otx2-a (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  otx2-a
Gene Name:  orthodenticle homeobox 2
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACAAAACCTGGAATTTCCAAGATG, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGATGTCTTATCTCAAGCAACC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714000
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 864nts
Open reading frame: 1 to 864

ATGATGTCTTATCTCAAGCAACCGCCATACGCCGTGAATGGGCTCAGCCTGACCGCCTCC
GGGATGGATTTGTTGCACCAGTCGGTGGGATATCCCGCGACCCCCAGGAAACAGAGGAGG
GAAAGGACCACTTTTACCAGGGCCCAACTGGATATCCTTGAAGCACTTTTTGCCAAAACT
CGTTACCCTGATATCTTCATGAGAGAAGAAGTGGCTCTAAAAATCAACCTACCTGAGTCC
AGAGTCCAGGTCTGGTTCAAAAACCGCAGAGCAAAGTGCCGGCAGCAGCAACAGCAGCAG
CAGAATGGAGGCCAAAACAAAGTGAGACCTTCTAAGAAGAAGACCTCTCCTGTTAGAGAA
GTGAGCTCGGAGAGTGGCACCAGCGGCCAATTCAGCCCACCTAGCAGCACGTCTGTTCCA
GTCATCTCAAGCAGCACAGCCCCTGTGTCCATCTGGAGCCCGGCCTCCGTCTCTCCTCTG
TCCGACCCCCTATCCACATCATCTTCATGTATGCAGAGGTCCTACCCTATGACCTACACG
CAGGCATCAGGGTACAGCCAAGGATATGCAGGCTCAACATCCTACTTTGGGGGTATGGAC
TGTGGATCTTACTTGAGCCCTATGCATCATCAGCTCTCTGGACCTGGAGCTACTTTAAGC
CCAATGGGCACCAATGCAGTGACAAGTCACCTTAACCAGTCCCCAGTTGCCCTCTCCTCA
CAGGCCTATGGAGCTTCTAGCTTGGGTTTTAATTCCACTGACTGCCTGGATTACAAAGAC
CAAACTGCCTCCTGGAAGCTGAACTTCAATGCTGACTGCTTGGATTATAAAGACCAAACC
TCATCTTGGAAATTCCAGGTTTTGTGA

Protein sequence predicted based on nucleotide sequence

MMSYLKQPPYAVNGLSLTASGMDLLHQSVGYPATPRKQRRERTTFTRAQLDILEALFAKT
RYPDIFMREEVALKINLPESRVQVWFKNRRAKCRQQQQQQQNGGQNKVRPSKKKTSPVRE
VSSESGTSGQFSPPSSTSVPVISSSTAPVSIWSPASVSPLSDPLSTSSSCMQRSYPMTYT
QASGYSQGYAGSTSYFGGMDCGSYLSPMHHQLSGPGATLSPMGTNAVTSHLNQSPVALSS
QAYGASSLGFNSTDCLDYKDQTASWKLNFNADCLDYKDQTSSWKFQVL

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable