epha4-b (X. laevis ) in pDONR223 (Gateway donor/master vector)

Explanation of Terms

Gene:
Gene Symbol: 
Gene Name: 
Original Clone ID:
None
Keyword:
None
Species:
Xenopus laevis
Type:
cDNA
Vector Name:
pDONR223
Format: 
FUSION
Source:
Xenopus Gene Collection
Description:
None
Comments:
The ORFeome Collaboration, REVERSE_PRIMER: GGGGACAACTTTGTACAAGAAAGTTGGCAAAACAGGAACCATCCTGCCTT, FORWARD_PRIMER: GGGGACAACTTTGTACAAAAAAGTTGGCACCATGGCTGGGATTGTACATGG, XGC Collection: IRBH
Mutation / Discrepancy:
No / No
Publications:
None
Authors:
Center for Cancer Systems Biology
Cincinnati Children's Hospital Medical Center
MRC National Institute for Medical Research
University of Virginia School of Medicine
Aaron Zorn
David E. Hill
Michael J. Gilchrist
Todd Stukenberg
Xenopus Gene Collection
XlCD00716149
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AVAILABLE
No restriction
Special MTA: None
Insert sequence: 2958nts
Open reading frame: 1 to 2958

ATGGCTGGGATTGTACATGGCATCTTGTTTTGTGGCCTCTTTGGACTTTGTTGGGCAGTG
ACGGGATCTAGGATTTACCCAGCCAGTGAAGTTACCCTGCTGGATTCCAGGTCAGTGCAA
GGAGAGCTTGGCTGGATAGCAAGTCCTTTGGAAGGAGGGTGGGAAGAAGTGAGTATCATG
GATGAAAAGAATACACCTATACGAACTTACCAAGTCTGCAATGTCATGGAATCGAGCCAA
AACAACTGGTTACGGACTGACTGGATTCCCCGAAGTGGAGCTCAGAGAGTATATGTTGAA
ATTAAATTTACATTAAGGGACTGCAACAGCTTGCCTGGGGTAATGGGTACCTGCAAAGAG
ACCTTTAATCTGTATTATTATGAGTCCAACAATGACAAAGAGCGTTTCATTCGTGAAACC
CAGTATGTGAAAATCGATACCATTGCAGCCGATGAGAGCTTCACCCAGGTTGATATTGGG
GACCGTATTATGAAGCTGAACACTGAGGTTAGAGATGTTGGTCCACTCAGTAAGAAGGGC
TTCTACTTGGCCTTCCAGGATGTGGGAGCATGCATTGCCCTTGTGTCTGTCCGAGTGTTC
TACAAGAAGTGTCCATTGACTGTAAGAAACTTGGCACAGTTCCCTGACACCATTACAGGT
TCAGACACCTCTTCTTTGGTGGAAGTTCGAGGCTCTTGTGTAGACAACTCAGAAGAGAAA
GATGTGCCTAAGATGTACTGTGGTGCTGATGGAGAGTGGCTGGTGCCCATTGGAAACTGC
TTATGTAACGCTGGGTTTGAGGAGCACAATGGAGGATGCCAAGCCTGCAAAGTTGGATAC
TACAAAGCCCTCTCAACAGATGCAGCTTGCTCCAAGTGTCCCCCACACAGTTATGCTCTC
CGGGAAGGCTCAACCTCCTGCACCTGTGACCGTGGCTATTTTCGAGCTGACACTGACCCG
GCTTCTATGCCCTGCACTAGACCCCCATCTGCTCCTCAGAACCTAATCTCAAACGTCAAT
GAGACATCTGTGAATTTGGAGTGGAGTCCGCCACAAAATTCTGGTGGAAGGCCTGATGTC
TCCTACAATTTGGTCTGTAAAAGGTGCGGCTCTGACCTGACTCGCTGTCGGCCTTGTGGC
AGTGGAGTGCACTATAGCCCCCAGCAAAATGGCCTGAAGACTACCAAAGTGTCCATTAAT
GACCTGCAAGCCCATACTAACTACACCTTTGAGGTCTGGGCCATTAATGGTGTCTCCAAG
CAGAACCCTGAACAAGATCAGGCTGTGTCAGTTACTGTGACCACCAACCAAGCTGCTCCT
TCAACTGTAACCCAGATACAGCCAAAAGAGATCACCAGGCACAGTGTGTCTTTGACTTGG
CCAGAGCCTGAGCGAGCCAATGGGGTCATCCTGGAATACGAAGTCAAGTATTATGAGAAG
GACCAGAATGAGCGTAGTTATCGAATTGTTAAGACGGCCTCGCGAAGTGCAGACATCAAA
GGTCTCAACCCCCTTACTGGTTATGTGTTCCATGTTCGTGCTCGGACAGCTGCAGGATAT
GGGGAGTTCAGTGGACCCTTTGAGTTCACCACGAATACAGTGCCATCCCCGATGATTGGC
GAAGGAACCAGCCCCACTGTGCTTTTGGTGTCCGTGGCTGGAAGCATTGTCCTTGTGGTC
ATTCTCATTGCTGCTTTTGTCATCAGCAGAAGACGGAGTAAATACAGTAAAGCCAAGCAG
GAAGCAGACGAGGAAAAACATTTAAACCAAGGAGTAAAGACTTATGTGGACCCATTTACA
TATGAAGATCCTAACCAGGCTGTCCGCGAATTTGCAAAGGAAATCGATGCCTCTTGTATT
AAGATCGAAAAAGTAATTGGAGTAGGTGAATTTGGTGAAGTGTGCAGTGGGCGTCTGAAG
GTCCCTGGAAAGAGAGAGATTTATGTTGCAATTAAGACCCTGAAAGCTGGTTACACCGAT
AAGCAGAGGAGAGACTTTCTCAGTGAGGCCAGCATCATGGGTCAGTTTGACCATCCTAAC
ATCATCCACCTTGAAGGTGTTGTCACCAAATGCAAGCCAGTGATGATAATCACCGAGTAC
ATGGAGAACGGATCCTTGGATGCATTCCTTCGGAAGAATGATGGTCGGTTTACAGTAATA
CAGCTTGTGGGAATGCTGCGTGGCATTGGGTCTGGAATGAAATACCTTTCGGACATGAGT
TATGTGCACCGGGACCTAGCTGCTCGTAATATCCTTGTGAACAGCAACCTAGTCTGTAAG
GTCTCTGACTTTGGCATGTCTCGGGTGCTGGAAGATGACCCTGAAGCAGCCTACACAACC
AGGGGTGGCAAGATCCCAATCCGATGGACTGCACCAGAAGCTATTGCATACAGGAAATTC
ACTTCTGCTAGTGATGTCTGGAGCTATGGCATTGTCATGTGGGAAGTCATGTCTTATGGA
GAGAGGCCTTACTGGGATATGTCCAATCAAGATGTAATTAAAGCAATCGAGGAAGGATAC
AGGCTGCCCCCGCCTATGGACTGCCCTATTGCACTGCATCAGCTCATGTTGGACTGCTGG
CAGAAGGATCGTAGTGACCGACCAAAATTTGGGCAGATAGTCAGCATGCTGGATAAACTC
ATCAGGAACCCAAACAGTTTGAAAAGGACTGGGCTGGAGAATTCAAGCAGAACAAATACG
GCTCTGCTGGACCCCAGTTCGCCTGAATGGTCCCAGGTGGCTTCTGTGCTTGATTGGCTG
CAGGCCATCAAAATGGAAAGATACAAAGATAACTTCACAGCAGCCGGATACACCAGCTTG
GAAGCAGTCGTGCATGTGAATCAAGATGACTTGACCAGGATTGGCATTAGTTCCCCTTCA
CACCAGAACAAGATCCTGAGCAGCGTCCAAGGAATGAGGACCCAGTTGCAGCAAATGCAA
GGCAGGATGGTTCCTGTTTGA

Protein sequence predicted based on nucleotide sequence

MAGIVHGILFCGLFGLCWAVTGSRIYPASEVTLLDSRSVQGELGWIASPLEGGWEEVSIM
DEKNTPIRTYQVCNVMESSQNNWLRTDWIPRSGAQRVYVEIKFTLRDCNSLPGVMGTCKE
TFNLYYYESNNDKERFIRETQYVKIDTIAADESFTQVDIGDRIMKLNTEVRDVGPLSKKG
FYLAFQDVGACIALVSVRVFYKKCPLTVRNLAQFPDTITGSDTSSLVEVRGSCVDNSEEK
DVPKMYCGADGEWLVPIGNCLCNAGFEEHNGGCQACKVGYYKALSTDAACSKCPPHSYAL
REGSTSCTCDRGYFRADTDPASMPCTRPPSAPQNLISNVNETSVNLEWSPPQNSGGRPDV
SYNLVCKRCGSDLTRCRPCGSGVHYSPQQNGLKTTKVSINDLQAHTNYTFEVWAINGVSK
QNPEQDQAVSVTVTTNQAAPSTVTQIQPKEITRHSVSLTWPEPERANGVILEYEVKYYEK
DQNERSYRIVKTASRSADIKGLNPLTGYVFHVRARTAAGYGEFSGPFEFTTNTVPSPMIG
EGTSPTVLLVSVAGSIVLVVILIAAFVISRRRSKYSKAKQEADEEKHLNQGVKTYVDPFT
YEDPNQAVREFAKEIDASCIKIEKVIGVGEFGEVCSGRLKVPGKREIYVAIKTLKAGYTD
KQRRDFLSEASIMGQFDHPNIIHLEGVVTKCKPVMIITEYMENGSLDAFLRKNDGRFTVI
QLVGMLRGIGSGMKYLSDMSYVHRDLAARNILVNSNLVCKVSDFGMSRVLEDDPEAAYTT
RGGKIPIRWTAPEAIAYRKFTSASDVWSYGIVMWEVMSYGERPYWDMSNQDVIKAIEEGY
RLPPPMDCPIALHQLMLDCWQKDRSDRPKFGQIVSMLDKLIRNPNSLKRTGLENSSRTNT
ALLDPSSPEWSQVASVLDWLQAIKMERYKDNFTAAGYTSLEAVVHVNQDDLTRIGISSPS
HQNKILSSVQGMRTQLQQMQGRMVPV

Coding Sequence Details

Insert Sequence Verified?:
Y
Verification Method:
End read sequencing
Mutations:
None
Discrepancy:
None
5' Linker Sequence:
None
3' Linker Sequence:
None

Recommended Growth Condition:

Distributed in bacterial strain:
DH5-alpha T1 phage resistant
Antibiotic Selection:
Host Type:  bacterial    Marker:  spectinomycin
Bacterial Selection Condition: 100 ug/mL spectinomycin
Growth Condition: Growth at 37 degrees C in LB supplemented with 100 ug/mL spectinomycin.
Comments: None
Protein Expression Results:
None
Recommended expression in:
Not Applicable