Gene:
Gene Symbol:
Gene Name:
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format:
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAACGGAACTGCAGAGGACTGA, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGCTGACGAAGTTCGAGACC, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00715101
Price:
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AVAILABLE
No restriction
Special MTA: None
Special MTA: None
Insert sequence: 3672nts
Open reading frame: 1 to 3672
ATGCTGACGAAGTTCGAGACCAAGTCCGCCCGAGTGAAAGGGCTGAGCTTTCACCCGAAG
AGGCCGTGGGTCCTCACCAGTTTGCACAATGGTGTCATTCAGCTGTGGGATTACCGCATG
TGCACTCTTATCGACAAGTTTGATGAGCACGACGGTCCCGTCCGTGGCATTGACTTCCAC
AAGCAGCAGCCACTGTTTGTATCTGGGGGAGATGATTATAAAATCAAGGCGTGGAATTAC
AAGCTCCGCCGCTGCCTCTTCACTCTGCTGGGACACCTCGATTATATCCGCACCACCTTC
TTCCATCATGAGTACCCCTGGATCCTGAGTGCATCCGATGATCAGACTATCCGCATCTGG
AACTGGCAGTCTCGCACTTGTGTTTGCGTTCTTACTGGTCACAACCATTACGTCATGTGC
GCTCAGTTCCACCCCTCTGAAGACCTGGTGGTATCTGCCAGCTTGGATCAGACTGTGCGA
GTTTGGGATATTTCTGGCCTGAGGAAGAAGAATCTGTCTCCTGGAGCGGTGGAAGCCGAT
GTCCGGGGGATCTCTGGGGTGGATCTGTTTGGGACTTCGGATGCTGTGGTTAAGCATGTT
CTAGAGGGGCACGATCGTGGAGTTAACTGGGCCGCCTTCCACCCCACCATGCCTTTGATC
GTATCCGGAGCAGATGACCGACAAGTCAAAATCTGGAGGATGAACGAGTCCAAAGCCTGG
GAAGTGGACACATGCCGTGGTCATTACAACAACGTCTCATGCGCCGTCTTTCATCCCCGC
CAAGAGCTGATTCTCAGTAACTCTGAGGACAAAAGCATTAGAGTGTGGGACATGTCCAAA
AGGACTGGAGTCCAGACATTCCGCAGAGACCACGACCGCTTCTGGGTACTCGCCGCCCAC
CCCAATCTTAACCTTTTTGCAGCAGGACATGATGGTGGGATGATCGTGTTCAAGCTGGAA
CGAGAGCGGCCCGCATATGCAGTGCATGGCAATATCTTGTATTATGTGAAGGACAGATTC
CTGCGCCAGTTGGACTTTAACAGCTCCAAGGATGTGGCTGTGATGCAGCTAAGAAGCGGC
TCCAAGTTCCCAGTGTTCAATATGTCGTACAACCCAGCTGAAAATGCCGTCCTGCTTTGC
ACGAGAGCCAGCAACCTGGAAAACAGCACTTATGACCTGTACACCATCCCCAAGGATACC
GACTCCCAGAATCCTGATGCCCCTGAAGGGAAGCGATCCTCTGGTCTGACCGCGGTGTGG
GTGGCGCGGAATAGATTTGCAGTCCTGGATCGCATGCATTCTATTCTCATCAAGAACCTG
AAGAACGAGATCACCAAGAAGGTCCAGGTGCCCAACTGTGATGAGATCTTCTATGCCGGG
ACTGGGAACCTGCTTCTCCGAGACTCTGACTCCATTACTCTCTTTGATGTTCAGCAGAAG
CGCACCCTGGCATCTGTGAAGATCTCCAAAGTGAAGTATGTCATTTGGTCATCAGACATG
TCCCATGTGGCTCTTCTGGCCAAGCATGCTATTATGATCTGCAATAGAAAGCTGGAATCC
CTCTGCAACATCCATGAAAATATCCGCGTCAAGAGTGGCGCTTGGGACGAGAGTGGGGTG
TTCATCTACACTACCAGCAACCACATCAAATACGCACTCACCACCGGAGATCACGGTATT
ATTCGTACACTAGATCTCCCCATTTACATCACACGCGTCAAGGGCAACAATGTATATTGC
CTGGATCGGGAATGCAGACCTCGGGTGCTAACAATTGATCCGACTGAATTCAAGTTCAAG
CTCGCTTTGATCAACCGGAAGTACGATGAGGTGCTGCACATGGTGAGAAACGCTAAACTC
GTGGGTCAGTCCATAATTGCCTACCTCCAAAAGAAGGGCTACCCAGAGGTGGCGCTGCAC
TTTGTGAAGGATGAGAAGACCCGATTCGGCCTGGCTCTGGAATGTGGAAACATTGAGATC
GCCCTTGAAGCAGCCAAGGCTCTGGATGACAAGAATTGCTGGGAGAAGTTGGGAGAGGTG
GCCCTGCTACAGGGGAACCACCAGATTGTGGAGATGTGCTACCAGCGCACCAAGAACTTT
GACAAGCTCTCCTTCCTTTACCTCATCACTGGCAACCTGGAAAAACTGCGCAAGATGATG
AAAATCGCCGAGATCCGCAAAGACATGAGTGGCCAGTACCAGAATGCGCTGTTCCTTGGG
GATGTGGATGAAAGGGTGCGGATACTGAAGAACTGTGGGCAGAAATCTCTGGCCTACCTG
ACAGCCGCCACCCATGGAATCAACGATGAAGAGGAGAGCTTGAGGGAGTCCTTTGACCCA
GAAAAAGAAATAATTCCAGAGGTCGACCCTGATGCTATACTGCTGATGCCCCCACCCCCC
ATCATGCCTCTGGACACTAATTGGCCTTTGTTGACCATGTCTAAGGGCTTCTTTGAAGGA
AGCATTGCAAACAAAGGTAAGGGTGGAGCACTGGCTGCAGATATCGATGTGGATACTGTG
GGAGGTGAAGGCTGGGGAGAAGATGCCGAACTTCAGCTGGATGAAGATGGTTTTGTGGAT
GCTGGGGAAAACTTTGGAGATGAGGTGGCAGGCAAAGGGCATGAAGAGGGTGAAGGATGG
GATGTGGAGGAGGATCTTGACCTTCCCCCTGAGCTGGATGTTCCCTCTGGCCCGTCTGGC
TCAGCTGAAGATGGCTTCTTTGTGCCCCCAACTAAAGGCAGCAGTCCAGCTCAGACATGG
TGTAATAACTCCCAGCTTCCTGTTGATCACATCCTGGCCGGCTCCTTTGAAACTGCAATG
CGGTTGCTCCATGACCAAGTTGGCGTGGTAAACTTTGGCCCATTTAAGCAACACTTCCTC
CATACCTTCTCCCGGGGTCGCAGCACTTACCAGGCCCTGCCAGGTCTCCCTGCCATGTAT
GGCTACCCTCAACGTAATTGGAAGGACGCTGGCTTGAAGAACGGGCTTCCTGCTGTTGGC
CTGAAGCTCAACGACTTGATACAACGCTTACAGCTCTGCTACCAGTTCACTACTTCCGGC
AAGTTTGAGGAGGCTGTGGAGAAGTTCCGGCTGATCCTTCTCAGCGTGCCGCTGCTTGTC
GTGGATAATAAGCAAGAGATTTCAGAGGCCCAGCAGCTGATCACTATTTGTCGGGAATAC
ATTGTTGGTCTGTCCATGGAGATAGATCGCAAGAAACTTCCGAAAGACACTTTGGAGCAA
CAAAAACGCATCTGTGAGATGGCCGCCTATTTCACACACTCCAGCCTGCAGCCCGTGCAC
ATGATTCTGGTACTGAGAACAGCCGTCAACCTCTTCTTCAAACTCAAGAATTTCAAAACC
GCTGCTACTTTTGCCCGGCGACTCCTAGAGCTCGGTCCCAAGCCAGAGGTTGCCCAACAG
ACCCGTAAAATATTGTCCGCTTGTGAGAAGAACCTGACGGACACCTATCAGCTCAACTAT
GACATGCACAACCCCTTCGACATCTGCGCTGCCTCTTACCGGCCCATTTACAGAGGGAAA
CCTGTAGAGAAATGCCCATTGAGTGGAGCCTGCTACAGCCCTGAGTTCAAAGGACAAGTC
TGTAGGGTCACAACAGTGACAGAAATCGGCAAGGATGTGATTGGGTTGAGGATCAGTCCT
CTGCAGTTCCGTTAA
Protein sequence predicted based on nucleotide sequence
MLTKFETKSARVKGLSFHPKRPWVLTSLHNGVIQLWDYRMCTLIDKFDEHDGPVRGIDFH KQQPLFVSGGDDYKIKAWNYKLRRCLFTLLGHLDYIRTTFFHHEYPWILSASDDQTIRIW NWQSRTCVCVLTGHNHYVMCAQFHPSEDLVVSASLDQTVRVWDISGLRKKNLSPGAVEAD VRGISGVDLFGTSDAVVKHVLEGHDRGVNWAAFHPTMPLIVSGADDRQVKIWRMNESKAW EVDTCRGHYNNVSCAVFHPRQELILSNSEDKSIRVWDMSKRTGVQTFRRDHDRFWVLAAH PNLNLFAAGHDGGMIVFKLERERPAYAVHGNILYYVKDRFLRQLDFNSSKDVAVMQLRSG SKFPVFNMSYNPAENAVLLCTRASNLENSTYDLYTIPKDTDSQNPDAPEGKRSSGLTAVW VARNRFAVLDRMHSILIKNLKNEITKKVQVPNCDEIFYAGTGNLLLRDSDSITLFDVQQK RTLASVKISKVKYVIWSSDMSHVALLAKHAIMICNRKLESLCNIHENIRVKSGAWDESGV FIYTTSNHIKYALTTGDHGIIRTLDLPIYITRVKGNNVYCLDRECRPRVLTIDPTEFKFK LALINRKYDEVLHMVRNAKLVGQSIIAYLQKKGYPEVALHFVKDEKTRFGLALECGNIEI ALEAAKALDDKNCWEKLGEVALLQGNHQIVEMCYQRTKNFDKLSFLYLITGNLEKLRKMM KIAEIRKDMSGQYQNALFLGDVDERVRILKNCGQKSLAYLTAATHGINDEEESLRESFDP EKEIIPEVDPDAILLMPPPPIMPLDTNWPLLTMSKGFFEGSIANKGKGGALAADIDVDTV GGEGWGEDAELQLDEDGFVDAGENFGDEVAGKGHEEGEGWDVEEDLDLPPELDVPSGPSG SAEDGFFVPPTKGSSPAQTWCNNSQLPVDHILAGSFETAMRLLHDQVGVVNFGPFKQHFL HTFSRGRSTYQALPGLPAMYGYPQRNWKDAGLKNGLPAVGLKLNDLIQRLQLCYQFTTSG KFEEAVEKFRLILLSVPLLVVDNKQEISEAQQLITICREYIVGLSMEIDRKKLPKDTLEQ QKRICEMAAYFTHSSLQPVHMILVLRTAVNLFFKLKNFKTAATFARRLLELGPKPEVAQQ TRKILSACEKNLTDTYQLNYDMHNPFDICAASYRPIYRGKPVEKCPLSGACYSPEFKGQV CRVTTVTEIGKDVIGLRISPLQFR
Coding Sequence Details
Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None
Recommended Growth Condition:
Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:
bacterial
Marker:
spectinomycin
Bacterial Selection Condition:
100 ug/mL spectinomycin
Growth Condition:
Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments:
None
Protein Expression Results:
None
Recommended expression in:
Not Applicable
Vector Name:
pDONR223
Synonyms:
''
Description:
Gateway®, donor (entry), recombinational cloning clone, bacterial expression expression with rrnB T2 and rrnB T1 and M13 forward and attP1 and ccdB and CmR and cat_promoter-var002 and attP2 and T7 promoter and M13 reverse and SpectR and ori; spectinomycin resistance in bacterial;
Comments:
pDONR223 contains several improvements over the original Donor vector pDONR201. In pDONR223, the universal Forward (Fwd) and Reverse (Rev) sequencing primer sites flank the attP1 and attP2 recombination sites, the replication origin has been improved to increase copy number, and the selectable marker is spectinomycin resistance (instead of kanamycin resistance in pDONR201), which permits the use of either ampicillin or kanamycin resistance markers in Destination vectors.
Size (bp):
5005
Parent Vector:
None
Properties:
Gateway, bacterial expression, donor (entry), recombinational cloning
Author Name:
Invitrogen
Publications:
None
Vector Map:
Vector Sequence:
Sequence:
Vector Features:
Type
Name
Description
Start
End
CDS
ccdB
CcdB, a bacterial toxin that poisons DNA gyrase
1197
1502
CDS
SmR
confers resistance to spectinomycin and streptomycin
3471
4262
CDS
CmR
confers resistance to chloramphenicol
1844
2503
Terminator
rrnB T2
transcription terminator T2 from the E. coli rrnB gene
268
295
Terminator
rrnB T1
transcription terminator T1 from the E. coli rrnB gene
387
473
primer_bind
M13 forward
common sequencing primer, one of multiple similar variants
537
553
primer_bind
M13 reverse
common sequencing primer, one of multiple similar variants
3024
3040
promoter
cat_promoter-var002
promoter of the E. coli cat gene encoding chloramphenicol acetyltransferase
2504
2606
promoter
T7 promoter
promoter for bacteriophage T7 RNA polymerase
3001
3019
protein binding site
attP1
recombination site for the Gateway BP reaction
570
801
protein binding site
attP2
recombination site for the Gateway BP reaction
2751
2982
rep_origin
ori
high-copy-number ColE1/pMB1/pBR322/pUC origin of replication
4355
4943
