foxi4.2-a (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  foxi4.2-a
Gene Name:  forkhead box I4, gene 2
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTACCTTCCCTGGTACAGA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGGACTTTATTGTGACAACTTC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713632
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1020nts
Open reading frame: 1 to 1020

ATGGGACTTTATTGTGACAACTTCATGTACCAACAGCACAACCTGCACCCCAGCCACAGG
GCGACCAACTTCAGCATCGGGGACTTCACCCACCAAGCGAACCCTTACTTATGGCTGGGG
GGCCCTGGGGTCAACAACTCACCCAGTTACAGCCCTACCCCTGCCCCTTACATACCCCCT
GCCTTCAGCGCCCCCCAGCGGCAGTTCCTGGCCAACTCTGCAGCCTTTGGTGGAGCCGAT
CTGGGCTGGATGTCGGCGGCCTCCCAGGAGGAGCTTCTGAAGAGGGTGAGACCCCCTTAC
TCGTACTCTGCCCTCATCGCTATGTCCATCCAGAATGCGACCGACAAGAGACTCACCCTG
AGCCAGATCTACCAGTATGTGGCAGAAAACTTCCCTTTCTACAAGAAGTGCAAAGCAGGC
TGGCAGAACTCCATCAGGCACAACCTGTCCCTCAATGACTGCTTCAAGAAGATGCCAAGG
GATGAGAATGATCCAGGAAAAGGAAACTACTGGACTCTGGACTCCAACTGCGAGAAGATG
TTTGACAATGGTAATTTCCGCCGGAAGAGAAAGCCCAAATCTGAGACCAACAACATTAAA
ATCGCTAAGAGGGAAGAGGATCACGTGAGCCCAAAAGGCAAAGAAAGCCCACCCATGATT
ACTCCCTCCTCCCCCAAGGAGCTGTCTCCCACAGGCCATAGCAAGTGCCCATCTCCCCCA
ACTGTCACCTACACCCCCTGTCTAACCAACTTCATTGGCAGCATGACTGCCGTGGACTCA
GCTACAATGAACAGACAGGGCCCCCTGGGGCTTCTCAATGAATTATCTCAGAGGAACCTC
AATGGCTTGAGCAGCTTCATCTCGGGCTCCGCAGTTGATCAGAGCCCTGAGCATCAGGAC
AGTAGCCTGTTCTATAACAGAAGCCCCTACTACAGCTCCTTGCCCACCTCTAACCAGAAG
CAGCCACCGTACCTTCAGCAGCTCCATCCCCAGCAGTCTCCTCTGTACCAGGGAAGGTAC
TGA

Protein sequence predicted based on nucleotide sequence

MGLYCDNFMYQQHNLHPSHRATNFSIGDFTHQANPYLWLGGPGVNNSPSYSPTPAPYIPP
AFSAPQRQFLANSAAFGGADLGWMSAASQEELLKRVRPPYSYSALIAMSIQNATDKRLTL
SQIYQYVAENFPFYKKCKAGWQNSIRHNLSLNDCFKKMPRDENDPGKGNYWTLDSNCEKM
FDNGNFRRKRKPKSETNNIKIAKREEDHVSPKGKESPPMITPSSPKELSPTGHSKCPSPP
TVTYTPCLTNFIGSMTAVDSATMNRQGPLGLLNELSQRNLNGLSSFISGSAVDQSPEHQD
SSLFYNRSPYYSSLPTSNQKQPPYLQQLHPQQSPLYQGRY

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable