slc5a8 (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAGCCGTAATTCCATTTGTTTTCTC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGTTACACCCGGGAAT, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714695
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 1863nts
Open reading frame: 1 to 1863

ATGGTTACACCCGGGAATATTGGTTCCTTCACAGTTTGGGACTACCTGGTGTTTGCTCTG
ATGCTGCTGATCTCAGCAGTGATTGGAATCTATTATGCTTTTGCTGGGGGTGGACAGAAG
ACCTCTAAAGATTTCCTGATGGGTGGGAGAAGCATGACTGCTGTGCCAGTGGCCCTATCC
CTAACTGCCAGCTTCATGTCTGCAGTAACCGTGCTGGGTACCCCAGCAGAAGTATACCGC
TTTGGAGCCATGTTCATCATATTTGCCTTTTCATATACCATAGTGGTGATAATCAGCTCC
GAAGTCTTCCTGCCGGTGTTCTACAGACTAGGAATCACTAGTACTTACGAGTACCTTGAA
CTCCGCTTTAATAAGTTTGTCCGGCTCCTCGGCACTATACTTTTCATCATACAAACGGTC
TTGTACACTGGAATTGTTATTTATGCCCCTGCTTTGGCTTTAAATCAAGTGACTGGGTTT
GACCTGTGGGGTGCTGTTGTTGCTACAGGGGTGGTTTGTACCTTCTACTGCACAATGGGA
GGCCTGAAAGCTGTGGTTTGGACAGATGTGTTCCAAGTTGGAATCATGGTGGCTGGTTTT
ACTTCTGTCATCATAAGAGCGGTTGTGGTTCAGGGTGGCATTGGTCCTATTTTGAATGAT
TCTTACTACGGAGATCGATTAAATTTTTGGGATTTTGATCCTAACCCTCTGAAAAGACAT
ACCTTTTGGACAATTGTAGTTGGAGGAACGTTTACTTGGACCGGAATCTATGGAGTAAAC
CAAGCTCAAGTTCAGAGATACATTGCTTGTAAAACCAGGTTCCAAGCTAAAATGTCTTTG
TATGTGAATCTTATTGGACTTTGGGCAATCCTGGCTTGTGCTGTATTGAGTGGATTGGCC
ATGTATTCAATATATAAGGACTGTGATCCCTGGACTGCCAAGTTTGTGTCTGCCCCAGAC
CAGCTGATGCCCTACCTGGCTTTGGATATTCTGCGGGATTACCCAGGACTCCCGGGACTC
TTTGTTTCATGTGCTTACAGTGGGACATTAAGTACGGTTTCCTCCAGTATAAACGCTTTG
GCTGCTGTAACAGTGGAAGACCTTATAAAGCCTTACATCAGATCCCTCAGTGAAAAAAAG
ATGTCTTGGATCTCCAAAGGAACAAGTCTTCTATATGGGGCAATATGCATAGGAATGGCT
GGTATAGCATCTCTGATGGGTGGCTTGTTACAGGCAGCTCTCTCTATTTTTGGCATGGTT
GGGGGTCCTTTGCTGGGCCTGTTTTCGCTGGGGATCCTCTTCCCGTTTGTAAATTCACTA
GGTGCTGTAATTGGTCTCCTGTCTGGCTTTGCAATTTCTCTGTGGGTTGGCATTGGATCC
CAGATTTATGCCCCCTCTCCATCCAGCAGCTTACCCAAACCTCTTTCACTAGAGGGCTGC
AATTTCACAAGCATTGAAAGCAACTGGACTTCCACTGTGATGCCCATGATGACAACCTTA
ATTCCTGAAACACAAGTCAGCAGCAGGCCAGAACTTGCTGATAGCTGGTACTCGCTGTCA
TATCTGTATTTCAGTACTATTGGGACAATAGTTGCAGTTTTAGTTGGTGTGATAGTCAGC
TTGCTTTCAGGAGGGTTAAAGCAAAATGTAAACCGTGAATTCCTCTTAACATCTGAAGAC
TTTTCCTACTTAAATGTTCTGTTCTCTCCTTGCAAGGAGAAAGGGGAAGAAAAAGTTGAA
GTGTTGAACTGGAAGGCACGCAGAACAGATAATGATATGGAGCAAGGCACAGATAACCCC
GCATTCAATAATATGGAAATGACCTCTACGGAAAAGGGAGAGAAAACAAATGGAATTACG
GCTTAA

Protein sequence predicted based on nucleotide sequence

MVTPGNIGSFTVWDYLVFALMLLISAVIGIYYAFAGGGQKTSKDFLMGGRSMTAVPVALS
LTASFMSAVTVLGTPAEVYRFGAMFIIFAFSYTIVVIISSEVFLPVFYRLGITSTYEYLE
LRFNKFVRLLGTILFIIQTVLYTGIVIYAPALALNQVTGFDLWGAVVATGVVCTFYCTMG
GLKAVVWTDVFQVGIMVAGFTSVIIRAVVVQGGIGPILNDSYYGDRLNFWDFDPNPLKRH
TFWTIVVGGTFTWTGIYGVNQAQVQRYIACKTRFQAKMSLYVNLIGLWAILACAVLSGLA
MYSIYKDCDPWTAKFVSAPDQLMPYLALDILRDYPGLPGLFVSCAYSGTLSTVSSSINAL
AAVTVEDLIKPYIRSLSEKKMSWISKGTSLLYGAICIGMAGIASLMGGLLQAALSIFGMV
GGPLLGLFSLGILFPFVNSLGAVIGLLSGFAISLWVGIGSQIYAPSPSSSLPKPLSLEGC
NFTSIESNWTSTVMPMMTTLIPETQVSSRPELADSWYSLSYLYFSTIGTIVAVLVGVIVS
LLSGGLKQNVNREFLLTSEDFSYLNVLFSPCKEKGEEKVEVLNWKARRTDNDMEQGTDNP
AFNNMEMTSTEKGEKTNGITA

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable