gapdh (Xenopus laevis) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol:  gapdh
Gene Name:  glyceraldehyde-3-phosphate dehydrogenase
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAATTCCTTAGATGCCATGTGAC, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGTGAAGGTTGGAATTAACGGA, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00714906
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 999nts
Open reading frame: 1 to 999

ATGGTGAAGGTTGGAATTAACGGATTTGGCCGTATTGGGCGCCTGGTGACCCGCGCTGCC
TTTGATAGCGGCAAAGTTCAAGTCACTGCTATCAATGACCCCTTCATCGACTTGGACTAC
ATGGTGTACATGTTCAAGTATGACTCCACCCACGGCCGCTTTAAGGGAACCGTTAAGGCT
GAGAATGGCAAGCTGATCATCAATGACCAAGTCATCACCGTCTTCCAGGAGCGTGACCCC
TCCAACATTAAGTGGGGAGATGCTGGTGCCGTGTATGTGGTGGAATCTACTGGAGTCTTC
ACAACCACAGAGAAGGCCTCTCTGCACTTGAAGGGAGGTGCCAAGCGTGTCGTTATCTCC
GCCCCCTCAGCAGATGCCCCCATGTTTGTAGTTGGCGTGAACCATGAGAAATATGAGAAC
TCTCTTAAAGTTGTTAGCAATGCATCCTGCACTACAAACTGTCTGGCTCCTCTCGCAAAG
GTCATCAACGACAACTTTGGCATTGTTGAGGGACTCATGACAACTGTCCATGCTTTCACT
GCCACCCAGAAGACTGTGGATGGCCCATCAGGGAAGCTGTGGAGAGATGGCAGAGGTGCA
GGTCAGAACATTATTCCCGCCTCAACTGGTGCAGCAAAGGCTGTCGGAAAAGTTATCCCT
GAGCTGAACGGAAAAATAACCGGAATGGCTTTCCGTGTCCCCACCCCAAATGTGTCCGTC
GTGGATCTGACCTGCCGCCTGCAGAAGCCGGCCAAGTACGATGACATCAAGGCCGCCATT
AAGACTGCATCAGAGGGCCCAATGAAGGGAATCCTGGGATACACACAAGACCAGGTTGTC
TCCACTGACTTCAATGGTGACACTCACTCCTCCATCTTTGATGCTGATGCTGGAATTGCC
CTGAATGAAAACTTTGTGAAACTGGTTTCCTGGTATGATAATGAATGCGGCTACAGCCAC
CGTGTTGTGGATCTTATGTGTCACATGGCATCTAAGGAATAA

Protein sequence predicted based on nucleotide sequence

MVKVGINGFGRIGRLVTRAAFDSGKVQVTAINDPFIDLDYMVYMFKYDSTHGRFKGTVKA
ENGKLIINDQVITVFQERDPSNIKWGDAGAVYVVESTGVFTTTEKASLHLKGGAKRVVIS
APSADAPMFVVGVNHEKYENSLKVVSNASCTTNCLAPLAKVINDNFGIVEGLMTTVHAFT
ATQKTVDGPSGKLWRDGRGAGQNIIPASTGAAKAVGKVIPELNGKITGMAFRVPTPNVSV
VDLTCRLQKPAKYDDIKAAIKTASEGPMKGILGYTQDQVVSTDFNGDTHSSIFDADAGIA
LNENFVKLVSWYDNECGYSHRVVDLMCHMASKE

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable