Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATCGTGACACAACTGAGCTAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGACTGTTTTCAGGCAAGACAA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00713747
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 4281nts
Open reading frame: 1 to 4281
ATGACTGTTTTCAGGCAAGACAATGTGGAGGATTATTATGATATAGGAGAAGAGCTTGGA
AGTGGTCAGTTTGCCGTGGTGAAAAAATGCCAGGAAAAGAAAACAGGCGTGCATTATGCT
GCAAAATGTATCAAGAAGCGAAGGACCAAATCAAGCCGGCGTGGGGTGACCCGAGAAGAT
ATCGAGAGGGAGGTCAGCATCCTGAAGGAGATCCAGCACCCCAATGTGATCACGTTGCAC
GAAGTGTACGAGAACAAAGCAGATGTCATTCTCATTCTGGAGCTAGTCGCAGGAGGTGAA
CTGTTTGACTTTCTTGCTGAAAAAGAGTCTCTGACAGAAGAAGAAGCTACAGAGTTCATC
AAGCAGATCCTTAATGGTGTAAATTACCTTCACTCCCTGCAGATTGCCCACTTTGATCTC
AAGCCTGAGAACATCATGCTGCTGGATAGAAATGCACCCAAGACTCGTATAAAGATCATA
GACTTTGGCCTGGCTCACAGAATCGACTCTGGAAATGAATTCAAGAACATATTTGGAACT
CCAGAATTTGTTGCTCCTGAAATTGTGAATTATGAGCCTCTTGGTCTTGAGTCTGATATG
TGGAGTATCGGAGTCATAACATACATTCTATTAAGCGGAGCATCTCCATTCCTTGGGGAA
ACAAAACAAGAGACCTTGGCTAATATATCTGCTGTCAGTTATGACTTTGAGGAAGAGTTC
TTCAGTAGTACAAGTGACTTGGCCAAAGATTTTATAAGGCGGCTACTAGTCAAGGACCCA
AAGAAGAGGATGACAATTCAAGACAGTTTGTGCCACCCTTGGATTAAGCCTAAAGATACT
CAGCAAGCACTGAGCAGGAAAGCCTCTGCAGTCAATATGGAGAAGTTTAAGAAGTTTGCT
GCTCGCAGAAAATGGAAACAATCTGTCCGTTTGATATCCCTGTGCCAAAGACTGTCGAGA
TCCTTCTTGTCCAGGAGCAACATGAGTATTGCTAGGAGTGATGACACACTGGATGAGGAG
GACTCTTTTGTAATGAAAGCCATCATTCATGCCATAAATGACGATAACGTCCCTGGTTTG
CAGCATCTTTTTGGGTCCTTGTCAAACTATGATGTGAACCAGCCGAATAAGCATGGCACC
CCACCCTTGTTAATTGCTGCTGGCTGTGGGAATATCCAGATTCTTGAACTGCTAATAAAG
CGCGGCGCTCAGATTGATGTACAAGATAAGGCTGGATCCAATGGAATTTACTGGGCTTCT
CGACATGGTCATGTTGAGACTCTGAAATTTCTCCATCAGAATAACTGTCCTTTAGATATT
AAAGATAAGTCGGGCGAAACAGCACTTCACGTGGCGGCCCGCTATGGGCATTTTGAAGTT
ATTCAATACTTGTGCAGCATTGGATCACATCCAGACATCCAGGATAAGGAGGAAGAAACC
CCTCTGCATTGTGCTGCTTGGCATGGTTACTACCCAGTTGCAAAAGCTTTGTGCCAGGCT
GGCTGCAATGTCAATATTCGCAATCGGGATGGGGAAACCCCCATTCTGACAGCAGCAGCC
AGAGGATACCACGACATTGTGGAGTGCTTGGTTGACCATGGAGGTGACCTTGATGCAACA
GACAAGGATGGGCACATAGCCCTTCACCTTGCAGTGAGACGGTGTCAGATGGAAGTAGTG
AAAATGCTCATCATCCATGGATGCTTTGTAGACTTCCAGGATCGCCATGGTAACACACCT
CTGCATGTTGCCTGCAAAGATGGAAATCTTCCTATTGTAATGGCACTATGTGAGGCAAGA
TGTAATTTGGATATAACTAACAAGTATGGCCGAACACCTTTACATCTGGCAGCAAACAAC
GGTATTGTGGAAGTTGTCCGATATCTCTGTGTCGCAGGAGCAAATGTTGAAGCCTTGACT
TTGGATGGGAAAACAGCAGAGGATCTTGCTAGGTCAGAGCAGCATGAGTCCGTTGTCAGT
CTACTCTCAAGGCTTCGAAAGGATACCTACAGAAGTGTGTTCATACAGCAGCTCAGACCT
TCACCAAACCCCCAACCAAGAATTAAGCTGAAGTTATTTGGACACATGGGATCGGGAAAG
ACCACGCTTGTAGAGTCCCTCAAATGTGGCCTGATCCGAAGTTTCTTCAGAAGACGCCGA
CCTCGTCTTTCATCCACTAACTCTTCCCGGTTTCCTTCTTCTCCACTAACAGCCAAGACA
GTTTCAGTGAGCATCTCGGATCTCTACCCTGGTTGTGAGAATGTGAGTGTAAGAAGCCGG
AGTATGATGTTTGAGCCAGGACTAACCAAAGGGGTTCTTGAAGTATTTGTTTCACCGTCG
CCTCACTGTTGCACAGATGACCAGTCAACCAAGGCTATAGACATCCAGAATGCCAATCTC
AATGGTGTTGGAGATTTCAGTGTGTGGGAATTTTCTGGAAACCCTACGTATTATTGCAGT
TATGATTATTTTGCTGCCAATGATCCCACATCAATTCACATTGTGGTTTTTAGCCTTGAA
GAGCCCTATGAAATTCAACTTAATCAAGTAATATTCTGGCTCAACTTCCTAAAGTCACTT
GTTCCTGTGGAGGAACCAATAGCATATGGTGGGAAGCTAAAGAATCTACTCCACGTTGTA
TTGGTGGCCACACATGCTGATATTGTTAACCTTCCTCGCCCTGCGGGAGGAGAATTTGGT
TATGACAAAAGTCTACCATTGCTAAAAGAAGTAAGAAATAGATTTGGAAATGATCTCCAG
ATTTTGGATAAGCTGTTTGTTCTGGATGCTGGGGCATCAGGGTCCAAGGAAATGAAGCTT
CTGAGGAATCACCTCCAGGAATTAAAAAGCCAGTTGATATCTGATTGTCCACCTATGACA
CCACTGTGCGAGAAGGTGATGTCAACTCTTCCATCCTGGAGAAAGCAGAATGGTCCCAAC
CAACTTCTTTCCCTTCAACAATTTGTTTATGATGTCCAGGATCAACTGAATCCACTAGCA
AGTGAAGAGGATGTTAGAAATCTCGCATTGCAACTGCACAGTATCGGAGAAGTTAATATA
ATGCAGAGTGAGACTGTCCAAGATGTTGTGCTTTTAGATCCACGATGGCTGTGCCACAAT
GTCCTTGGAAAGCTACTGTCTGTAGAAAGCCCAAAAGCCCTACACCATTACAGGGGGAGA
TATACAATGGAAGATATTCAGCGATTGGTCACAGACAGTGATGTTGATGAGCTTGTGCAG
ATCTTGGATGCAATGGACATTTGTGCTCGAGATCTAAGCAGCGGAACTATGGTGGACATC
CCTGCCCTCATTAAAACTGACAACCTCCACAGATCATGGGCTGATGAAGAAGAGGACATA
CTAACTTATGGGGGTGTTAGAATTGTTCCTGTGGAACACATTACTCCTTTTCCATGTGGA
ATTTTTCACAAAGTTCAAGTTAATTTATGCCGATGGGTACATCAACAGAGCGCAGACGGA
GATGCCGACATACGTTTATGGGTGAACGGATGTAAAATAGCCAATCGAGGGGCAGAGGTT
TTGGTGTTGATGGTCAACCACGGCCAAGGAATTGAAGTGCAAGTCAGAGGTCTTGAGACT
GAGAAAATCAAGTGCTGTTTACTTCTTGAATCCATTTGCAGTATCATTGACAACTTATTT
GCAGCAACGTTGCCTGGTCTTTTAACTGTAAAACATTACTTGAGCCCTCAGCAGCTGAGA
GAACATCATGAGCCCCTGATGGTTTACCAACCTAGAGATTTTTTTCGGGCCCAAATACAA
AAGGAGACTTCACTGGCTAACACCATGGCTGGGTACAAAGAGAGCTTTAGCAGCATTCTT
TGTTTTGGGTGTCTAGATGTTTACTCTCAAGGAAACCTGGGTCTGGATCACCTTGTGTCT
GATCTGAATATTCTTGTTAGAAGAAAACTCAGCCGACTTTTAGACCCACCAGACCCCATG
GGAAAGGATTGGTGTCTACTGGCTATGAACTTGGGCCTGCCTGATTTAGTTGCCAAATAC
AATGCTAACAATTCAGTACAAAAAGACTTTTTGCCAAGTCCAATATGTGCTCTTTTGCAG
GAATGGAGCAATTCTCCTGAAAGTACTGTATCACTCTTAATGTCTAAGCTGAGAGAACTT
GGGCGCAGGGATGCAGCAGATTTTTTACTGAGAGCATCCTCTGTTTTTAAGGTGAATCTT
GATATCCATGGACAAGAAGCCTACGCCGCAAGCTGCAACAGCGGCACATCCTATAATTCC
ATTAGCTCAGTTGTGTCACGATGA
Protein sequence predicted based on nucleotide sequence
MTVFRQDNVEDYYDIGEELGSGQFAVVKKCQEKKTGVHYAAKCIKKRRTKSSRRGVTRED IEREVSILKEIQHPNVITLHEVYENKADVILILELVAGGELFDFLAEKESLTEEEATEFI KQILNGVNYLHSLQIAHFDLKPENIMLLDRNAPKTRIKIIDFGLAHRIDSGNEFKNIFGT PEFVAPEIVNYEPLGLESDMWSIGVITYILLSGASPFLGETKQETLANISAVSYDFEEEF FSSTSDLAKDFIRRLLVKDPKKRMTIQDSLCHPWIKPKDTQQALSRKASAVNMEKFKKFA ARRKWKQSVRLISLCQRLSRSFLSRSNMSIARSDDTLDEEDSFVMKAIIHAINDDNVPGL QHLFGSLSNYDVNQPNKHGTPPLLIAAGCGNIQILELLIKRGAQIDVQDKAGSNGIYWAS RHGHVETLKFLHQNNCPLDIKDKSGETALHVAARYGHFEVIQYLCSIGSHPDIQDKEEET PLHCAAWHGYYPVAKALCQAGCNVNIRNRDGETPILTAAARGYHDIVECLVDHGGDLDAT DKDGHIALHLAVRRCQMEVVKMLIIHGCFVDFQDRHGNTPLHVACKDGNLPIVMALCEAR CNLDITNKYGRTPLHLAANNGIVEVVRYLCVAGANVEALTLDGKTAEDLARSEQHESVVS LLSRLRKDTYRSVFIQQLRPSPNPQPRIKLKLFGHMGSGKTTLVESLKCGLIRSFFRRRR PRLSSTNSSRFPSSPLTAKTVSVSISDLYPGCENVSVRSRSMMFEPGLTKGVLEVFVSPS PHCCTDDQSTKAIDIQNANLNGVGDFSVWEFSGNPTYYCSYDYFAANDPTSIHIVVFSLE EPYEIQLNQVIFWLNFLKSLVPVEEPIAYGGKLKNLLHVVLVATHADIVNLPRPAGGEFG YDKSLPLLKEVRNRFGNDLQILDKLFVLDAGASGSKEMKLLRNHLQELKSQLISDCPPMT PLCEKVMSTLPSWRKQNGPNQLLSLQQFVYDVQDQLNPLASEEDVRNLALQLHSIGEVNI MQSETVQDVVLLDPRWLCHNVLGKLLSVESPKALHHYRGRYTMEDIQRLVTDSDVDELVQ ILDAMDICARDLSSGTMVDIPALIKTDNLHRSWADEEEDILTYGGVRIVPVEHITPFPCG IFHKVQVNLCRWVHQQSADGDADIRLWVNGCKIANRGAEVLVLMVNHGQGIEVQVRGLET EKIKCCLLLESICSIIDNLFAATLPGLLTVKHYLSPQQLREHHEPLMVYQPRDFFRAQIQ KETSLANTMAGYKESFSSILCFGCLDVYSQGNLGLDHLVSDLNILVRRKLSRLLDPPDPM GKDWCLLAMNLGLPDLVAKYNANNSVQKDFLPSPICALLQEWSNSPESTVSLLMSKLREL GRRDAADFLLRASSVFKVNLDIHGQEAYAASCNSGTSYNSISSVVSR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
