCCCCTGACCAGCAGAGATTAATT
TTTGCTGGTAAGCAACTGGAAGATGGCAGAACACTGTCTGACTACAATATTCAGAAGGAA
TCCACTCTGCACTTGGTCCTTCGTCTCAGAGGTGGGATGCAGATATTTGTGAAGACCTTA
ACTGGGAAGACCATTACCCTTGAAGTTGAGCCCAGTGACACAATTGAGAATGTAAAGGCA
AAAATCCAGGATAAGGAAGGAATCCCTCCTGACCAACAGAGATTGATCTTTGCTGGCAAG
CAGCTGGAAGATGGTCGCACTCTCTCTGACTACAACATACAGAAAGAATCCACCCTCCAC
CTGGTCCTGCGTTTGAGGGGTGGGATTTAG

Protein sequence predicted based on nucleotide sequence

MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYN
IQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLI
FAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKA
KIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKT
ITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLR
LRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTL
SDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQ
QRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTLTGKTITLEVEPSDTIE
NVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGGMQIFVKTL
TGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLH
LVLRLRGGI

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable