kirrel2 (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  kirrel2
Gene Name:  kin of IRRE like 2
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAACACATGGGTCTGCATCCGTT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGAAGAATCTCTGGCTGTTAC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714765
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2325nts
Open reading frame: 1 to 2325

ATGAAGAATCTCTGGCTGTTACTTTGTATCCATGTGGGGCTCCTGCTGGGCAAAAGTTTT
GGGGCCCATTTCTCTCAGCAACCCTCAGACAAGGTCATAGTTGCTGGCAAATCGGTGACT
CTTCCCTGCGTAGTGATTGGCTACAGAGGCGTGGTCCAATGGACGATGGACGGGCTGGCA
CTGGGAGCAGAAAGAGACCTTCCAGGTTGGTCCCGTTATTCTGTTGTTGGAGACCCTTCT
TTAGGAGAACATAACCTGCACATTGAAGGGGTTGAGCTTAGTGATGATGCCATCTACGAG
TGCCAAGCAACACAGGCAGCACTGAGGTCACAGCGGGCACGTCTCACGGTGCTACTTCCC
CCAGGAGACCCAATGATACCAGGATCCCCAGTCGCCAATGTCCTGCTTGATGTACCCTAC
AATCTCACCTGTCTTGCTTCCGTCGCTAAGCCTGCTGCAGAGATTACCTGGTTCCGTGAT
GGAAAGAAGCTGGATTGGGCCATCTACACCAAGGAACTGCTGTCCGATGGCAAGAGTGAG
GGTTCGGCGAGCAGTCTGTTAATCACCCCTAGTCTGGCGGATACAGGATCCAGTTACACC
TGCCGAGTGAGGAATGAGGCCCTGCCAGAGGGCAGAGAGAAGTCTGTGACATTGAGTGTG
CAGTATCCCCCCAAAGTAACGCTGTCGGTAGAACCCCCTACTATCACCGAGGGCGGCTCC
GTCACATTTTTGTGCAGTGCAGTTTCCAACCCAGAAGTAACTGGGTACAGGTGGGCAAAG
GGGGGTGTCCCTCTGGCCGTGTCAGGAGACAAATACACTGTGGAGGTGGATCACACTTTC
TTCACCGCTCCAGTCTCGTGCGAGGTGTCCAACAGCGTGGGAGCCAGTAACGTCAGCACG
GCCGTCAATGTGCTATTTGGGCCACGCCTTCTCACTGAGCCACGCCCTATGACAGTGGAC
ATTGGCGATGACGCCTCTTTCTTTTGTGGCTGGACTGGAAATCCAACGCCTACGCAATTT
TGGAGCAAGAAAGGAACGGGAGAGGTTCTCAGCAATGGAAACACACTGTTACTGACAAAA
GTAACCCGCGAAGATGCCGGAGTATATGTCTGTAAAGCCATTGTGCCACGCATGGGGACT
ACAGAGAAAGAAGTGACCCTCATGGTGAGAGGGCCACCCATCATCACTAGTGAAGCTCAC
TATGAGAGCATACTGGGAGAAAAGGCACGGATGGAGTGTGTCATTGAGTCCACCCCTACC
CCGGACAGAATTGTCTGGGCGTGGGATAAGCAAAGCCTGGAAGAAGGTTCATGGGGCCGT
TTCTCAGTGGAGACACAAGTCACTGAAGTGGGGGCTGTCTCCTTCCTGGTCATTGATGGA
ACGGAGAGGTCGGATTTCCACACTGAGTTCAACTGCAGTGCTATCAATCAGTATGGGGAG
GATTCAGTCATCGTGTCTCTGCGGCAGCAAGCTACTCTTCCTCTGACATTCTTGCTGATT
GCCGTTGGCTTGGGGACCCTGTGCATCTTTGTACTCGTCATCACCTCCATCCTATGTTGC
CGACGACCAAAAAAAGCCGTGAAGGACACGCAGATTCTAGCCGTAGACATGTCGGGGAGC
GAGCCCAGCGAGAGGCACCCCAGTGACTCTGAGGAGGATCTCAAGGAGCCATTGCACACA
GACACGGAATCCCAGAGAACATCCCAGACGGAGCACAGCGACATCCCAGACGAACCCCAG
GACCCAACCAACGGTTATTATAAAGTCCGAGCTCATGAAGACACCAGGCCTACAACCATA
ATGTACCCGGAGTTCTCCTCTCCGCCACGCCCCCTCTACTCCACCACTTCCTCTCTTTCG
CCGTTGTGCCCCACCCCTTCTGGGCCCCCTAAAATCTACGATTATGCTCATCGGTATGCC
ACCACTCCCAGATCTGGAGGTGAGCGCCATCACATGCCACAGGGGTCATCATCTCATTTG
CCGCAAGGCCCCACGCATTTCCCACCTGGCCCAAGTCATGTGTCTCAAGGTCCCACTTAT
TTGCCACAAGGCCCAACTCATTTACCACAAGGCCCAACTCATTTACCGCAAGGCCCAACT
CATTTACCGCAAGGCCCAACTCATTTACAGCAAGGACTCACTCACCTACCACCGGGCCCC
AGTCAGCACCCAGCACCTTACGCCAGGGCTTTCTGTAGCTACGTCCGCCCGGACAGGTTC
GACTCAATGGACCAAGGCCCCACTCTGTCTCGGCTCTCCTATGCCTCCCTAACCAGCCAC
AGTGATTTTGGCCGGCCTTCACAGCAACGGATGCAGACCCATGTGTGA

Protein sequence predicted based on nucleotide sequence

MKNLWLLLCIHVGLLLGKSFGAHFSQQPSDKVIVAGKSVTLPCVVIGYRGVVQWTMDGLA
LGAERDLPGWSRYSVVGDPSLGEHNLHIEGVELSDDAIYECQATQAALRSQRARLTVLLP
PGDPMIPGSPVANVLLDVPYNLTCLASVAKPAAEITWFRDGKKLDWAIYTKELLSDGKSE
GSASSLLITPSLADTGSSYTCRVRNEALPEGREKSVTLSVQYPPKVTLSVEPPTITEGGS
VTFLCSAVSNPEVTGYRWAKGGVPLAVSGDKYTVEVDHTFFTAPVSCEVSNSVGASNVST
AVNVLFGPRLLTEPRPMTVDIGDDASFFCGWTGNPTPTQFWSKKGTGEVLSNGNTLLLTK
VTREDAGVYVCKAIVPRMGTTEKEVTLMVRGPPIITSEAHYESILGEKARMECVIESTPT
PDRIVWAWDKQSLEEGSWGRFSVETQVTEVGAVSFLVIDGTERSDFHTEFNCSAINQYGE
DSVIVSLRQQATLPLTFLLIAVGLGTLCIFVLVITSILCCRRPKKAVKDTQILAVDMSGS
EPSERHPSDSEEDLKEPLHTDTESQRTSQTEHSDIPDEPQDPTNGYYKVRAHEDTRPTTI
MYPEFSSPPRPLYSTTSSLSPLCPTPSGPPKIYDYAHRYATTPRSGGERHHMPQGSSSHL
PQGPTHFPPGPSHVSQGPTYLPQGPTHLPQGPTHLPQGPTHLPQGPTHLQQGLTHLPPGP
SQHPAPYARAFCSYVRPDRFDSMDQGPTLSRLSYASLTSHSDFGRPSQQRMQTHV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable