sftpb (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  sftpb
Gene Name:  surfactant, pulmonary-associated protein B
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAGTGCCACACATGGGTCAA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGAAGGGACACGCCTGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714478
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1179nts
Open reading frame: 1 to 1179

ATGGAAGGGACACGCCTGATCTGGCTACTTACCCTGTGTGCAGCTGCAGTTCTGTCTGGG
AAGGTCCCAGTAAAGGACGACTGCGCCCTGGGCCCGGAGTTCTGGTGTCAGGACCTGATG
ACAGCAGCTCAATGTGGAGCAGTGGATCACTGCAAGCAAACTGCCTGGTTAGGAATAGAT
GTGCTGTGCGTGCAGTGTAAGCAGATTGTGAACATCTTGCTGGACATGGTGAAGGCATCA
CCCATCCAGGACACCATAAAGAAATTCCTGCACAAGCAGTGTTCCCATCTCCCTGTGGTG
CCTCTCATTGCTCAGTGCAACTTGCTGGTGGATCAGTATGAGTCTATGATGGTCACTGTA
TTGGAAAAACAAGTGAACCCTGACGCTCTGTGTTCCACCCTGAGACTTTGCCAATCTGAC
CAAGAAGAATTCTGGAATAATGAGCTGCAACCAGAAATGATCCTTGAAAAAATATTCCCT
TTAATTCAGGAGCATTTGTACAACGCACATGAAAAAGCAACGCAGGGTGATGGTGAGGAT
TTGCCAATACCAAAGCCCATGTGCTGGATGTGCAAGTCGTTTATCAGCCAATTAGAGAAA
GTCATCCCAAAGACAGTCATTGCCAAAGCAGCCTCTCAACTGTGCCGGATCCTTCCAGCG
AAGGTAGCAGGTGTCTGCCAGTGTTTGGTGGAGAAATACACAGTTATTTTACTGGATATA
GTGCTGGAGAAGCTGGGGCCACAGCTGCTGTGCAAATTGCTGTTCATGTGTGCCACGGAT
GAGAACTGCGAGGCAGATTTACCAGTGATACCAGTTCTGGATATTGATTTTGCCTGTGAC
ACATGCCTGGCTGTTACCTCTGCCATTAAACCCACCATAAAGCAGAACATGACCCAGGCA
GAGGTAGAGGCTGCAGTTCTGAAAGCCCATGGAGAGCCGGGCATGGCATGGAAAGAGATC
CAGACTTTCCTTAAGAACCATCACACGGAATTGTCTCTCCTCCTGCACAAGCAGTGGGAC
CACAGGATGACGTGCCAGGCACTGGGAGCTTGTCCTGTTCCTGCTAATGCAGCCGCACAG
CATTCTGGGTGTACCGTGGGACCCTCTTATTGGTGTCAAAACCTGGAAACGGCTAAAGAG
TGTGGGGCCGTCAGTCACTGTTTGACCCATGTGTGGCACTAA

Protein sequence predicted based on nucleotide sequence

MEGTRLIWLLTLCAAAVLSGKVPVKDDCALGPEFWCQDLMTAAQCGAVDHCKQTAWLGID
VLCVQCKQIVNILLDMVKASPIQDTIKKFLHKQCSHLPVVPLIAQCNLLVDQYESMMVTV
LEKQVNPDALCSTLRLCQSDQEEFWNNELQPEMILEKIFPLIQEHLYNAHEKATQGDGED
LPIPKPMCWMCKSFISQLEKVIPKTVIAKAASQLCRILPAKVAGVCQCLVEKYTVILLDI
VLEKLGPQLLCKLLFMCATDENCEADLPVIPVLDIDFACDTCLAVTSAIKPTIKQNMTQA
EVEAAVLKAHGEPGMAWKEIQTFLKNHHTELSLLLHKQWDHRMTCQALGACPVPANAAAQ
HSGCTVGPSYWCQNLETAKECGAVSHCLTHVWH

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable